Results 51 to 60 of about 152,744 (302)

Effects of Phosphorylation of Threonine 160 on Cyclin-dependent Kinase 2 Structure and Activity [PDF]

Journal of Biological Chemistry, 1999
We have prepared phosphorylated cyclin-dependent protein kinase 2 (CDK2) for crystallization using the CDK-activating kinase 1 (CAK1) from Saccharomyces cerevisiae and have grown crystals using microseeding techniques. Phosphorylation of monomeric human CDK2 by CAK1 is more efficient than phosphorylation of the binary CDK2-cyclin A complex ...
Brown, Nicholas, Noble, Martin E.M., Lawrie, Alison, Morris, May, Tunnah, Paul, Divita, Gilles, Johnson, Louise, Endicott, Jane   +7 more
openaire   +5 more sources

Ubiquitination of transcription factors in cancer: unveiling therapeutic potential

Molecular Oncology, EarlyView.
In cancer, dysregulated ubiquitination of transcription factors contributes to the uncontrolled growth and survival characteristics of tumors. Tumor suppressors are degraded by aberrant ubiquitination, or oncogenic transcription factors gain stability through ubiquitination, thereby promoting tumorigenesis.
Dongha Kim, Hye Jin Nam, Sung Hee Baek
wiley   +1 more source

Anticancer activity of Elsholtzia winitiana var. dongvanensis Phuong on gastric cancer cells: phytochemical profiling, cell proliferation inhibition, senescence induction, and cell cycle arrest

Beni-Suef University Journal of Basic and Applied Sciences
Background Elsholtzia, a genus within the Lamiaceae family, comprises 42 species predominantly distributed across East and Southeast Asia. Elsholtzia winitiana var. dongvanensis Phuong (EWD) is a subspecies of Elsholtzia winitiana endemic to the Dong Van–
Van Hung Hoang, Thi Thanh Huong Le, Phu Hung Nguyen, Duy Hung Nguyen, Quang Tan Luc, Thi Kieu Oanh Nguyen, Khac Hung Do   +6 more
doaj   +1 more source

A cyclin-dependent kinase that promotes cytokinesis through modulating phosphorylation of the carboxy terminal domain of the RNA Pol II Rpb1p sub-unit. [PDF]

PLoS ONE, 2007
In Schizosaccharomyces pombe, the nuclear-localized kinase, Lsk1p, promotes cytokinesis by positively regulating the Septation Initiation Network (SIN).
Jim Karagiannis, Mohan K Balasubramanian
doaj   +1 more source

Cyclin-dependent kinase 2 (Cdk2) is required for centrosome duplication in mammalian cells [PDF]

Current Biology, 1999
Centrosome duplication is indispensable for the formation of the bipolar mitotic spindle. Surprisingly, even if DNA replication or mitosis is inhibited, centrosome duplication can still occur [1] [2] [3] [4] [5]. Thus, it remains unknown how centrosome duplication is coordinated with the cell cycle.
Ken Hayashi, Eisuke Nishida, Yutaka Matsumoto   +2 more
openaire   +3 more sources

Targeted protein degradation in oncology: novel therapeutic opportunity for solid tumours?

Molecular Oncology, EarlyView.
Current anticancer therapies are limited by the occurrence of resistance and undruggability of most proteins. Targeted protein degraders are novel, promising agents that trigger the selective degradation of previously undruggable proteins through the recruitment of the ubiquitin–proteasome machinery. Their mechanism of action raises exciting challenges,
Noé Herbel, Sophie Postel‐Vinay
wiley   +1 more source

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

Genomics‐led approach to drug testing in models of undifferentiated pleomorphic sarcoma

Molecular Oncology, EarlyView.
GA text Genomic data from undifferentiated pleomorphic sarcoma patients and preclinical models were used to inform a targeted drug screen. Selected compounds were tested in 2D and 3D cultures of UPS cell lines. A combination of trametinib and infigratinib was synergistic in the majority of UPS cell lines tested, which was further confirmed in an ex ...
Piotr J. Manasterski, Molly R. Danks, John P. Thomson, Morwenna Muir, Martin Lee, John C. Dawson, Ana T. Amaral, Juan Diaz‐Martin, David S. Moura, Javier Martin‐Broto, Ali Alsaadi, Donald M. Salter, Ailsa J. Oswald, Graeme Grimes, Larry Hayward, Ted R. Hupp, Karen Sisley, Paul H. Huang, Neil O. Carragher, Valerie G. Brunton   +19 more
wiley   +1 more source

Gauchos and ochos: a Wee1-Cdk tango regulating mitotic entry

Cell Division, 2010
The kinase Wee1 has been recognized for a quarter century as a key inhibitor of Cyclin dependent kinase 1 (Cdk1) and mitotic entry in eukaryotes. Nonetheless, Wee1 regulation is not well understood and its large amino-terminal regulatory domain (NRD) has
Enders Greg H
doaj   +1 more source

Maternal sevoflurane exposure induces neurotoxicity in offspring rats via the CB1R/CDK5/p-tau pathway

Frontiers in Pharmacology, 2023
Sevoflurane is widely used for maternal anesthesia during pregnancy. Sevoflurane exposure of rats at mid-gestation can cause abnormal development of the central nervous system in their offspring.
Yuxiao Wan, Ziyi Wu, Xingyue Li, Ping Zhao   +3 more
doaj   +1 more source