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Mass Cytometry Imaging for the Study of Human Diseases—Applications and Data Analysis Strategies

open access: yesFrontiers in Immunology, 2019
High parameter imaging is an important tool in the life sciences for both discovery and healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) are two relatively recent technologies which enable clinical samples to ...
Heeva Baharlou   +9 more
doaj   +1 more source

Determining clinically relevant features in cytometry data using persistent homology [PDF]

open access: yes, 2022
Cytometry experiments yield high-dimensional point cloud data that is difficult to interpret manually. Boolean gating techniques coupled with comparisons of relative abundances of cellular subsets is the current standard for cytometry data analysis. However, this approach is unable to capture more subtle topological features hidden in data, especially ...
arxiv   +1 more source

A Novel, Five-Marker Alternative to CD16–CD14 Gating to Identify the Three Human Monocyte Subsets

open access: yesFrontiers in Immunology, 2019
Human primary monocytes are heterogeneous in terms of phenotype and function, but are sub-divided only based on CD16 and CD14 expression. CD16 expression distinguishes a subset of monocytes with highly pro-inflammatory properties from non-CD16 expressing
Siew-Min Ong   +12 more
doaj   +1 more source

Calibration-Aimed Comparison of Image-Cytometry- and Flow-Cytometry-Based Approaches of Ploidy Analysis

open access: yesSensors, 2022
Ploidy analysis is the fundamental method of measuring DNA content. For decades, the principal way of conducting ploidy analysis was through flow cytometry. A flow cytometer is a specialized tool for analyzing cells in a solution.
Viktor Zoltán Jónás   +3 more
doaj   +1 more source

Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance

open access: yesSensors, 2019
This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization
Julien Claudel   +3 more
doaj   +1 more source

Genetic Characterization of a Plum Landrace Collection from La Palma, Canary Islands

open access: yesAgronomy, 2022
A plum collection located in the island of La Palma, Canary Islands, composed of twenty-nine European and Japanese plums was analyzed using nine simple sequence repeat (SSR) highly polymorphic loci.
Verónica Pérez   +4 more
doaj   +1 more source

Fluctuation of Information Entropy Measures in Cell Image

open access: yesEntropy, 2017
A simple, label-free cytometry technique is introduced. It is based on the analysis of the fluctuation of image Gray Level Information Entropy (GLIE) which is shown to reflect intracellular biophysical properties like generalized entropy.
Ishay Wohl   +4 more
doaj   +1 more source

Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy

open access: yesApplied Sciences, 2018
To understand complex biological processes, scientists must gain insight into the function of individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the cell’s life is retrieved, live-cell imaging allows ...
Zahra El-Schich   +2 more
doaj   +1 more source

Platelet surface marker analysis by mass cytometry

open access: yesPlatelets, 2020
Mass cytometry is a next generation flow cytometry technology which analyzes cells one at a time (up to 1000/sec) using mass spectrometry to detect probes labeled with rare-earth metals.
Thomas A. Blair, Andrew L. Frelinger
doaj   +1 more source

Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

open access: yesBiosensors, 2018
SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of ...
Richard B. M. Schasfoort   +4 more
doaj   +1 more source

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