Results 141 to 150 of about 8,263 (168)

CyTOF: An Emerging Technology for Single‐Cell Proteomics in the Mouse

Current Protocols, 2021
AbstractThe ability to analyze the proteome of single cells is critical for the advancement of studies of steady‐state and pathological processes. Mass cytometry, or CyTOF, combines principles of mass spectrometry and flow cytometry to enable single‐cell analysis of protein expression.
Mónica Justice, Yeji An
exaly   +3 more sources

Immunometabolism the CyTOF way

Immunity, 2021
In this issue of Immunity, Levine et al. report a CyTOF-based approach for the analyses of CD8+ T cells metabolic changes at the single-cell level. This approach identified a transition state early in T cell activation that is characterized by high glycolytic and oxidative activity, providing new insight into the metabolic changes that underlie the ...
Liujia, Qian, Tiannan, Guo
openaire   +2 more sources

Validation of CyTOF Against Flow Cytometry for Immunological Studies and Monitoring of Human Cancer Clinical Trials [PDF]

Frontiers in Oncology, 2019
Flow cytometry is a widely applied approach for exploratory immune profiling and biomarker discovery in cancer and other diseases. However, flow cytometry is limited by the number of parameters that can be simultaneously analyzed, severely restricting ...
David Brooks, Lillian Siu, Cynthia Guidos   +2 more
exaly   +2 more sources

CIMAC-CIDC CyTOF harmonization.

Journal of Clinical Oncology, 2020
e15242 Background: The Cancer Immune Monitoring and Analysis Centers – Cancer Immunology Data Commons (CIMAC-CIDC) network is a National Cancer Institute-funded initiative to identify biomarkers of mechanisms and response to cancer immunotherapy clinical trials, using state-of-the-art assay technologies.
Adeeb Rahman, Bita Sahaf, Melanie Davila, Nicholas Fernandez, Emily McWilliams, Karen Millerchip, Salah E Bentebibel, Cara L. Haymaker, Natalia Sigal, Caroline Duault, Emily Thrash, Diane Del Valle, Beatriz Sanchez Espiridion, Mina Pichavant, Chantale Bernatchez, Ignacio Ivan Wistuba, Sacha Gnjatic, Sean Bendall, Holden Maecker   +18 more
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Parameter optimization for stable clustering using FlowSOM: a case study from CyTOF [PDF]

Frontiers in Immunology
High-dimensional cell phenotyping is a powerful tool to study molecular and cellular changes in health and diseases. CyTOF enables high-dimensional cell phenotyping using tens of surface and intra-cellular markers. To utilize the full potential of CyTOF,
Khadir Raddassi, Weiyang Tao, Aridaman Paṇḍit   +2 more
exaly   +2 more sources

Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype

2022
Regulation of hematopoiesis is dependent upon interactions between hematopoietic stem/progenitor cells and niche components, requiring a highly diverse array of different cell-cell interactions and cell signaling events. The overwhelming diversity of the components that can regulate hematopoiesis, especially when factoring in how the cell surface and ...
Safa F, Mohamad, Maegan L, Capitano
openaire   +2 more sources

An R-based reproducible and user-friendly preprocessing pipeline for CyTOF data [PDF]

F1000Research, 2020
Mass cytometry (CyTOF) has become a method of choice for in-depth characterization of tissue heterogeneity in health and disease, and is currently implemented in multiple clinical trials, where higher quality standards must be met. Currently, preprocessing of raw files is commonly performed in independent standalone tools, which makes it difficult to ...
Mark Robinson, Helena Crowell, Bernd Bodenmiller   +2 more
exaly   +4 more sources

CyTOF Mass Cytometry for Click Proliferation Assays

Current Protocols in Cytometry, 2017
AbstractNovel cell analyzers, including polychromatic flow cytometers and isotopical cytometry by time of flight (CyTOF) mass cytometers, enable simultaneous measurement of virtually bondless characteristics at the single‐cell level. BrdU assays for quantifying cellular proliferation are common but have several limitations, including the need for a DNA
Tosevski V, Ulashchik E, Trovato A, Cappella P   +3 more
openaire   +3 more sources

Graph Partitioning Algorithms and Applications on CyTOF Experiments

2023
Graph partitioning is a fundamental problem in computer science and engineering with diverse applications in fields such as circuit design, network analysis, and data mining [6]. The goal of graph partitioning is to divide a given graph into multiple clusters, with the aim of minimizing certain criteria, such as the number of edges between clusters ...
openaire   +1 more source

Tumor immune landscape paintings from the CyTOF period

Science Immunology, 2017
Early lung cancer patients harbor cancer-permissive innate immunity in the tumor microenvironment.
openaire   +2 more sources