Results 41 to 50 of about 138 (123)

Toxicological Assessment of D‐Allulose From a Novel One‐Step Fermentation Process Using Genetically Modified Escherichia coli: A 90‐Day Dietary Toxicity Study in Rats

Journal of Toxicology, Volume 2026, Issue 1, 2026.
D‐Allulose is a rare monosaccharide structurally similar to D‐fructose, characterized by low caloric content and relatively high sweetness. This study aimed to conduct a 90‐day oral toxicity test to systemically evaluate the potential toxicological effects of D‐allulose produced via a novel one‐step fermentation process using genetically engineered ...
Zinan Li, Yanmin Nie, Hongju Du, Wenjing Zhang, Shan Zheng, Wenshu Cong, Ying Feng, Guojun Li, Haiming Jing, Junyu Ning, Shan Gao, Phuping sucharitakul   +11 more
wiley   +1 more source

Production, purification, characterization, and safety evaluation of constructed recombinant D-psicose 3-epimerase

Microbial Cell Factories
Background D-psicose 3-epimerase (DPEase) is a potential catalytic enzyme for D-psicose production. D-psicose, also known as D-allulose, is a low-calorie sweetener that has gained considerable attention as a healthy alternative sweetener due to its ...
Nisit Watthanasakphuban, Pimsiriya Srila, Phitsanu Pinmanee, Charatda Punvittayagul, Nopphon Petchyam, Boontiwa Ninchan   +5 more
doaj   +1 more source

Crystal structure and substrate specificity of D-galactose-6-phosphate isomerase complexed with substrates. [PDF]

PLoS ONE, 2013
D-Galactose-6-phosphate isomerase from Lactobacillus rhamnosus (LacAB; EC 5.3.1.26), which is encoded by the tagatose-6-phosphate pathway gene cluster (lacABCD), catalyzes the isomerization of D-galactose-6-phosphate to D-tagatose-6-phosphate during ...
Woo-Suk Jung, Raushan Kumar Singh, Jung-Kul Lee, Cheol-Ho Pan   +3 more
doaj   +1 more source

Rational Design to Improve D-Psicose-3-epimerase Thermal Stability of Blautia wexlerae

Shipin gongye ke-ji
To develop a novel D-psicose-3-epimerase possessing higher thermal stability, an exploration was carried out on the genome of the intestinal probiotic Blautia wexlerae.
Kaixuan RAN, Mingqiang XUN, Kaixin LIN, Yu FU, Lili WANG, Chunxiao ZHANG, Hong YI   +6 more
doaj   +1 more source

α-D,l-Sorbose

IUCrData, 2018
The racemic title compound, C6H12O6, consisting of C-4 epimers of psicose, was crystallized from an aqueous solution of an equimolar mixture of d- and l-sorboses.
Hiroaki Taguchi, Kouta Sogo, Tomohiko Ishii, Akihide Yoshihara, Kazuhiro Fukada   +4 more
doaj   +1 more source

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, Volume 15, Issue 9, Page 1508-1519, September 2025.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

Update: Enzymatische Synthese für industrielle Anwendungen

Angewandte Chemie, Volume 137, Issue 27, July 1, 2025.
Die Biokatalyse hat sich zu einer nachhaltigen und wettbewerbsfähigen Alternative zur herkömmlichen chemischen Synthese entwickelt und ermöglicht die enzymbasierte Herstellung nicht nur von chemischen Grundstoffen, sondern auch von (nicht natürlichen) Aminosäuren, (seltenen) Zuckern und synthetischen Nukleotiden.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

An Update: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, Volume 64, Issue 27, July 1, 2025.
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Reconstitution of Methionine Cycle With ATP Regeneration for Whole‐Cell Catalysis of Creatine Production in Engineered Escherichia coli

Microbial Biotechnology, Volume 18, Issue 5, May 2025.
In this study, we constructed a CR biosynthesis pathway in E. coli by introducing exogenous AGAT and GAMT. We integrated the reconstitution methionine cycle with a polyphosphate kinase‐based ATP regeneration system, achieving a CR titre of 5.27 g/L with a productivity of 0.22 g/L/h.
Yuhua Sheng, Yaokang Wu, Linpei Zhang, Xueqin Lv, Jianghua Li, Long Liu, Guocheng Du, Jian Chen, Yanfeng Liu   +8 more
wiley   +1 more source

Crystal structure of 6-deoxy-α-l-psicofuranose

Acta Crystallographica Section E: Crystallographic Communications, 2015
The title compound, C6H12O5, was crystallized from an aqueous solution of 6-deoxy-l-psicose (6-deoxy-l-allulose, (3S,4S,5S)-1,3,4,5-tetrahydroxyhexan-2-one), and the molecule was confirmed as α-furanose with a 3T4 (or E4) conformation, which is a ...
Akihide Yoshihara, Tomohiko Ishii, Tatsuya Kamakura, Hiroaki Taguchi, Kazuhiro Fukada   +4 more
doaj   +1 more source