In-silico assessment of protein-protein electron transfer. a case study: cytochrome c peroxidase--cytochrome c. [PDF]
PLoS Computational Biology, 2013 The fast development of software and hardware is notably helping in closing the gap between macroscopic and microscopic data. Using a novel theoretical strategy combining molecular dynamics simulations, conformational clustering, ab-initio quantum ...
Frank H Wallrapp +2 more
doaj +3 more sources
Evolutionary Approaches to Study Cytochrome c Peroxidase
CHIMIA, 2001 Directed molecular evolution of enzymes and proteins has emerged as an extremely powerful method to create proteins with novel properties, both for practical applications as well as for mechanistic studies.
André Iffland +3 more
doaj +6 more sources
Cytochrome c-peroxidase modulates ROS homeostasis to regulate the sexual mating of Sporisorium scitamineum [PDF]
Microbiology Spectrum, 2023 Sugarcane smut, caused by the basidiomycetous fungus Sporisorium scitamineum, is a global fungal disease resulting in substantial economic losses. Our previous research has highlighted the significant role of reactive oxide species (ROS) in the sexual ...
Enping Cai +7 more
doaj +2 more sources
How Does Replacement of the Axial Histidine Ligand in Cytochrome <i>c</i> Peroxidase by N<sub>δ</sub>-Methyl Histidine Affect Its Properties and Functions? A Computational Study. [PDF]
Int J Mol Sci, 2020 Heme peroxidases have important functions in nature related to the detoxification of H2O2. They generally undergo a catalytic cycle where, in the first stage, the iron(III)–heme–H2O2 complex is converted into an iron(IV)–oxo–heme cation radical species ...
Lee CWZ +3 more
europepmc +2 more sources
Constraints on the Radical Cation Center of Cytochrome c Peroxidase for Electron Transfer from Cytochrome c. [PDF]
Biochemistry, 2016 The tryptophan 191 cation radical of cytochrome c peroxidase (CcP) compound I (Cpd I) mediates long-range electron transfer (ET) to cytochrome c (Cc). Here we test the effects of chemical substitution at position 191.
Payne TM, Yee EF, Dzikovski B, Crane BR.
europepmc +2 more sources
Enhancing Mn(II)-Binding and Manganese Peroxidase Activity in a Designed Cytochrome c Peroxidase through Fine-Tuning Secondary-Sphere Interactions. [PDF]
Biochemistry, 2016 Noncovalent second-shell interactions are important in controlling metal-binding affinity and activity in metalloenzymes, but fine-tuning these interactions in designed metalloenzymes has not been fully explored. As a result, most designed metalloenzymes
Hosseinzadeh P +7 more
europepmc +2 more sources
Two-dimensional infrared spectroscopic study of cytochrome c peroxidase activity in deep eutectic solvent [PDF]
Structural Dynamics, 2019 Deep eutectic solvents (DESs) prepared by mixing hydrogen-bond donor and acceptor molecules have been found to be of use in several applications. Recently, it was shown that DESs can enhance the peroxidation activity of cytochrome c.
Koji Osawa +4 more
doaj +2 more sources
Structure of the Zymomonas mobilis respiratory chain: oxygen affinity of electron transport and the role of cytochrome c peroxidase. [PDF]
Microbiology (Reading), 2014 The genome of the ethanol-producing bacterium Zymomonas mobilis encodes a bd-type terminal oxidase, cytochrome bc1 complex and several c-type cytochromes, yet lacks sequences homologous to any of the known bacterial cytochrome c oxidase genes.
Balodite E +6 more
europepmc +2 more sources
Identifying the elusive sites of tyrosyl radicals in cytochrome c peroxidase: implications for oxidation of substrates bound at a site remote from the heme. [PDF]
Biochemistry, 2014 The location of the Trp radical and the catalytic function of the [Fe(IV)=O Trp191•+] intermediate in cytochrome c peroxidase (CcP) are well-established; however, the unambiguous identification of the site(s) for the formation of tyrosyl radical(s) and ...
Miner KD +7 more
europepmc +2 more sources
In Silico/In Vivo Insights into the Functional and Evolutionary Pathway of Pseudomonas aeruginosa Oleate-Diol Synthase. Discovery of a New Bacterial Di-Heme Cytochrome C Peroxidase Subfamily. [PDF]
PLoS One, 2015 As previously reported, P. aeruginosa genes PA2077 and PA2078 code for 10S-DOX (10S-Dioxygenase) and 7,10-DS (7,10-Diol Synthase) enzymes involved in long-chain fatty acid oxygenation through the recently described oleate-diol synthase pathway.
Estupiñán M +4 more
europepmc +2 more sources