Results 21 to 30 of about 848 (145)

D-Allulose (D-Psicose) Biotransformation From Allitol by a Newly Found NAD(P)-Dependent Alcohol Dehydrogenase From Gluconobacter frateurii NBRC 3264 and the Enzyme Characterization [PDF]

Frontiers in Microbiology, 2022
The NAD(P)-dependent alcohol dehydrogenase (ADH) gene was cloned from Gluconobacter frateurii NBRC 3264 and expressed in Escherichia coli BL21 star (DE3). The expressed enzyme was purified and the characteristics were investigated.
Xin Wen, Huibin Lin, Yuhang Ning, Guangwen Liu, Yilin Ren, Can Li, Chengjia Zhang, Jianqun Lin, Xin Song, Jianqiang Lin   +9 more
doaj   +2 more sources

Improved Performance of D-Psicose 3-Epimerase by Immobilisation on Amino-Epoxide Support with Intense Multipoint Attachment [PDF]

Foods, 2021
D-allulose is an epimer of D-fructose at the C-3 position. With similar sweetness to sucrose and a low-calorie profile, D-allulose has been considered a promising functional sweetener.
Yifan Bu, Tao Zhang, Bo Jiang, Jingjing Chen   +3 more
doaj   +2 more sources

Production, purification, characterization, and safety evaluation of constructed recombinant D-psicose 3-epimerase [PDF]

Microbial Cell Factories
Background D-psicose 3-epimerase (DPEase) is a potential catalytic enzyme for D-psicose production. D-psicose, also known as D-allulose, is a low-calorie sweetener that has gained considerable attention as a healthy alternative sweetener due to its ...
Nisit Watthanasakphuban, Pimsiriya Srila, Phitsanu Pinmanee, Charatda Punvittayagul, Nopphon Petchyam, Boontiwa Ninchan   +5 more
doaj   +2 more sources

Awakening the natural capability of psicose production in Escherichia coli [PDF]

npj Science of Food, 2023
Due to the rampant rise in obesity and diabetes, consumers are desperately seeking for ways to reduce their sugar intake, but to date there are no options that are both accessible and without sacrifice of palatability.
Jayce E. Taylor, Dileep Sai Kumar Palur, Angela Zhang, Jake N. Gonzales, Augustine Arredondo, Timothy A. Coulther, Amiruddin Bin Johan Lechner, Elys P. Rodriguez, Oliver Fiehn, John Didzbalis, Justin B. Siegel, Shota Atsumi   +11 more
doaj   +2 more sources

Adaptive Steered Molecular Dynamics Combined With Protein Structure Networks Revealing the Mechanism of Y68I/G109P Mutations That Enhance the Catalytic Activity of D-psicose 3-Epimerase From Clostridium Bolteae [PDF]

Frontiers in Chemistry, 2018
The scarcity, richness, and other important physiological functions of D-psicose make it crucial to increase the yield of D-psicose. The production of D-psicose can be accomplished by D-psicose 3-epimerase (DPEase) from Clostridium bolteae (CbDPEase ...
Jingxuan Zhu, Yi Li, Jinzhi Wang, Zhengfei Yu, Ye Liu, Yi Tong, Weiwei Han   +6 more
doaj   +2 more sources

Intestinal absorption, organ distribution, and urinary excretion of the rare sugar D-psicose [PDF]

Drug Design, Development and Therapy, 2014
Ikuko Tsukamoto,1,* Akram Hossain,2,3,* Fuminori Yamaguchi,2 Yuko Hirata,2 Youyi Dong,2 Kazuyo Kamitori,2 Li Sui,2 Machiko Nonaka,2 Masaki Ueno,4 Kazuyuki Nishimoto,5 Hirofumi Suda,5 Kenji Morimoto,6 Tsuyoshi Shimonishi,7,† Madoka Saito,8 Tao Song,
Tsukamoto I, Hossain A, Yamaguchi F, Hirata Y, Dong Y, Kamitori K, Sui L, Nonaka M, Ueno M, Nishimoto K, Suda H, Morimoto K, Shimonishi T, Saito M, Song T, Konishi R, Tokuda M   +16 more
doaj   +2 more sources

Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704. [PDF]

PLoS ONE, 2013
The noncharacterized protein CLOSCI_02528 from Clostridium scindens ATCC 35704 was characterized as D-psicose 3-epimerase. The enzyme showed maximum activity at pH 7.5 and 60°C.
Wenli Zhang, Dan Fang, Qingchao Xing, Leon Zhou, Bo Jiang, Wanmeng Mu   +5 more
doaj   +2 more sources

In Silico Analysis and Development of the Secretory Expression of D-Psicose-3-Epimerase in Escherichia coli [PDF]

Microorganisms
D-psicose-3-epimerase (DPEase), a key enzyme for D-psicose production, has been successfully expressed in Escherichia coli with high yield.
Nisit Watthanasakphuban, Boontiwa Ninchan, Phitsanu Pinmanee, Kittipong Rattanaporn, Suttipun Keawsompong   +4 more
doaj   +2 more sources

Rare sugar D-psicose prevents progression and development of diabetes in T2DM model Otsuka Long-Evans Tokushima Fatty rats [PDF]

Drug Design, Development and Therapy, 2015
Akram Hossain,1,2 Fuminori Yamaguchi,1 Kayoko Hirose,1 Toru Matsunaga,3 Li Sui,1 Yuko Hirata,1 Chisato Noguchi,1 Ayako Katagi,1 Kazuyo Kamitori,1 Youyi Dong,1 Ikuko Tsukamoto,4 Masaaki Tokuda11Department of Cell Physiology, Faculty of Medicine, Kagawa ...
Hossain A, Yamaguchi F, Hirose K, Matsunaga T, Sui L, Hirata Y, Noguchi C, Katagi A, Kamitori K, Dong Y, Tsukamoto I, Tokuda M   +11 more
doaj   +2 more sources

Effects of monosaccharides including rare sugars on proliferation of Entamoeba histolytica trophozoites in vitro [PDF]

Frontiers in Molecular Biosciences, 2023
Entamoeba histolytica is a parasitic protozoan with roles in pathogenicity of intestinal amoebiasis. E. histolytica trophozoites lack functional mitochondria and their energy production depends mostly on glycolysis.
Kentaro Kato, Kentaro Kato, Mitsumasa Miura, Hiroshi Tachibana, Ikuko Tsukamoto   +4 more
doaj   +2 more sources