Coupling antibody to cyanogen bromide-activated sepharose. [PDF]
Methods Enzymol, 2014 Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification.
Kavran JM, Leahy DJ.
europepmc +6 more sources
Daily bursts of biogenic cyanogen bromide (BrCN) control biofilm formation around a marine benthic diatom. [PDF]
Proc Natl Acad Sci U S A, 2012 Vanelslander B +7 more
europepmc +4 more sources
Nickel-Catalyzed Reductive Cyanation of Aryl Halides and Epoxides with Cyanogen Bromide [PDF]
MoleculesNitriles are valuable compounds because they have widespread applications in organic chemistry. This report details the nickel-catalyzed reductive cyanation of aryl halides and epoxides with cyanogen bromide for the synthesis of nitriles.
Yu-Juan Wu +3 more
doaj +2 more sources
Are Methionine Sulfoxide-Containing Proteins Related to Seed Longevity? A Case Study of Arabidopsis thaliana Dry Mature Seeds Using Cyanogen Bromide Attack and Two-Dimensional-Diagonal Electrophoresis [PDF]
Plants, 2022 In recent years, several reports pointed out the role of protein oxidation in seed longevity, notably regarding the oxidation of methionine (Met) residues to methionine sulfoxide (MetO) in proteins.
Ewa Marzena Kalemba +4 more
doaj +2 more sources
Thioredoxin 2: cleavage with cyanogen bromide. [PDF]
European Journal of Biochemistry, 1967 A simple and highly reproducible method for the preparation of thioredoxin from Escherichia coli B was worked out. On treatment with cyanogen bromide thioredoxin was cleaved into two peptides which were separated by chromatography on Sephadex G-50.
A. Holmgren, P. Reichard
semanticscholar +4 more sources
Enfuvirtide biosynthesis in thermostable chaperone-based fusion [PDF]
Biotechnology Reports, 2022 Synthetic peptides are in high demand as biologically active substances. Solid phase synthesis is the primary method of peptide production. However, it has drawbacks: large amount of chemical waste and rapid increase in price with peptide length ...
Vladimir Zenin +3 more
doaj +2 more sources
Conformational Properties of Cyanogen Bromide-cleaved Glucagon
Journal of Biological Chemistry, 1972 Abstract The circular dichroism of the cyanogen bromide peptide of glucagon, measured in dilute aqueous solution, in chloroethanol, and in urea is similar to that of glucagon. However, in more concentrated aqueous solutions the cyanogen bromide peptide does not rapidly aggregate to structures of higher helical content as does glucagon.
Richard M. Epand
openaire +4 more sources
C-terminal protein characterization by mass spectrometry: isolation of C-terminal fragments from cyanogen bromide-cleaved protein. [PDF]
J Biomol Tech, 2014 Nika H, Hawke DH, Angeletti RH.
europepmc +3 more sources
A study of the mechanism of cyanogen bromide activation of cellulose [PDF]
Biotechnology and Bioengineering, 1972 R. O. Stasiw +5 more
openaire +4 more sources
Introducing a New Method for Purification of Human IL-4 by Substitution of a Single Amino Acid in IL-4 Protein Sequence [PDF]
Iranian Journal of Immunology, 2022 Background: It is advantageous to develop an effective purification procedure to produce recombinant protein drugs (rPDs) without any tags. To remove N- or C-terminus tags from the rPDs, several cleavage site-based endopeptidases were used.
Mohsen Mazloomrezaei +6 more
doaj +1 more source