Developmental Cell, 2021 Cytoplasm is a gel-like crowded environment composed of various macromolecules, organelles, cytoskeletal networks, and cytosol. The structure of the cytoplasm is highly organized and heterogeneous due to the crowding of its constituents and their effective compartmentalization.
Silvia Caballero-Mancebo +2 more
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Androgen receptor phosphorylation at serine 515 by Cdk1 predicts biochemical relapse in prostate cancer patients [PDF]
, 2013 <br>Background:Prostate cancer cell growth is dependent upon androgen receptor (AR) activation, which is regulated by specific kinases. The aim of the current study is to establish if AR phosphorylation by Cdk1 or ERK1/2 is of prognostic ...
BV Kallakury +40 more
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DJ-1 as a deglycating enzyme: A unique function to explain a multifaceted protein? [PDF]
, 2017 The recently reported deglycating activity of DJ-1 reconciles several features previously described for the protein. The deglycating activity reported for DJ-1 may explain its different subcellular localizations, indeed the protein has been found in the ...
Bisaglia, Marco, DE LAZZARI, Federica
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Pre-implantation mouse embryos cultured In vitro under different oxygen concentrations show altered ultrastructures [PDF]
, 2020 Assisted Reproductive Technologies routinely utilize different culture media and oxygen (O2) concentrations to culture human embryos. Overall, embryos cultured under physiological O2 tension (5%) have improved development compared to embryos cultured ...
Antonouli, S +6 more
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Formation of viable cell fragments by treatment with colchicine [PDF]
, 1976 Time-lapse cinematography of human fibroblasts revealed that mitotic cells separated into numerous cell fragments containing varying amounts of chromatin and cytoplasm when treated with colchicine.
Abercrombie +18 more
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Additivities for Soluble Recombinant Protein Expression in Cytoplasm of Escherichia coli
FermentationRecombinant protein expression in Escherichia coli is a fundamental technique in molecular biology and biotechnology. This review provides a comprehensive overview of various additivities to enhance the expression levels of soluble recombinant proteins ...
Denis L. Atroshenko +3 more
doaj +1 more source
Folding and cytoplasm viscoelasticity contribute jointly to chromosome dynamics
, 2017 The chromosome is a key player of cell physiology, and its dynamics provides valuable information about its physical organization. In both prokaryotes and eukaryotes, the short-time motion of chromosomal loci has been described as a Rouse model in a ...
Cosentino-Lagomarsino, M. +3 more
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Mad2, Bub3, and Mps1 regulate chromosome segregation and mitotic synchrony in Giardia intestinalis, a binucleate protist lacking an anaphase-promoting complex. [PDF]
, 2014 The binucleate pathogen Giardia intestinalis is a highly divergent eukaryote with a semiopen mitosis, lacking an anaphase-promoting complex/cyclosome (APC/C) and many of the mitotic checkpoint complex (MCC) proteins.
Cande, W Zacheus, Vicente, Juan-Jesus
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Enzyme localization can drastically affect signal amplification in signal transduction pathways [PDF]
, 2005 Push-pull networks are ubiquitous in signal transduction pathways in both prokaryotic and eukaryotic cells. They allow cells to strongly amplify signals via the mechanism of zero-order ultrasensitivity.
van Albada, Siebe B. +1 more
core +5 more sources
Salting‐in the microbial cytoplasm [PDF]
FEBS Letters, 2006 Microbial organisms are known to rely for osmotic regulatory purposes on an assortment of low molecular weight molecules earmarked for function as osmolytes. The so‐called ‘compatible’ subclass of osmolyte, notably glycine betaine, is distinguished by a propensity to avoid the large bound fraction of cytoplasmic water adsorbed at the surface of ...
openaire +3 more sources