Results 51 to 60 of about 149 (149)
Molecular Oncology, EarlyView.TRIM24 and TRIM28 are androgen receptor (AR) coregulators which exhibit increased expression with cancer progression. Both TRIM24 and TRIM28 combine to influence the response of castrate‐resistant prostate cancer (CRPC) cells to AR inhibitors by mediating AR signalling, regulation of MYC and upregulating VEGF to promote angiogenesis. Castrate‐resistant
Damien A. Leach +8 more
wiley +1 more source
Understanding and measuring mechanical signals in the tumor stroma
FEBS Open Bio, EarlyView.This review discusses cancer‐associated fibroblast subtypes and their functions, particularly in relation to extracellular matrix production, as well as the development of 3D models to study tumor stroma mechanics in vitro. Several quantitative techniques to measure tissue mechanical properties are also described, to emphasize the diagnostic and ...
Fàtima de la Jara Ortiz +3 more
wiley +1 more source
Mapping Hsp104 interactions using cross‐linking mass spectrometry
FEBS Open Bio, EarlyView.This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.By using an electrical pulse stimulation (EPS)‐based in vitro exercise model and chemical activation/inhibition of mechanosensitive Piezo1 channels, we explored changes in protein synthetic response in cultured C2C12 myotubes. Our data showed that application of Piezo1 activator (Yoda1) during EPS significantly enhances the rate of protein synthesis in
Natalia A. Vilchinskaya +3 more
wiley +1 more source
Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria
FEBS Open Bio, EarlyView.The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley +1 more source
FEBS Open Bio, EarlyView.The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik +3 more
wiley +1 more source