Results 61 to 70 of about 80,443 (263)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Adsorption and anti-corrosion properties od cuttingfluids for shear blades of glass-forming machines

Zaštita Materijala
The article presents the studies on the adsorption of water-miscible oil-containing cutting fluids on the R6M5 high-speed steel surface. The fluids can be used for the shear blades of drop feeders in glass-forming machines.
Ponomarev Daniil Andreevich, Marina Pavlovna Krasnovskikh, Solovyova Anastasia Dmitrievna, Shcherban Marina Grigorievna   +3 more
doaj   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Numerical modeling and simulation of drilling cutting transport in horizontal wells

Journal of Petroleum Exploration and Production Technology, 2018
Cutting transport is an important goal in drilling operation especially in horizontal and deviated wells since it can cause problems such as stuck pipe, circulation loss and high torque and drag.
Ali Zakerian, Siyamak Sarafraz, Amir Tabzar, Nassim Hemmati, Seyed Reza Shadizadeh   +4 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Hydrated Seal Lip for Live Center in Machine Tools

Frontiers in Mechanical Engineering, 2018
When performing a cutting operation, a water-soluble cutting fluid may be used for cooling. Labyrinth and oil seals are commonly used in the rotating centers of machine tools to prevent the cutting fluid and dust from entering the inner parts.
Tetsuya Akiyama, Yuki Yoshioka, Takuro Honda, Yuta Nakashima, Yoshitaka Nakanishi   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Enhancing high-speed cutting performance of Inconel 718 superalloy via heat inhibiting with a bio-textured PcBN tool

Journal of Materials Research and Technology
Ni-based superalloy exhibits poor machinability due to low thermal conductivity and work hardening, leading to high cutting temperatures that accelerate tool wear and degrade surface quality.
Shilong Chen, Dehui Liu, Xirui Liu, Zihao Lin, Tianye Jin, Junyun Chen, Chuanzhen Huang   +6 more
doaj   +1 more source

Impact of nanoparticles on vegetable oil as a cutting fluid with fractional ramped analysis. [PDF]

Sci Rep, 2023
Hasin F, Ahmad Z, Ali F, Khan N, Khan I, Eldin SM.   +5 more
europepmc   +1 more source