Results 51 to 60 of about 1,097,210 (332)

Method for Isolation of Myxozoan Proliferative Stages from Fish at High Yield and Purity: An Essential Prerequisite for In Vitro, In Vivo and Genomics-Based Research Developments

Cells, 2022
Myxozoans are a diverse group of microscopic cnidarian parasites and some representatives are associated with important diseases in fish, in both marine and freshwater aquaculture systems.
Ana Born-Torrijos, Anush Kosakyan, Sneha Patra, Joana Pimentel-Santos, Brian Panicucci, Justin Tze Ho Chan, Tomáš Korytář, Astrid S. Holzer   +7 more
doaj   +1 more source

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

Droplet Microfluidic Technology for the Early and Label-Free Isolation of Highly-Glycolytic, Activated T-Cells

Micromachines, 2022
A label-free, fixation-free and passive sorting method is presented to isolate activated T-cells shortly after activation and prior to the display of activation surface markers. It uses a recently developed sorting platform dubbed “Sorting by Interfacial
Claudia Zielke, Adriana J. Gutierrez Ramirez, Kelsey Voss, Maya S. Ryan, Azam Gholizadeh, Jeffrey C. Rathmell, Paul Abbyad   +6 more
doaj   +1 more source

Joint Modeling and Registration of Cell Populations in Cohorts of High-Dimensional Flow Cytometric Data [PDF]

, 2013
In systems biomedicine, an experimenter encounters different potential sources of variation in data such as individual samples, multiple experimental conditions, and multi-variable network-level responses.
Duong, Tarn, Hafler, David, Irish, Jonathan, Lee, Sharon, Levy, Ronald, McLachlan, Geoffrey J., Mesirov, Jill, Nazaire, Marc-Danie, Ng, Shu-Kay, Nolan, Garry, Pyne, Saumyadipta, Tamayo, Pablo, Wang, Kui   +12 more
core   +4 more sources

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

The detrimental impact of seminal plasma on the quality of cryopreserved sperm in goat bucks: freezing–thawing as the most harmful step

Frontiers in Veterinary Science
IntroductionSeminal plasma (SP) from goat bucks must be removed before freezing for obtaining sperm surviving the cryopreservation process if egg yolk-or skimmed milk (SM)-based extenders are used.MethodsWe studied the effect of SP on goat sperm quality ...
Inés Carolina Esteve, Inés Carolina Esteve, María Lorena Mocé, María Lorena Mocé, Ernesto Angel Gómez, Ernesto Angel Gómez, Rosario Martín-Montalbán, Andrea Martínez-Climent, Eva Mocé, Eva Mocé   +9 more
doaj   +1 more source

Rational Design and Synthesis of Large Stokes Shift 2,6-Sulphur-Disubstituted BODIPYs for Cell Imaging

Chemosensors, 2022
Five new disubstituted 2,6-thioaryl-BODIPY dyes were synthesized via selective aromatic electrophilic substitution from commercially available thiophenols.
Abigail E. Reese, Charles Lochenie, Ailsa Geddis, Luana A. Machado, Marcos C. de Souza, Flávia F. C. Marques, Carlos A. de Simone, Marcos M. Gouvêa, Leandro F. Pedrosa, Eufrânio N. da Silva Júnior, Marc Vendrell   +10 more
doaj   +1 more source

ROR1 Flow Cytometry [PDF]

BIO-PROTOCOL, 2013
ROR1 is a receptor tyrosine kinase family member studied for its roles in development and cancer. Here we describe a protocol for analysis of ROR1 surface expression in acute lymphoblastic leukemia immortalized cell lines by flow cytometry.
Vincent Bicocca, Jeffrey Tyner
openaire   +3 more sources

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Extremely High-Throughput Parallel Microfluidic Vortex-Actuated Cell Sorting

Micromachines, 2021
We demonstrate extremely high-throughput microfluidic cell sorting by making a parallel version of the vortex-actuated cell sorter (VACS). The set-up includes a parallel microfluidic sorter chip and parallel cytometry instrumentation: optics, electronics
Alex A. Zhukov, Robyn H. Pritchard, Mick J. Withers, Tony Hailes, Richard D. Gold, Calum Hayes, Mette F. la Cour, Fred Hussein, Salman Samson Rogers   +8 more
doaj   +1 more source