Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF)
2022Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological ...
Tania, Tan +2 more
openaire +2 more sources
EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF
2018The ability to elucidate the phenotype of brain tumor initiating cell (BTIC) in the context of bulk tumor in glioblastoma multiforme (GBM) provides significant therapeutic benefits for therapeutic evaluation. For the identification of such an elusive and rare subpopulation of cells, a single cell analysis technology with deep profiling capabilities ...
Amy X, Hu +6 more
openaire +2 more sources
S128 CyTOF and in vitro analysis of the role of IL-17A in asthma
Advances in asthma science and treatment, 2019Introduction Many patients with asthma have type-2 low, neutrophilic asthma, and this has been linked to elevated IL-17A levels. We aimed to explore the role of IL-17A in asthma using two approaches: i) mass cytometry by time of flight (CyTOF) profiling of blood and sputum for IL-17A-expressing cells; and ii) in vitro modelling of the effects of IL ...
GM Hynes +12 more
openaire +1 more source
CyTOF Intracellular Cytokine Assays for Antigen-Specific T Cells
T cells specific for a single antigen tend to be rare, even after expansion of memory cells. They are commonly detected by in vitro stimulation with peptides or protein, followed by staining for intracellular cytokines. In this protocol, CyTOF® mass cytometry is used to collect single-cell data on a large number of cytokines/chemokines, as well as cell-Dongxia, Lin, Holden T, Maecker
openaire +2 more sources
A streamlined whole blood CyTOF workflow defines a circulating immune cell signature of COVID ‐19
Cytometry Part A: the Journal of the International Society for Analytical Cytology, 2021Assaf Magen +2 more
exaly
Regulatory T-Cell Phenotyping Using CyTOF
Methods in Molecular Biology, 2023Hugo Barcenilla +2 more
exaly
CyTOF workflow: Differential discovery in high-throughput high-dimensional cytometry datasets
F1000Research, 2017Mitchell Lévesque +2 more
exaly +2 more sources
CyTOF workflow: differential discovery in high-throughput high-dimensional cytometry datasets
F1000Research, 2019Mitchell Lévesque +2 more
exaly +2 more sources
An R-based reproducible and user-friendly preprocessing pipeline for CyTOF data
F1000Research, 2022Mark Robinson +2 more
exaly