Results 121 to 130 of about 718,654 (353)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Investigation of the effects of machining parameters on cutting conditions during orthogonal turning of austenite stainless steel

open access: yesProduction Engineering Archives
The 1.4306 austenite stainless steel has been prominently utilized as a material in the automotive and aerospace industry. Considerable interest has been garnered in the machinability of stainless steel owing to its high strength and poor thermal ...
Kónya Gábor   +3 more
doaj   +2 more sources

Analysis of Thermal Aspect in Hard Turning of AISI 52100 Alloy Steel Under Minimal Cutting Fluid Environment Using FEM

open access: yesApplied Mechanics
This paper describes a simulation study on the hard turning of AISI 52100 alloy steel with coated carbide tools under minimal cutting fluid conditions using the commercial software AdvantEdge.
Sandip Mane   +5 more
doaj   +1 more source

A study on cryogenic supercritical carbon dioxide coolant delivery technique when machining of AISI 1045 steel [PDF]

open access: yes, 2018
The machining operation involves a material removal process and develop high temperature due to the friction force. The heat generated at the region of cutting edge has critical influences on machining process.
Abd Rahim, Erween   +4 more
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Study on milling of super elasto-plastic β type titanium alloy (Verification of processing characteristics and selection of optimum cutting conditions)

open access: yesNihon Kikai Gakkai ronbunshu, 2017
Recently, a titanium alloy has been as biomaterials that have stable mechanical property and superior biocompatibility. But the titanium alloy generally has higher young’s modulus in comparison with cortical bone, then bone resorption was occurred by ...
Hideharu KATO   +4 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

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