Results 51 to 60 of about 1,751,397 (315)

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Methods and Factors Influencing In Vitro Propagation Efficiency of Ornamental Tuberose (Polianthes Species): A Systematic Review of Recent Developments and Future Prospects

Horticulturae, 2022
In vitro propagation greatly boosts the propagation rate and allows for the development of disease-free plants. In the near future, new in vitro propagation methods could make it easier to produce plants true to type on a wide scale and to use genetic ...
Mukesh Kumar, Ujjwal Sirohi, Sunil Malik, Satendra Kumar, Gaurav Kumar Ahirwar, Veena Chaudhary, Manoj Kumar Yadav, Jitender Singh, Arvind Kumar, Virendra Pal, Satya Prakash   +10 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Production and characterization of extracellular lipase from Calvatia gigantea

, 1992
A number of factors affecting production of extracellular lipase by the edible fungus Calvatia gigantea were investigated. Consecutive optimization of carbon and nitrogen sources, initial pH of culture medium and growth temperature resulted in an ...
Christakopoulos, P, Kekos, Dimitris,, Christakopoulos, Paul, Macris, Basil J.,, Macris, BJ, Tzia, Constantina,, Tzia, C, Kekos, D   +7 more
core   +1 more source

Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats

مجله دانشگاه علوم پزشکی بیرجند, 2022
Background and Aims: Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases.
Farzaneh Jasbinia, Majid Hassanpourezatti   +1 more
doaj  

Cultivation of Cells in a Physiological Plasmax Medium Increases Mitochondrial Respiratory Capacity and Reduces Replication Levels of RNA Viruses

Antioxidants, 2021
Changes in metabolic pathways are often associated with the development of various pathologies including cancer, inflammatory diseases, obesity and metabolic syndrome.
Michail V. Golikov, Inna L. Karpenko, Anastasiya V. Lipatova, Olga N. Ivanova, Irina T. Fedyakina, Viktor F. Larichev, Natalia F. Zakirova, Olga G. Leonova, Vladimir I. Popenko, Birke Bartosch, Sergey N. Kochetkov, Olga A. Smirnova, Alexander V. Ivanov   +12 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

In Vitro Regeneration Protocol for Securidaca longepedunculata Fresen., a Threatened Medicinal Plant within the Region of Lubumbashi (Democratic Republic of the Congo)

Conservation, 2023
Securidaca longepedunculata Fresen. is an overexploited forest species in the Lubumbashi region (south-eastern DR Congo), as its roots are highly valued in traditional medicine.
Magnifique Chuimika Mulumbati, Mario Godoy Jara, Louis Baboy Longanza, Jan Bogaert, Stefaan Werbrouck, Yannick Useni Sikuzani, Michel Mazinga Kwey   +6 more
doaj   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]

, 2011
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
M. O. Riehle, J.R. Higginson (7130108), Higginson, J.R., Donoghue, P.S., T. Sun, Nikolaj Gadegaard (1292763), J. R. Higginson, N. Gadegaard, Riehle, M.O., Mathis O. Riehle (1292769), Barnett, S.C., Sun, T., Tao Sun (1255119), Peter S. Donoghue (7128581), S. C. Barnett, Gadegaard, N., P. S. Donoghue, Susan C. Barnett (7128590)   +17 more
core   +1 more source