Results 81 to 90 of about 2,231,845 (305)

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Impaired bone marrow homing of cytokine-activated CD34⁺ cells in the NOD/SCID model [PDF]

, 2004
The reduced engraftment potential of hematopoietic stem/progenitor cells (HSPCs) after exposure to cytokines may be related to the impaired homing ability of actively cycling cells. We tested this hypothesis by quantifying the short-term horning of human
Ahmed, F, Blundell, MP, Fahey, A, Ings, SJ, Linch, DC, Pizzey, AR, Thrasher, AJ, Ye, HT, Yong, KL   +8 more
core  

Comparative Study of Xenobiotic-Free Media for the Cultivation of Human Limbal Epithelial Stem/Progenitor Cells. [PDF]

, 2017
The culture of human limbal epithelial stem/progenitor cells (LSCs) in the presence of animal components poses the risk of cross-species contamination in clinical applications.
Chen, Luxia, Deng, Sophie X, González, Sheyla   +2 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Differential Response to Cisplatin between Co-cultured Cells and Pure Cultured Cells Based on Single-cell RNA Sequencing of Three-dimensional-cultured Breast Cancer Cells

Frontiers in Bioscience-Landmark
Objective: The current study aimed to develop an experimental approach for the direct co-culture of three-dimensional breast cancer cells using single-cell RNA sequencing (scRNA-seq ...
Shuqing Yang, Peixian Chen, Xiaofan Mao, KaiRong Lin, Wei Li, Tiancheng He, Huiqi Huang, AiGuo Wu, Wei Luo, Guolin Ye, Guangyu Yao, Dan Zhou   +11 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Increased hepatic VLDL secretion, lipogenesis, and SREBP-1 expression in the corpulent JCR:LA-cp rat

Journal of Lipid Research, 2001
The corpulent JCR:LA-cp rat (cp/cp) is a useful model for study of the metabolic consequences of obesity and hyperinsulinemia. To assess the effect of hyperinsulinemia on VLDL secretion in this model, we measured rates of secretion of VLDL in perfused ...
Marshall B. Elam, Henry G. Wilcox, Lauren M. Cagen, Xiong Deng, Rajendra Raghow, Poonam Kumar, Murray Heimberg, James C. Russell   +7 more
doaj   +1 more source

Introduction of Macromolecules into Bovine Adrenal Medullary Chromaffin Cells and Rat Pheochromocytoma Cells (PC12) by Permeabilization with Streptolysin O: Inhibitory Effect of Tetanus Toxin on Catecholamine Secretion [PDF]

, 1989
Conditions are described for controlled plasma membrane permeabilization of rat pheochromocytoma cells (PC12) and cultured bovine adrenal chromaffin cells by Streptolysin O (SLO).
Ahnert-Hilger G., Ahnert-Hilger G., Ahnert-Hilger G., Bader M. F., Bhakdi S., Bhakdi S., Bhakdi S., Bittner M. A., Brooks J. C., Cazalis M., Duncan J. L., Flodgaard H., Gudrun Ahnert-Hilger, Habermann E., Hugo F., Jahn R., Knight D. E, Manfred Gratzl, Marie-France Bader, Peppers S. C., Rehm H., Sarafian T., Sillen L. G., Simon W., Smyth C. I., Sontag J. M., Sternberger A., Sucharit Bhakdi, Walton K. M., Wilson S. P.   +29 more
core   +1 more source

Microvascular Endothelial Cell Culture.

Journal of Investigative Dermatology, 1989
Microvascular endothelial cells play a central role in inflammation, tumor metastasis, and wound healing. Methods to study these processes in vitro using cells isolated from adult skin, from the inner and the outer segments of the neontal foreskin, and from experimental animals are reviewed.
openaire   +2 more sources

Cell culture

, 2023
Published by MDPI ...
Weiskirchen, Sabine, Weiskirchen, Ralf, Schröder, Sarah K., Buhl, Eva Miriam   +3 more
openaire   +2 more sources