Results 61 to 70 of about 6,073,818 (310)

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Homogenization of Mammalian Cultured Cells

The Scientific World Journal, 2002
Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any ...
John Graham
doaj   +1 more source

ImmunoFISH for Adherent Cultured Mammalian Cells

Bio-Protocol, 2013
This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.
Francesca Rossiello, Marzia Fumagalli, Fabrizio di Fagagna   +2 more
doaj   +1 more source

Slow Turning Lateral Vessel Bioreactor Improves Embryoid Body Formation and Cardiogenic Differentiation of Mouse Embryonic Stem Cells. [PDF]

, 2013
Embryonic stem cells (ESCs) have the ability to form aggregates, which are called embryoid bodies (EBs). EBs mimic early embryonic development and are commonly produced for cardiomyogenesis.
Tharasanit, T., Biology of Reproductive Cells, Pirity, M., Rungarunlert, Sasitorn, Klincumhom, Nuttha, Rungarunlert, S., Dinnyés, András, Tharasanit, Theerawat, Dep Gezondheidszorg Landbouwhuisdieren, Klincumhom, N., Techakumphu, M., Pirity, Melinda, Dinnyes, A.   +12 more
core   +2 more sources

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Microgravity-induced apoptosis in cultured glial cells

European Journal of Histochemistry, 2010
Apoptosis is a form of naturally occurring cell death that plays fundamental roles during embryonic developement. In adults, it neatly disposes of cells damaged by injuries provoked by external causes such as UV radiation, ionisation and heat shock ...
BM Uva, MA Masini, M Sturla, F Bruzzone, M Giuliani, G Tagliafierro, F Strollo   +6 more
doaj   +1 more source

Correlation of memory T cell responses against TRAP with protection from clinical malaria, and CD4+ CD25high T cells with susceptibility in Kenyans [PDF]

, 2008
Background - Immunity to malaria develops naturally in endemic regions, but the protective immune mechanisms are poorly understood. Many vaccination strategies aim to induce T cells against diverse pre-erythrocytic antigens, but correlates of protection ...
Magdalena Plebanski, Stephen M. Todryk, Todryk, Stephen M., Plebanski Magdalena, Bejon, P, Hill, Adrian, Tabitha Mwangi, Kevin Marsh, Urban Britta, Flanagan Katie L., Mwangi Tabitha, Plebanski, Magdalena, Urban, B, Britta Urban, Todryk, Stephen, Hill Adrian V. S., Urban, Britta, Katie L Flanagan, Flanagan, KL, Hill, AV, Philip Bejon, Bejon, Philip, Marsh Kevin, Marsh, K, Mwangi, T, Mwangi, Tabitha, Flanagan, Katie, Hill, Adrian V. S., Bejon Philip, Plebanski, M, Katie L. Flanagan, Adrian V S Hill, Todryk Stephen M., Adrian V. S. Hill, Stephen M Todryk, Marsh, Kevin, Todryk, SM, Flanagan, Katie L.   +37 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

High conductance anion channel in Schwann cell vesicles from rat spinal roots [PDF]

, 1992
Potassium uptake, possibly together with chloride, is one of the presumed functions of Schwann cells in the peripheral nervous system. However, the presence of chloride channels has not been demonstrated in adult Schwann cells.
Quasthoff, Stefan, Stefan Quasthoff, Peter Grafe, Strupp, Michael, Grafe, Peter, Michael Strupp   +5 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source