Results 171 to 180 of about 12,751,845 (288)

A perspective on the use of personal data stores in data spaces to support multiparty data access as required by the data Act. [PDF]

Data Brief
Coenen T, Fierens M, Esteves B, De Loof E.   +3 more
europepmc   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

A principled approach to cross-sector genomic data access. [PDF]

Bioethics, 2021
Smith M, Miller S.
europepmc   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Unlocking Real-Time Data Access in Diabetes Management: Toward an Interoperability Model. [PDF]

J Diabetes Sci Technol
Randine P, Kopperstad Wolff M, Pocs M, Connell IRO, Cafazzo JA, Årsand E.   +5 more
europepmc   +1 more source

Expanding citizen engagement in the secondary use of health data: an opportunity for national health data access bodies to realise the intentions of the European health data space. [PDF]

Arch Public Health, 2023
Saelaert M, Mathieu L, Van Hoof W, Devleesschauwer B.   +3 more
europepmc   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

MetaboSERV-a platform for selecting, exchanging, and visualizing metabolomics data with controlled data access. [PDF]

Gigascience
Tucholski T, Maennel A, Njipouombe Nsangou YA, Schuchardt S, Gruber M, Kellermeier F, Dettmer K, Oefner PJ, Gronwald W, Altenbuchinger M, Dönitz J, Zacharias HU.   +11 more
europepmc   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Data access arrangements in genomic research consortia. [PDF]

Sci Rep
Nicol D, Nielsen J, Archer M.
europepmc   +1 more source