Results 41 to 50 of about 1,450,054 (334)

CARS and SHG microscopy to follow the collagen production in living human corneal fibroblasts and mesenchymal stem cells in fibrin gel 3D cultures [PDF]

, 2011
Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with second harmonic generation (SHG) technique in order to follow the early stage of stem cell differentiation within a 3D scaffold. CARS microscopy can detect lipid membranes and droplet compartments in living cells and SHG microscopy enables a strong imaging contrast for molecules ...
arxiv   +1 more source

Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase

FEBS Letters, EarlyView.
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri, Haruka Tachibana, Daisuke Kawasaki, Taichi Chisuga, Toma Kashima, Shinya Fushinobu, Fumitaka Kudo, Tadashi Eguchi, Akimasa Miyanaga   +8 more
wiley   +1 more source

Homogenization of Mammalian Cultured Cells

The Scientific World Journal, 2002
Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any ...
John Graham
doaj   +1 more source

Ablation of LRP6 in alpha‐smooth muscle actin‐expressing cells abrogates lung inflammation and fibrosis upon bleomycin‐induced lung injury

FEBS Letters, EarlyView.
Low‐density lipoprotein receptor‐related protein 6 (LRP6) is a key receptor for the Wnt antagonist Dickkopf1 (DKK1). DKK1 protein expression is induced in a bleomycin (BLM)‐induced lung injury model. We show that DKK1 induces proinflammatory and profibrotic genes in lung fibroblasts.
Eun‐Ah Sung, Mikhail G. Dozmorov, SuJeong Song, Theingi Aung, Min Hee Park, Patricia J. Sime, Wook‐Jin Chae   +6 more
wiley   +1 more source

HMG-CoA reductase regulation: use of structurally diverse first half-reaction squalene synthetase inhibitors to characterize the site of mevalonate-derived nonsterol regulator production in cultured IM-9 cells

Journal of Lipid Research, 1999
The activity of HMG-CoA reductase (HMGR) is tightly regulated, in part through post-transcriptional mechanisms that are mediated by nonsterol products of mevalonate metabolism.
Stephen F. Petras, Saralyn Lindsey, H. James Harwood, Jr.   +2 more
doaj  

Distinct cell stress responses induced by ATP restriction in quiescent human fibroblasts

Frontiers in Genetics, 2016
Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly little is known about how quiescent cells respond to energetic and metabolic challenges.
Nirupama Yalamanchili, Andres Kriete, David J. Alfego, Kelli Mayo Danowski, Ulrich Rodeck, Csaba Kari   +5 more
doaj   +1 more source

Novel culture media enhances mononuclear cells from patients with chronic limb-threatening ischemia to increase vasculogenesis and anti-inflammatory effect

Stem Cell Research & Therapy, 2021
Background Quality and Quantity culture media (QQ culture media) was reported to enhance vasculogenesis and angiogenesis function of mononuclear cells (MNCs) from healthy volunteers.
Nuttapol Chruewkamlow, Kanin Pruekprasert, Phakawan Phutthakunphithak, Onchira Acharayothin, Tossapol Prapassaro, Kiattisak Hongku, Suteekhanit Hahtapornsawan, Nattawut Puangpunngam, Khamin Chinsakchai, Chumpol Wongwanit, Chanean Ruangsetakit, Nuttawut Sermsathanasawadi   +11 more
doaj   +1 more source

Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells

FEBS Letters, EarlyView.
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga, Kazuki Harada, Takuya Yamauchi, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Takashi Tsuboi   +6 more
wiley   +1 more source

Cells of Matter—In Vitro Models for Myotonic Dystrophy

Frontiers in Neurology, 2018
Myotonic dystrophy type 1 (DM1 also known as Steinert disease) is a multisystemic disorder mainly characterized by myotonia, progressive muscle weakness and wasting, cognitive impairments, and cardiac defects. This autosomal dominant disease is caused by
Magdalena Matloka, Arnaud F. Klein, Frédérique Rau, Denis Furling   +3 more
doaj   +1 more source

Distinguishing cancerous from non-cancerous cells through analysis of electrical noise [PDF]

, 2007
Since 1984, electric cell-substrate impedance sensing (ECIS) has been used to monitor cell behavior in tissue culture and has proven sensitive to cell morphological changes and cell motility. We have taken ECIS measurements on several cultures of non-cancerous (HOSE) and cancerous (SKOV) human ovarian surface epithelial cells. By analyzing the noise in
arxiv   +1 more source