Results 261 to 270 of about 654,528 (315)
Some of the next articles are maybe not open access.
Current Protocols in Protein Science, 2017
AbstractThis unit describes a number of methods for modifying cysteine residues of proteins and peptides. A general procedure for alkylation of cysteine residues in a protein of known size and composition with haloacyl reagents or N‐ethylmaleimide (NEM) is presented, and alternate protocols describe similar procedures for use when the size and ...
openaire +2 more sources
AbstractThis unit describes a number of methods for modifying cysteine residues of proteins and peptides. A general procedure for alkylation of cysteine residues in a protein of known size and composition with haloacyl reagents or N‐ethylmaleimide (NEM) is presented, and alternate protocols describe similar procedures for use when the size and ...
openaire +2 more sources
Analytical Biochemistry, 1963
Abstract A technically easy, sensitive method for specific determination of cysteine was developed. It was based upon the fact that an equimolecular reaction between cysteine and noradrenochrome caused the pink color of the latter to change to yellow, while the pink color remained unchanged or merely faded in the presence of other sulfhydryl ...
openaire +2 more sources
Abstract A technically easy, sensitive method for specific determination of cysteine was developed. It was based upon the fact that an equimolecular reaction between cysteine and noradrenochrome caused the pink color of the latter to change to yellow, while the pink color remained unchanged or merely faded in the presence of other sulfhydryl ...
openaire +2 more sources
Cysteine-S-sulphonate as an Intermediate in Microbial Synthesis of Cysteine
Nature, 1960THE synthesis of cysteine in micro-organisms from inorganic sulphur sources has been studied for some time, and the available evidence suggests that sulphate sulphur is reduced to the thiosulphate or sulphide state prior to its introduction into the carbon chain of cysteine1–3, although some investigators have postulated that the formation of sulphur ...
T, NAKAMURA, R, SATO
openaire +2 more sources
EcoSal Plus, 2008
The synthesis of L-cysteine from inorganic sulfur is the predominant mechanism by which reduced sulfur is incorporated into organic compounds. L-cysteineis used for protein and glutathione synthesis and serves as the primary source of reduced sulfur in L-methionine, lipoic acid, thiamin, coenzyme A (CoA), molybdopterin, and other organic ...
openaire +2 more sources
The synthesis of L-cysteine from inorganic sulfur is the predominant mechanism by which reduced sulfur is incorporated into organic compounds. L-cysteineis used for protein and glutathione synthesis and serves as the primary source of reduced sulfur in L-methionine, lipoic acid, thiamin, coenzyme A (CoA), molybdopterin, and other organic ...
openaire +2 more sources
The Effect of Dietary Cysteine Level on Cysteine Metabolism in Rats
The Journal of Nutrition, 1982Male, Sprague-Dawley rats were fed L-amino acid diets that contained 0.4% L methionine and either 0, 0.2% (control), or 2.6% L-cysteine (free base) for 5 or 20 days. Hepatic cysteine dioxygenase activity in rats fed 2.6% cysteine was 5 and 3-times as great as in pair-fed control rats at 5 and 20 days, respectively.
K M, Daniels, M H, Stipanuk
openaire +2 more sources
A cysteine-selective fluorescent probe for the cellular detection of cysteine
Biomaterials, 2012A series of coumarin fluorophores (1-3), each bearing a double bond conjugated quinoline unit that can undergo a Michael-type reaction with thiol-containing compounds, is reported. These systems, designed to provide so-called turn-on changes in fluorescence response when exposed to thiols, act as fluorescent chemical sensors for cysteine (Cys ...
Hyo Sung, Jung +8 more
openaire +2 more sources
Cysteine proteinases and metastasis
CANCER AND METASTASIS REVIEW, 1984Cysteine proteinases are a subclass of endopeptidases which require activation by thiol reagents. A tumor cysteine proteinase which appears to be related to lysosomal cathepsin B has been implicated in the ability of tumor cells to invade the extracellular matrix and to metastasize to secondary sites.
B F, Sloane, K V, Honn
openaire +2 more sources
Degradation of DNA by cysteine
Archives of Biochemistry and Biophysics, 1971Abstract Exposure of DNA solutions to cysteine resulted in degradation of the polydeoxynucleotide. This reaction was inhibited by Na2 EDTA and by sodium citrate. Methylcysteine, mercaptoethanol, serine, and homocysteine did not exhibit this degradative effect. Preincubation of cysteine resulted in a more rapid rate of degradation thus suggesting that
H S, Rosenkranz, S, Rosenkranz
openaire +2 more sources
Biochemical Pharmacology, 1993
In this study, we have used a rat lung slice model to compare the ability to several potential cysteine delivery systems (L-cysteine isopropylester, L-cysteine cyclohexylester, N-acetylcysteine, L,2-oxo-4-thiazolidine carboxylic acid and cysteine) to elevate cysteine and glutathione (GSH) levels in control lung slices and slices depleted of their GSH ...
M, Butterworth +3 more
openaire +2 more sources
In this study, we have used a rat lung slice model to compare the ability to several potential cysteine delivery systems (L-cysteine isopropylester, L-cysteine cyclohexylester, N-acetylcysteine, L,2-oxo-4-thiazolidine carboxylic acid and cysteine) to elevate cysteine and glutathione (GSH) levels in control lung slices and slices depleted of their GSH ...
M, Butterworth +3 more
openaire +2 more sources
Cysteine Synthase: A Key Enzyme of Cysteine Biosynthetic Pathway
Biochemistry (Moscow)Cysteine is an amino acid essential for normal functioning of living organisms. In bacteria and plants, the main mechanism of cysteine synthesis is the thiolation pathway, the second stage of which is catalyzed by either cysteine synthase A (CysK), if the substrate is inorganic sulfide, or cysteine synthase B (CysM), if the substrate is thiosulfate ...
Evgenii K, Les +4 more
openaire +2 more sources