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Mass Cytometry Imaging for the Study of Human Diseases—Applications and Data Analysis Strategies

open access: yesFrontiers in Immunology, 2019
High parameter imaging is an important tool in the life sciences for both discovery and healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) are two relatively recent technologies which enable clinical samples to ...
Heeva Baharlou   +9 more
doaj   +2 more sources

A compendium of single extracellular vesicle flow cytometry

open access: yesJournal of Extracellular Vesicles, 2023
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs.
J. Welsh   +23 more
semanticscholar   +1 more source

Flow Cytometry: The Next Revolution

open access: yesCells, 2023
Unmasking the subtleties of the immune system requires both a comprehensive knowledge base and the ability to interrogate that system with intimate sensitivity.
J. Paul Robinson   +4 more
semanticscholar   +1 more source

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

open access: yesEuropean Journal of Immunology, 2021
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell ...
A. Cossarizza   +192 more
semanticscholar   +1 more source

Flow cytometry: past and future.

open access: yesBioTechniques, 2022
Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in many areas of science, in particular biotechnology and medicine, but also in industrial ...
J. Paul Robinson
semanticscholar   +1 more source

Cytometry and genetics [PDF]

open access: yesCytometry Part A, 2004
FLOW CYTOGENETICS Flow cytometric analyses of individual chromosomes were made possible by the development of techniques for isolating intact chromosomes (1) and by procedures for staining chromosomes with DNA-specific fluorescent dyes. The term flow cytogenetics (2) was coined in the early 1980s to describe the application of flow cytometry and ...
Joe W. Gray   +2 more
openaire   +3 more sources

cyCombine allows for robust integration of single-cell cytometry datasets within and across technologies

open access: yesNature Communications, 2022
Combining single-cell cytometry datasets increases the analytical flexibility and the statistical power of data analyses. However, in many cases the full potential of co-analyses is not reached due to technical variance between data from different ...
C. B. Pedersen   +11 more
semanticscholar   +1 more source

Flow Cytometry II: Mass and Imaging Cytometry [PDF]

open access: yesJournal of Investigative Dermatology, 2015
Correction to: Journal of Investigative Dermatology (2015) 135, e36; doi:10.1038/id.2015 ...
Richard R. Jahan-Tigh   +3 more
openaire   +5 more sources

MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

open access: yesJournal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented ...
J. Welsh   +26 more
semanticscholar   +1 more source

Application of Machine Learning for Cytometry Data

open access: yesFrontiers in Immunology, 2022
Modern cytometry technologies present opportunities to profile the immune system at a single-cell resolution with more than 50 protein markers, and have been widely used in both research and clinical settings.
Zicheng Hu   +2 more
semanticscholar   +1 more source

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