Results 271 to 280 of about 20,235 (287)
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Detection of Tomato Spotted Wilt Virus (TSWV) Infection in Plants Using DAS-ELISA and Dot-ELISA
2021Plant viruses cause severe damages to crop productions each year worldwide. To prevent the losses caused by plant viruses, it is necessary to develop specific and efficient diagnostic tools to detect viruses. Among the current virus detection techniques, serological detection methods are considered to be rapid, simple, sensitive, and high throughput ...
Shibo, Gao, Jianxiang, Wu
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Journal of Phytopathology, 1991
AbstractSingle and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of ...
Etienne, Lucien +2 more
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AbstractSingle and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of ...
Etienne, Lucien +2 more
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Comparison of DAS‐ELISA and qRT‐PCR for the detection of cucurbit viruses in seeds
Annals of Applied Biology, 2019AbstractThe importance of seeds as virus vehicles for long‐distance dissemination makes essential the availability of adequate methods of analysis to guarantee the quality of seed lots. To improve the repertoire of sensitive methods for seed diagnosis, we have developed quantitative real‐time RT‐PCR assays (RT‐qPCR) based on the TaqMan technology to ...
Covadonga Torre +3 more
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Journal of Phytopathology, 1996
AbstractIn order to develop a reliable diagnostic test for pepper veinal mottle virus (PVMV), a panel of monoclonal antibodies (mAbs), raised to a Nigerian isolate (PVMW‐Ni), was evaluated by double‐antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) on infected pepper samples from seven West African countries.
C. Huguenot +3 more
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AbstractIn order to develop a reliable diagnostic test for pepper veinal mottle virus (PVMV), a panel of monoclonal antibodies (mAbs), raised to a Nigerian isolate (PVMW‐Ni), was evaluated by double‐antibody sandwich enzyme‐linked immunosorbent assay (DAS‐ELISA) on infected pepper samples from seven West African countries.
C. Huguenot +3 more
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2021
Bu çalışmada, 2016 yılında Isparta ve Antalya illerinde fasulye tohumlarında Fasulye adi mozayik virüsü (Bean common mosaic virus: BCMV)’nün belirlenmesi amacıyla yapılmıştır. Bu amaçla araştırma alanından 106 tohum örneği toplanmıştır. Tohum örnekleri biyolojik, serolojik ve moleküler çalışmalar için kullanılmıştır.
ÇULAL KILIÇ, Handan, ÇAT, Ahmet
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Bu çalışmada, 2016 yılında Isparta ve Antalya illerinde fasulye tohumlarında Fasulye adi mozayik virüsü (Bean common mosaic virus: BCMV)’nün belirlenmesi amacıyla yapılmıştır. Bu amaçla araştırma alanından 106 tohum örneği toplanmıştır. Tohum örnekleri biyolojik, serolojik ve moleküler çalışmalar için kullanılmıştır.
ÇULAL KILIÇ, Handan, ÇAT, Ahmet
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2017
The present study was conducted to compare the sensibility of differentmethods (DAS-ELISA and RT-PCR) which are used to detect the agents of viraldiseases in grapevine. Purposive sampling was done in vineyards in Manisa andİzmir provinces and a total of 50 samples were taken from grapevines showingvirus symptoms.
ERİLMEZ, Serpil, KAYA, Aydan
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The present study was conducted to compare the sensibility of differentmethods (DAS-ELISA and RT-PCR) which are used to detect the agents of viraldiseases in grapevine. Purposive sampling was done in vineyards in Manisa andİzmir provinces and a total of 50 samples were taken from grapevines showingvirus symptoms.
ERİLMEZ, Serpil, KAYA, Aydan
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Detekce virů pícnin pomocí DAS-ELISA
2014The subject of this bachelor thesis is screening of viruses on wild and cultivated forage crop growing in the Czech Republic. Two viruses Red clover vein mosaic virus (RCVMV; genus Carlavirus) and Clover yellow mosaic virus (ClYMV; genus Potexvirus) were screened using the DAS-ELISA. Three plants of Trifolium pratense (variety Amos, Blizard and Rezista)
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Plant Disease, 1995
Skaf, J. S., and Carroll, T. W. 1995. Purification of barley yellow streak mosaic virus and detection by DAS-ELISA and ISEM using polyclonal antibodies. Plant Dis. 79:1003-1007. A purification procedure was developed for barley yellow streak mosaic virus (BaYSMV), the causal agent of a disease in barley and wheat.
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Skaf, J. S., and Carroll, T. W. 1995. Purification of barley yellow streak mosaic virus and detection by DAS-ELISA and ISEM using polyclonal antibodies. Plant Dis. 79:1003-1007. A purification procedure was developed for barley yellow streak mosaic virus (BaYSMV), the causal agent of a disease in barley and wheat.
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Food Chemistry
Peanuts are highly nutritious but pose a significant risk of triggering food allergies. While heat treatment can reduce the allergenicity of many foods, it may also alter their structure, potentially impacting detection results. This study employed double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and lateral flow ...
Shuo, Yang +7 more
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Peanuts are highly nutritious but pose a significant risk of triggering food allergies. While heat treatment can reduce the allergenicity of many foods, it may also alter their structure, potentially impacting detection results. This study employed double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and lateral flow ...
Shuo, Yang +7 more
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Detection of Virus Diseases by DAS-ELISA Method in Sugar Beet Growing Areas in Eskişehir Province
2012Bu çalışma Eskişehir ili şeker pancarı yetiştiriciliği yapılan alanlarda sorun olan virüs hastalıklarının belirlenmesi amacıyla gerçekleştirilmiştir. 2010 yılı survey çalışmalarında virüs simptomu gösteren (sararma, solgunluk, mozaik, nekroz) bitkilerden 94 yaprak örneği alınmıştır.
YARDIMCI, Nejla +2 more
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