Coupling Antibody to Cyanogen Bromide-Activated Sepharose [PDF]
Methods Enzymol, 2014 Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification.
Jennifer M, Kavran, Daniel J, Leahy
semanticscholar +4 more sources
Nickel-Catalyzed Reductive Cyanation of Aryl Halides and Epoxides with Cyanogen Bromide [PDF]
MoleculesNitriles are valuable compounds because they have widespread applications in organic chemistry. This report details the nickel-catalyzed reductive cyanation of aryl halides and epoxides with cyanogen bromide for the synthesis of nitriles.
Yu-Juan Wu +3 more
doaj +2 more sources
Are Methionine Sulfoxide-Containing Proteins Related to Seed Longevity? A Case Study of Arabidopsis thaliana Dry Mature Seeds Using Cyanogen Bromide Attack and Two-Dimensional-Diagonal Electrophoresis [PDF]
Plants, 2022 In recent years, several reports pointed out the role of protein oxidation in seed longevity, notably regarding the oxidation of methionine (Met) residues to methionine sulfoxide (MetO) in proteins.
Ewa Marzena Kalemba +4 more
doaj +2 more sources
Thioredoxin 2: Cleavage with Cyanogen Bromide [PDF]
European Journal of Biochemistry, 1967 A simple and highly reproducible method for the preparation of thioredoxin from Escherichia coli B was worked out.On treatment with cyanogen bromide thioredoxin was cleaved into two peptides which were separated by chromatography on Sephadex G‐50.
A, Holmgren, P, Reichard
openaire +3 more sources
Enfuvirtide biosynthesis in thermostable chaperone-based fusion [PDF]
Biotechnology Reports, 2022 Synthetic peptides are in high demand as biologically active substances. Solid phase synthesis is the primary method of peptide production. However, it has drawbacks: large amount of chemical waste and rapid increase in price with peptide length ...
Vladimir Zenin +3 more
doaj +2 more sources
Quality Control and Regulatory Scientific Research on Collagen‐Based Medical Devices [PDF]
Cell ProlifCell Proliferation, Volume 59, Issue 1, January 2026.
Rui Wang +4 more
wiley +2 more sources
Inorganic materials as supports for covalent enzyme immobilization: Methods and mechanisms [PDF]
Molecules, 2014 Several inorganic materials are potentially suitable for enzymatic covalent immobilization, by means of several different techniques. Such materials must meet stringent criteria to be suitable as solid matrices: complete insolubility in water, reasonable
SANJUST, ENRICO, ZUCCA, PAOLO
core +4 more sources
Common Food Additives Inhibit Carbonic Anhydrase Activity [PDF]
Journal of Biochemical and Molecular Toxicology, Volume 39, Issue 9, September 2025.Food additives inhibit carbonic anhydrases ABSTRACT Few studies about the effects of food additives on human enzymes exist. The effects of 20 food additives (12 colorants, 2 antioxidants, 3 sweeteners, 2 preservatives, and 1 acidity regulator) on bovine carbonic anhydrase (bCA) and especially human isoenzymes hCAI and hCAII isoenzyme, a highly vital ...
Ayça Aktaş Karaçelik +3 more
wiley +2 more sources
Daily bursts of biogenic cyanogen bromide (BrCN) control biofilm formation around a marine benthic diatom [PDF]
Proc Natl Acad Sci U S A, 2012 B. Vanelslander +7 more
semanticscholar +2 more sources