Results 111 to 120 of about 1,955 (149)

Screening for protective antigens of Cyprinid herpesvirus 2 and construction of DNA vaccines

Journal of Virological Methods, 2020
In recent years, crucian carp hematopoietic necrosis caused by Cyprinid herpesvirus 2 (CyHV-2) infection has caused an enormous economic loss to the aquaculture industry.In this study antigenic epitope analysis was performed on the membrane proteins of CyHV-2, and 8 antigen-rich peptide fragments were selected for prokaryotic expression.
Xuemei, Yuan, Jinyu, Shen, Xiaoyi, Pan, Jiayun, Yao, Sunjian, Lyu, Li, Liu, Haiqing, Zhang   +6 more
openaire   +2 more sources

Susceptibility of Japanese Cyprininae fish species to cyprinid herpesvirus 2 (CyHV-2)

Veterinary Microbiology, 2014
Cyprinid herpesvirus 2 (CyHV-2) is known as the causative agent of herpesviral haematopoietic necrosis (HVHN) of goldfish (Carassius auratus). Recently, the virus has also been detected from Prussian carp (C. gibelio) and crucian carp (C. carassius) from European and Asian countries.
Takafumi, Ito, Yukio, Maeno
openaire   +2 more sources

Proteomic and Phosphoproteomic Analyses Reveal Gibel Carp Responses to Cyprinid Herpesvirus 2 Infection

Journal of Proteome Research, 2022
Cyprinid herpesvirus 2 (CyHV-2) is a typical linear double-stranded DNA virus, which can induce severe herpesviral hematopoietic necrosis disease (HVHND) in gibel carp. However, the CyHV-2 infection mechanisms still remain unresolved till now. Here, we combined the isobaric tag for relative absolute quantitation (iTRAQ)-labeled quantitative proteomic ...
Chen Xu, Yiqun Li, Zidong Xiao, Jie Yang, Mingyang Xue, Nan Jiang, Yan Meng, Wenzhi Liu, Yuding Fan, Yong Zhou   +9 more
openaire   +2 more sources

Primary Establishment of LAMP Method for Cyprinid Herpesvirus 2 Detection

Applied Mechanics and Materials, 2014
A new loop-mediated isothermal amplification (LAMP) method for the detection of Cyprinid herpesvirus 2 (CyHV-2) was developed. Following the synthesis in vitro of a 413 bp DNA fragment of CyHV2 DNA-dependent DNA polymerase gene by overlap extension PCR using 11 overlapping oligo primers designed according to the gensome sequences information of CyHV2 ...
Ze Xiao Yang, Gui Li Li, Yin Wang, Xue Ping Yao, Kai Yu Wang, Shan Zhen Peng, Bo Wang, Hou Xun Ya, Ran Yang Ren   +8 more
openaire   +1 more source

In vitro characteristics of cyprinid herpesvirus 2: effect of kidney extract supplementation on growth

Diseases of Aquatic Organisms, 2015
Herpesviral hematopoietic necrosis caused by goldfish hematopoietic necrosis virus (now identified as cyprinid herpesvirus 2, CyHV-2) has contributed to economic losses in goldfish Carassius auratus culture and is becoming a major obstacle in Prussian carp C. gibelio aquaculture in China.
Tomoya, Shibata, Azusa, Nanjo, Masato, Saito, Keisuke, Yoshii, Takafumi, Ito, Teruyuki, Nakanishi, Takashi, Sakamoto, Motohiko, Sano   +7 more
openaire   +2 more sources

Development of a Polymerase Chain Reaction Assay to Detect Cyprinid Herpesvirus 2 in Goldfish

Journal of Aquatic Animal Health, 2009
Abstract Cyprinid herpesvirus 2 (CyHV2) has been associated with epidemic mortality among cultured populations of goldfish Carassius auratus. As the principal target tissues are hematopoietic cells in the kidney and spleen, the disease is designated herpesviral hematopoietic necrosis (HVHN).
Thomas B, Waltzek, Tomofumi, Kurobe, Andrew E, Goodwin, Ronald P, Hedrick   +3 more
openaire   +2 more sources

Application of a monoclonal antibody specific for the ORF92 capsid protein of Cyprinid herpesvirus 2

Journal of Virological Methods, 2018
Cyprinid herpesvirus 2 (CyHV-2) infection is a recently discovered viral disease of farmed silver crucian carp (Carassius auratus gibelio) in mainland China that has resulted in massive fish mortality and appears to be spreading cross the world. Various detection methods based on nucleic acids have been developed, but effective immunological diagnostic
Zhaoyuan Shen, Yousheng Jiang, Jianfei Lu, Motohiko Sano, Dan Xu, Liqun Lu   +5 more
openaire   +2 more sources

Identification and characterization of a tumor necrosis factor receptor like protein encoded by Cyprinid Herpesvirus 2

Developmental & Comparative Immunology, 2021
Virus-encoded tumor necrosis factor receptors (vTNFRs) facilitate viral escape from the host immune response during viral propagation. Cyprinid Herpesvirus-2 (CyHV-2) is a double-stranded DNA virus of alloherpesviridae family that causes great economic losses in the aquaculture industry.
Jian-Fei Lu, Tian-Cheng Jin, Ting Zhou, Xin-Jiang Lu, Jian-Ping Chen, Jiong Chen   +5 more
openaire   +2 more sources

Isolation of a cyprinid herpesvirus 2 from goldfish, Carassius auratus (L.), in the UK

Journal of Fish Diseases, 2007
AbstractHaematopoietic necrosis virus [cyprinid herpesvirus 2 (CyHV‐2)] was isolated during disease outbreaks in goldfish, Carassius auratus, at an ornamental fish retail site in southern England in 2004. Signs of disease included lethargy and inappetence and were first seen after water temperatures increased from 14–15 to 19–21 °C.
K R, Jeffery, K, Bateman, A, Bayley, S W, Feist, J, Hulland, C, Longshaw, D, Stone, G, Woolford, K, Way   +8 more
openaire   +2 more sources

Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of Cyprinid herpesvirus 2

Journal of Virological Methods, 2013
Goldfish Haematopoietic Necrosis caused by Cyprinid herpesvirus 2 (CyHV-2) is a severe fish disease with high level of mortality. This is the first study on detection of this disease by loop-mediated isothermal amplification (LAMP). A set of six primers targeting terminase gene (accession no.
Junqiang, He, Xiujie, Shi, Li, Yu, Xiaocong, Zheng, Wensheng, Lan, Peng, Jia, Jinjin, Wang, Hong, Liu   +7 more
openaire   +2 more sources