Results 51 to 60 of about 937,182 (344)

Investigation of Ethidium Bromide Interaction with Yersinia Bacterial Cells in Lyophilized/Rehydrated Preparations

Проблемы особо опасных инфекций, 2012
Investigated was ethidium bromide interaction with the cells of avirulent Y. pestis and Y. pseudotuberculosis strains in lyophilized/rehydrated preparations.
V. A. Govorunova, I. G. Govorunov, V. V. Firstova, E. V. Zyrina   +3 more
doaj   +1 more source

Novel and unscrutinized immune entities of the zebrafish gut

FEBS Letters, EarlyView.
Understudied cells of the zebrafish immune system include bona fide immune cells and epithelial (‐derived) cells with immune functions. Research focusing on zebrafish cells which demonstrate similarities to mammalian immune cell counterparts may help us understand the pathologies in which they are implicated. Currently available and advanced tools make
Audrey Inge Schytz Andersen‐Civil, Marine Mantel, Julia Soh, Serge Mostowy, Sylvia Brugman, Gilles Claude Vanwalleghem   +5 more
wiley   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Virtual-freezing fluorescence imaging flow cytometry

Nature Communications, 2020
By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, and ...
H. Mikami, Makoto Kawaguchi, Chun-Jung Huang, Hiroki Matsumura, Takeaki Sugimura, Kangrui Huang, Cheng Lei, Shunnosuke Ueno, Taichi Miura, Takuro Ito, K. Nagasawa, T. Maeno, H. Watarai, M. Yamagishi, S. Uemura, S. Ohnuki, Y. Ohya, Hiromi Kurokawa, S. Matsusaka, Chia‐Wei Sun, Y. Ozeki, K. Goda   +21 more
semanticscholar   +1 more source

Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers

FEBS Letters, EarlyView.
Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur, Brian Acquaviva, Baylee A. Smith, Nathan Walker, Isabella Walter, Matthew L. Wohlever   +5 more
wiley   +1 more source

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

Unique gating strategy identifier based on the Prime Population System and Gödel Numbers

Frontiers in Immunology
Gating is a fundamental and essential process in cytometry data analysis since it defines cell types of interest. Currently, there is no universally accepted method for representing and sharing gating strategies among software, publications, and ...
Antonio Cosma
doaj   +1 more source

Tonic signaling of the B‐cell antigen‐specific receptor is a common functional hallmark in chronic lymphocytic leukemia cell phosphoproteomes at early disease stages

Molecular Oncology, EarlyView.
B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez, Pablo Juanes‐Velasco, Marina L. García‐Vaquero, Conrad Droste, Alicia Landeira‐Viñuela, Miguel Alcoceba, Helena Fidalgo‐Gómez, Sara Misiego‐Herrero, Almudena Navarro‐Bailón, Mónica Baile, José M. Bastida, Jose Manuel Sanchez‐Santos, Rafael Góngora, Julia Almeida, Marcos Gonzalez‐Diaz, Alberto Orfao, Javier De Las Rivas, Manuel Fuentes   +17 more
wiley   +1 more source

Improving oligo-conjugated antibody signal in multimodal single-cell analysis

eLife, 2021
Simultaneous measurement of surface proteins and gene expression within single cells using oligo-conjugated antibodies offers high-resolution snapshots of complex cell populations.
Terkild B Buus, Alberto Herrera, Ellie Ivanova, Eleni Mimitou, Anthony Cheng, Ramin S Herati, Thales Papagiannakopoulos, Peter Smibert, Niels Odum, Sergei B Koralov   +9 more
doaj   +1 more source

The Martians of cytometry? [PDF]

Cytometry Part A, 2008
the first half of the last century a group of five Hungarianphysicists and mathematicians were termed the Martians ofScience (1). They were Edward Teller, Leo Szilard, John (von)Neumann, Eugene P. Wigner, Theodore von Karman. Thesefive really changed the world when working in Los Alamosback in the 1930’s.
openaire   +3 more sources