Results 71 to 80 of about 11,363,030 (180)

Reconstitution of Methionine Cycle With ATP Regeneration for Whole‐Cell Catalysis of Creatine Production in Engineered Escherichia coli

Microbial Biotechnology, Volume 18, Issue 5, May 2025.
In this study, we constructed a CR biosynthesis pathway in E. coli by introducing exogenous AGAT and GAMT. We integrated the reconstitution methionine cycle with a polyphosphate kinase‐based ATP regeneration system, achieving a CR titre of 5.27 g/L with a productivity of 0.22 g/L/h.
Yuhua Sheng, Yaokang Wu, Linpei Zhang, Xueqin Lv, Jianghua Li, Long Liu, Guocheng Du, Jian Chen, Yanfeng Liu   +8 more
wiley   +1 more source

Assessment of Metabolome Variation in Field‐Grown Lettuce in Context to Its Different Types and Soil Types as Analyzed via GC‐MS Analysis and Using of Chemometric Tools

eFood, Volume 6, Issue 2, April 2025.
Lettuce (Lactuca sativa L.) is one of the most important ready‐to‐eat vegetables widely consumed worldwide owing to its myriad nutritional and health benefits. A total of 111 peaks were identified via gas chromatography‐mass spectrometry. ABSTRACT Lettuce (Lactuca sativa L.) is one of the most important ready‐to‐eat vegetables widely consumed worldwide
Mostafa H. Baky, Sally E. Khaled, Mohamed R. Khalifa, Mohamed A. Farag   +3 more
wiley   +1 more source

Biosynthesis of rare hexoses using microorganisms and related enzymes

Beilstein Journal of Organic Chemistry, 2013
Rare sugars, referred to as monosaccharides and their derivatives that rarely exist in nature, can be applied in many areas ranging from foodstuffs to pharmaceutical and nutrition industry, or as starting materials for various natural products and drug ...
Zijie Li, Yahui Gao, Hideki Nakanishi, Xiaodong Gao, Li Cai   +4 more
doaj   +1 more source

Enzymatic Conversion of D-Psicose From D-Glucose by the Co-Expression of D-Psicose 3-Epimerase and Glucose Isomerase

, 2020
Escherichia coli was genetically modified for the production of D-psicose. A modified plasmid containing an extra copy of gene expression elements, including Ribosomal Binding Site (RBS), T7 promoter, multiple restriction enzyme cloning site, was ...
Chen, Xiaoyan
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Signalling and regulation of plant development by carbon/nitrogen balance

Physiologia Plantarum, Volume 177, Issue 2, March/April 2025.
Abstract The two most abundant macronutrients in plant cells are carbon (C) and nitrogen (N). Coordination of their cellular metabolism is a fundamental factor in guaranteeing the optimal growth and development of plants. N availability and assimilation profoundly affect plant gene expression and modulate root and stem architecture, thus affecting ...
Iris Fañanás‐Pueyo, Gerardo Carrera‐Castaño, Mónica Pernas, Luis Oñate‐Sánchez   +3 more
wiley   +1 more source

Application-Oriented Marine Isomerases in Biocatalysis

Marine Drugs, 2020
The class EC 5.xx, a group of enzymes that interconvert optical, geometric, or positional isomers are interesting biocatalysts for the synthesis of pharmaceuticals and pharmaceutical intermediates.
Antonio Trincone
doaj   +1 more source

From Sweetness to Mouthfeel: A Review on Overcoming Sensory Barriers in Sugar‐Free Beverages

International Journal of Food Science, Volume 2025, Issue 1, 2025.
Background Over recent decades, considerable research has focused on understanding sweet taste mechanisms and developing new noncarbohydrate sweeteners due to rising noncommunicable diseases. New regulations limiting high sugar levels in food and beverages have driven innovation towards reduced‐sugar and sugar‐free products, often using noncarbohydrate
Imogen Ramsey, Jing Liu, Jan Hendrik Swiegers, Olayide Oladokun, Chandra Mohan Chandrasekar   +4 more
wiley   +1 more source

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 21: Suitability of taxonomic units notified to EFSA until September 2024

EFSA Journal, Volume 23, Issue 1, January 2025.
Abstract The qualified presumption of safety (QPS) process was developed to provide a safety assessment approach for microorganisms intended for use in food or feed chains. In the period covered by this Statement, no new information was found that would change the status of previously recommended QPS taxonomic units (TUs).
EFSA Panel on Biological Hazards (BIOHAZ), Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panagiotis Skandamis, Elisabetta Suffredini, Marianne Chemaly, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Angela Botteon, Baptista Carolina, Barizzone Fulvio, Sandra Correia, Lieve Herman   +28 more
wiley   +1 more source

Exploiting Archaeal/Thermostable Enzymes in Synthetic Chemistry: Back to the Future?

ChemCatChem, Volume 16, Issue 21, November 11, 2024.
Thermostable and (hyper)thermophilic enzymes offer important opportunities for innovative, stereoselective and sustainable chemistry transformations. Many of these are archaeal enzymes that have evolved to operate under extreme conditions, including at elevated temperature.
Gaurav P. Kudalkar, Virendra K. Tiwari, David B. Berkowitz   +2 more
wiley   +1 more source

Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-keto-D-pentonates, D-fructose to 5-keto-D-fructose, and D-psicose to 5-keto-D-psicose [PDF]

, 2019
A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with Gluconobacter Thailandicus NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ ...
Yakushi, Toshiharu, Hours, Roque Alberto, Ano, Yoshitaka, Akakabe, Yoshihiko, Matsushita, Kazunobu, Adachi, Osao, Kataoka, Naoya   +6 more
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