Results 221 to 230 of about 13,763 (258)

Pore-Forming Bacterial Protein Hemolysins (Cytolysins)

Critical Reviews in Microbiology, 1991
Protein toxins forming pores in biological membranes occur frequently in Gram-positive and Gram-negative bacteria. They kill either bacteria or eukaryotic cells (at most, a few seem to act on both groups of organisms). Most of the toxins affecting eukaryotes have clearly been shown to be related to the pathogenicity of the producing organisms.
V, Braun, T, Focareta
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Preparation of Cytolysin A (ClyA) Nanopores

2020
The ionic currents passing through nanopores can be used to sequence DNA and identify molecules at the single-molecule level. Recently, researchers have started using nanopores for the detection and analysis of proteins, providing a new platform for single-molecule enzymology studies and more efficient biomolecular sensing applications.
Galenkamp, Nicole Stéphanie, Van Meervelt, Veerle, Mutter, Natalie Lisa, van der Heide, Nieck Jordy, Wloka, Carsten, Maglia, Giovanni   +5 more
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The Cholesterol-Dependent Cytolysins

2001
In view of the recent studies on the CDCs, a reasonable schematic of the stages leading to membrane insertion of the CDCs can be assembled. As shown in Fig. 3, we propose that the CDC first binds to the membrane as a monomer. These monomers then diffuse laterally on the membrane surface to encounter other monomers or incomplete oligomeric complexes ...
R K, Tweten, M W, Parker, A E, Johnson
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Molecular cloning of rat cytolysin.

The Journal of Immunology, 1989
Abstract Rat cytolysin is one of the cytolytic factors present in the cytoplasmic granules of rat NK-like cytolytic cells and purified cytolysin exhibits an apparent Mr or 70 kDa. Cytolysis produced by cytolysin occurs in the presence of Ca2+ and is accompanied by the formation of membrane lesions of 160 A diameter.
H, Ishikawa, Y, Shinkai, H, Yagita, C C, Yue, P A, Henkart, S, Sawada, H A, Young, C W, Reynolds, K, Okumura   +8 more
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Partial characterization of a Moraxella bovis cytolysin

Veterinary Microbiology, 1995
Moraxella bovis (M. bovis) is the etiologic agent of infectious bovine keratoconjunctivitis and M. bovis hemolysin is believed to be an important virulence factor. Two strains of M. bovis were compared, Epp 63(300) (Epp), a known virulent and hemolytic strain, and IBH 63 (IBH), a known avirulent and nonhemolytic strain.
J T, Gray, P J, Fedorka-Cray, D G, Rogers   +2 more
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Binding of cholesterol by sulfhydryl-activated cytolysins

Infection and Immunity, 1980
The binding of cholesterol by pneumolysin, alveolysin, and streptolysin O has been demonstrated. The properties of the cytolysin-cholesterol interaction parallel those of cytolysin-erythrocyte interaction in that the reaction is rapid, temperature independent, decreased at elevated pH, and shows the same specificity with respect to other related ...
M K, Johnson, C, Geoffroy, J E, Alouf
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Characterization of non-lytic cytolysin-membrane intermediates

Molecular Immunology, 1991
In order to understand the nature of cytolysin-membrane interactions, the characteristics of stable, non-lytic cytolysin-target cell intermediates formed at low ionic strength, neutral pH, and at physiological ionic strength, pH 6.0, were examined.
A E, Kuta, C L, Bashford, C A, Pasternak, C W, Reynolds, P A, Henkart   +4 more
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Sea anemone cytolysins as toxic components of immunotoxins

Toxicon, 2009
The use of membrane active toxins as toxic moieties in the construction of immunotoxins (ITs) is an attractive alternative to overcome some of the problems of classical ITs since these new conjugates are based in the use of a different mechanism of killing undesired cells.
Tejuca M, Anderluh G, Dalla Serra M
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Mechanism of lysis by large granular lymphocyte granule cytolysin: generation of a stable cytolysin-RBC intermediate.

The Journal of Immunology, 1989
Abstract The effect of ionic strength and pH on the hemolytic activity of large granular lymphocyte granule cytolysin was examined in detail. Cytolysin-mediated lysis of RBC was inhibited by either low ionic strength or low pH. Under these conditions a nonlytic cytolysin-RBC intermediate was formed as revealed by hemolysis when cytolysin
A E, Kuta, C R, Reynolds, P A, Henkart
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Measurement of Cytolysin Hemolytic Activity

1993
Determine the lytic functional activity of cytolysin (perform) in granule and cell extracts. Red cell lysis is measured by optical absorbance of supernatant hemoglobin.
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