Results 21 to 30 of about 1,351 (97)

Construction of hyperthermostable d-allulose 3-epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus. [PDF]

FEBS Open Bio
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Shimada K, Ohtani K, Gullapalli PK, Ishikawa K.   +3 more
europepmc   +2 more sources

Safety of D-allulose as a novel food pursuant to Regulation (EU) 2015/2283. [PDF]

EFSA J
Abstract Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on D‐allulose as a novel food (NF) pursuant to Regulation (EU) 2015/2283. During the risk assessment, the Panel identified a number of data gaps, which needed to be addressed by the applicant ...
EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Turck D, Cámara M, Bohn T, Castenmiller J, De Henauw S, Jos Á, Maciuk A, Mangelsdorf I, McNulty B, Naska A, Pentieva K, Siani A, Thies F, Aguilera-Gómez M, Cubadda F, Frenzel T, Heinonen M, Knutsen HK, McArdle HJ, Neuhäuser-Berthold M, Poulsen M, Maradona MP, Schlatter JR, Siskos A, van Loveren H, Multari S, Rossi A, Hirsch-Ernst KI.   +29 more
europepmc   +2 more sources

Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose. [PDF]

Appl Environ Microbiol, 2006
Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK.
europepmc   +2 more sources

An Update: Enzymatic Synthesis for Industrial Applications. [PDF]

Angew Chem Int Ed Engl
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Bayer T, Wu S, Snajdrova R, Baldenius K, Bornscheuer UT.   +4 more
europepmc   +2 more sources

Reaction Engineering and Comparison of Electroenzymatic and Enzymatic ATP Regeneration Systems

ChemElectroChem, Volume 10, Issue 22, November 14, 2023., 2023
An electrochemically coupled ATP regeneration by pyruvate oxidase and acetate kinase for the phosphorylation of mevalonate was established and expanded by a polyphosphate kinase. The reaction was characterized and compared with other ATP regenerating systems in terms of the phosphate donor properties and biocatalytic metrics.
Regine Siedentop, Tobias Prenzel, Siegfried R. Waldvogel, Katrin Rosenthal, Stephan Lütz   +4 more
wiley   +1 more source

Food manufacturing processes and technical data used in the exposure assessment of food enzymes

EFSA Journal, Volume 21, Issue 7, July 2023., 2023
Abstract Food enzymes are used for technical purposes in the production of food ingredients or foods‐as‐consumed. In the European Union, the safety of a food enzyme is evaluated by EFSA on the basis of a technical dossier provided by an applicant. Dietary exposure is an integral part of the risk assessment of food enzymes.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Claude Lambré, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Marcel Mengelers, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk van Loveren, Laurence Vernis, Holger Zorn, Yrjö Roos, Kyriaki Apergi, Daniele Cavanna, Yi Liu, Francesco Pesce, Giulio di Piazza, Rita Ferreira de Sousa, Andrew Chesson   +25 more
wiley   +1 more source

Gene Cloning, Structural Prediction, and Prokaryotic Expression of Agrobacterium tumefaciens D-Psicose-3-Epimerase(根癌农杆菌D-阿洛酮糖-3-差向异构酶基因克隆、结构预测及原核表达) [PDF]

Shipin kexue jishu xuebao, 2019
D-Psicose can be converted from D-fructose catalyzed by D-psicose-3-epimerase. To realize the heterologous expression of D-psicose-3-epimerase (DPEase), the primers were designed to clone and isolate the gene.
ZHU Xingxing(朱星星), YANG Peizhou(杨培周), DU Mingrui(杜明睿), WU Yun(吴芸), JIANG Shaotong(姜绍通)   +4 more
doaj   +1 more source

Insights into the potential for mutualistic and harmful host–microbe interactions affecting brown alga freshwater acclimation

Molecular Ecology, Volume 32, Issue 3, Page 703-723, February 2023., 2023
Abstract Microbes can modify their hosts' stress tolerance, thus potentially enhancing their ecological range. An example of such interactions is Ectocarpus subulatus, one of the few freshwater‐tolerant brown algae. This tolerance is partially due to its (un)cultivated microbiome.
Hetty KleinJan, Clémence Frioux, Gianmaria Califano, Méziane Aite, Enora Fremy, Elham Karimi, Erwan Corre, Thomas Wichard, Anne Siegel, Catherine Boyen, Simon M. Dittami   +10 more
wiley   +1 more source

Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications

Biotechnology and Bioengineering, Volume 119, Issue 10, Page 2609-2638, October 2022., 2022
Abstract Over the past decade, nanotechnology has been developed and employed across various entities. Among the numerous nanostructured material types, enzyme‐incorporated nanomaterials have shown great potential in various fields, as an alternative to biologically derived as well as synthetically developed hybrid structures.
Shamini Anboo, Sie Yon Lau, Jibrail Kansedo, Pow‐Seng Yap, Tony Hadibarata, Jaison Jeevanandam, Azlina H. Kamaruddin   +6 more
wiley   +1 more source

Update of the list of QPS‐recommended microbiological agents intentionally added to food or feed as notified to EFSA 16: suitability of taxonomic units notified to EFSA until March 2022

EFSA Journal, Volume 20, Issue 7, July 2022., 2022
Abstract The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre‐evaluation of the safety of microorganisms, intended for use in the food or feed chains, to support the work of EFSA's Scientific Panels.
EFSA Panel on Biological Hazards (BIOHAZ), Kostas Koutsoumanis, Ana Allende, Avelino Alvarez‐Ordóñez, Declan Bolton, Sara Bover‐Cid, Marianne Chemaly, Robert Davies, Alessandra De Cesare, Friederike Hilbert, Roland Lindqvist, Maarten Nauta, Luisa Peixe, Giuseppe Ru, Marion Simmons, Panagiotis Skandamis, Elisabetta Suffredini, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Just Vlak, Fulvio Barizzone, Michaela Hempen, Sandra Correia, Lieve Herman   +28 more
wiley   +1 more source