Results 41 to 50 of about 13,466,281 (178)

Gene Cloning, Structural Prediction, and Prokaryotic Expression of Agrobacterium tumefaciens D-Psicose-3-Epimerase(根癌农杆菌D-阿洛酮糖-3-差向异构酶基因克隆、结构预测及原核表达) [PDF]

open access: yesShipin kexue jishu xuebao, 2019
D-Psicose can be converted from D-fructose catalyzed by D-psicose-3-epimerase. To realize the heterologous expression of D-psicose-3-epimerase (DPEase), the primers were designed to clone and isolate the gene.
ZHU Xingxing(朱星星)   +4 more
doaj   +1 more source

Construction of hyperthermostable d-allulose 3-epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus. [PDF]

open access: yesFEBS Open Bio
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Shimada K   +3 more
europepmc   +2 more sources

Biochemical analysis and the preliminary crystallographic characterization of d-tagatose 3-epimerase from Rhodobacter sphaeroides

open access: yesMicrobial Cell Factories, 2017
Background d-Tagatose 3-epimerase epimerizes d-fructose to yield d-psicose, which is a rare sugar that exists in small quantities in nature and is difficult to synthesize chemically.
Zhengliang Qi   +7 more
doaj   +1 more source

Sequential whole cell conversion process for production of D-psicose and D- mannitol from D-fructose [PDF]

open access: yes, 2018
Rare sugars, which exist only limited quantities naturally, have received considerable attention because of its various specific nutritional and biological functions.
Kim, Jae-Eun   +3 more
core   +1 more source

Construction and Process Optimization of Cell Factory for Allitol Synthesis with Multienzymatic Cascade System(多酶级联系统制备蒜糖醇的细胞工厂构建及工艺优化) [PDF]

open access: yesShipin kexue jishu xuebao, 2022
Allitol is generally used as a novel functional sweetener with great potential applications in food, medical care and other fields. Based on the Izumoring strategy, a multienzymatic cascade system involved glucose isomerase, D-psicose 3-epimerase and ...
ZHAO Jingyi(赵婧邑)   +5 more
doaj   +1 more source

Utilization of Maillard reaction in moist-dry-heating system to enhance physicochemical and antioxidative properties of dried whole longan fruit

open access: yesHeliyon, 2021
This research aimed to enhance the physicochemical and antioxidant properties of dried whole longan fruit using Maillard reaction or non-enzymatic glycosylation (glycation) in a moist-dry-heating system at 60 °C with approximately 75% relative humidity ...
Chalermkwan Somjai   +5 more
doaj   +1 more source

Improvement in the thermostability of D-psicose 3-epimerase from Agrobacterium tumefaciens by random and site-directed mutagenesis. [PDF]

open access: yesAppl Environ Microbiol, 2011
The S213C, I33L, and I33L S213C variants of d -psicose 3-epimerase from Agrobacterium tumefaciens , which were obtained by random and site-directed mutagenesis, displayed increases of 2.5, 5, and 7.5°C in ...
Choi JG, Ju YH, Yeom SJ, Oh DK.
europepmc   +2 more sources

Safety evaluation of the food enzyme d‐tagatose 3‐epimerase from the genetically modified Escherichia coli strain PS‐Sav‐001

open access: yesEFSA Journal, 2023
The food enzyme d‐tagatose 3‐epimerase (EC 5.1.3.31) is produced with the genetically modified Escherichia coli strain PS‐Sav‐001 by SAVANNA Ingredients GmbH. The genetic modifications do not give rise to safety concerns.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP)   +23 more
doaj   +1 more source

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