Results 41 to 50 of about 2,309,175 (337)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Neuroelectronic interfacing with cultured multielectrode arrays toward a cultured probe [PDF]

, 2001
Efficient and selective electrical stimulation and recording of neural activity in peripheral, spinal, or central pathways requires multielectrode arrays at micrometer scale.
Buitenweg, Jan, Heida, Ciska, Lakke, Egbert, Marani, Enrico, Mouveroux, Jean-Marie, Ruardij, Teun, Rutten, Wim   +6 more
core   +3 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Superior survival of ex vivo cultured human reticulocytes following transfusion into mice

Haematologica, 2017
The generation of cultured red blood cells from stem cell sources may fill an unmet clinical need for transfusion-dependent patients, particularly in countries that lack a sufficient and safe blood supply.
Sabine Kupzig, Stephen F. Parsons, Elinor Curnow, David J. Anstee, Allison Blair   +4 more
doaj   +1 more source

Novel culture media enhances mononuclear cells from patients with chronic limb-threatening ischemia to increase vasculogenesis and anti-inflammatory effect

Stem Cell Research & Therapy, 2021
Background Quality and Quantity culture media (QQ culture media) was reported to enhance vasculogenesis and angiogenesis function of mononuclear cells (MNCs) from healthy volunteers.
Nuttapol Chruewkamlow, Kanin Pruekprasert, Phakawan Phutthakunphithak, Onchira Acharayothin, Tossapol Prapassaro, Kiattisak Hongku, Suteekhanit Hahtapornsawan, Nattawut Puangpunngam, Khamin Chinsakchai, Chumpol Wongwanit, Chanean Ruangsetakit, Nuttawut Sermsathanasawadi   +11 more
doaj   +1 more source

Mode of cell death of co-cultured dental mesenchymal stem cells following exposure to endodontic irrigating solutions

Dentistry 3000, 2022
Aims:  This in vitro study aimed to determine the mode of cell death of co-cultured  dental mesenchymal stem cells following exposure to different irrigating solutions.
Eshagh Ali Saberi, Narges Farhad Mollashahi, Mahboobe Ahmadi, Arezoo Pirhaji   +3 more
doaj   +1 more source

Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells [PDF]

, 2019
The gingival epithelium is a physical and immunological barrier to the microbiota of the oral cavity, which interact through soluble mediators with the immune cells that patrol the tissue at the gingival epithelium.
Bradshaw, David, Brown, Jason L., Butcher, John, Culshaw, Shauna, Delaney, Chris, Johnston, William, Khan, Shaz, Rajendran, Ranjith, Ramage, Gordon   +8 more
core   +4 more sources

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source