Results 41 to 50 of about 7,228 (241)

Isolation and culture of primary osteocytes from the long bones of skeletally mature and aged mice

BioTechniques, 2012
The purpose of this work was to establish a methodology to enable the isolation and study of osteocytes from skeletally mature young (4-month-old) and old (22-month-old) mice.
Amber Rath Stern, Matthew M. Stern, Mark E. Van Dyke, Katharina Jähn, Matthew Prideaux, Lynda F. Bonewald   +5 more
doaj   +1 more source

Enhancing Histological Techniques for Small Crustaceans: Evaluation of Fixation, Decalcification, and Enzymatic Digestion in <i>Neocaridina</i> Shrimp. [PDF]

Animals (Basel)
Histological techniques are essential for studying small crustaceans’, such as Neocaridina shrimp, anatomy and physiology. However, their small size and rapid tissue autolysis present challenges for fixation and processing. This study aimed to optimize histological methods for Neocaridina shrimp by evaluating different protocols for fixation ...
Wild RK, Adamek-Urbańska D, Balicki AW, Cieśla W, Przybyszewski J, Kamaszewski MG.   +5 more
europepmc   +4 more sources

Rapid decalcification technique using tissue floatation bath (TFB) in a tertiary care oncology centre

Indian Journal of Pathology and Oncology, 2016
Background: Decalcification of bone is a very important procedure in pathology especially in an oncology set up for proper staging, chemotherapy response or even in diagnosis of tumors. Objectives- To evaluate the use of tissue floatation bath (TFB) for rapid decalcification of mandibulectomy specimens in an oncology setup.
Sandeep Ojha, Amit Haritwal, Ramashankar Patel, Farah Meenai, Rajesh Chaurasia, Sonam Gupta   +5 more
openaire   +1 more source

Multi‐Technique Integration Identifies O‐GlcNAc Transferase in Fibroblast‐Like Synoviocytes as a Therapeutic Target for Rheumatoid Arthritis

Advanced Science, EarlyView.
AI‑guided single‑cell transcriptomics identifies OGT as a key regulator enriched in rheumatoid arthritis fibroblast‑like synoviocytes (RA‐FLSs). Inflammatory stimuli activate the OGT–SAP130 axis, which modulates the H3K27ac/H3K27me3 balance and epigenetically silences BTG2, promoting aggressive transformation of RA‑FLSs.
Duoli Xie, Jie Huang, Chunhao Cao, Zhenchao Tang, Xingchen Li, Jianmin Guo, Xinxin Chen, Fang Wang, Dongyi He, Jie Li, Yu Du, Bing He, Jianhua Yao, Aiping Lu, Chao Liang   +14 more
wiley   +1 more source

“UFO procedure” for massive aortic and mitral annular calcification involving left atrial and ventricular myocardium: a potential radical solution

Journal of Cardiothoracic Surgery, 2023
Background The “UFO procedure” was initially developed as a surgical option to enlarge the aortic annulus in patients requiring valve replacement. This technique can be employed to treat extensive endocarditis located in the intervalvular fibrous body ...
Leonhard Wert, Miralem Pasic, Roland Heck, Karel M. Van Praet, Jörg Kempfert, Stephan Jacobs, Volkmar Falk   +6 more
doaj   +1 more source

Murine Metatarsus Bone and Joint Collagen-I Fiber Morphologies and Networks Studied With SHG Multiphoton Imaging

Frontiers in Bioengineering and Biotechnology, 2021
Chronic inflammatory disease of bones and joints (e.g., rheumatoid arthritis, gout, etc.), but also acute bone injury and healing, or degenerative resorptive processes inducing osteoporosis, are associated with structural remodeling that ultimately have ...
Martin Vielreicher, Aline Bozec, Georg Schett, Oliver Friedrich   +3 more
doaj   +1 more source

An Injectable Metal‐Drug Supramolecular Condensate Enables Radiosensitization and Systemic Antitumor Immunity for Post‐Surgical Osteosarcoma Therapy

Advanced Science, EarlyView.
This work designed an injectable metal‐drug supramolecular condensate formed from hafnium ions and alendronate (Hf‐ALN) and loaded with the COX‐2 inhibitor celecoxib (CXB) for synergistic radioimmunotherapy of postoperative osteosarcoma. Mechanistically, upon X‐ray irradiation, Hf enhances radiation energy deposition, ROS generation, and DNA damage ...
Tianhua Xiao, Zunlei Gong, Chuke Chen, Hui Yu, Yu Cai, Can Liu, Hongkai Huang, Ying Li, Jinyuan Guan, Le Wang, Chengyun Ning, Guoxin Tan, Lei Zhou   +12 more
wiley   +1 more source

Aqueous-based tissue clearing in crustaceans

Zoological Letters, 2018
Background Investigation of the internal tissues and organs of a macroscopic organism usually requires destructive processes, such as dissection or sectioning. These processes are inevitably associated with the loss of some spatial information. Recently,
Alu Konno, Shigetoshi Okazaki
doaj   +1 more source

Transient Sleep Deprivation Induces Persistent Auditory Neuropathy via ROS‐Initiated Neuroinflammation and BK Channel Suppression

Advanced Science, EarlyView.
Sleep disturbance severity closely tracks hearing loss in a clinical cohort, yet the mechanistic link remains unclear. Acute sleep deprivation is shown to trigger transient cochlear oxidative stress that switches into a self‐sustaining neuroinflammatory state, suppressing BK channels and causing irreversible synaptopathy.
Dan Chen, Ying Ma, Lixuan Huang, Xin Chen, Fanliang Kong, Zhenzhou Qian, Mingyue Guan, Xinlin Wang, Yanyan Yang, Shasha Zhang, Peng Li, Renjie Chai   +11 more
wiley   +1 more source

Optimized Methyl methacrylate embedding of small and large undecalcified bones.

MethodsX
Methyl methacrylate (MMA) plastic embedding has been long established as a technique for the processing and histological assessment of bones. It provides the added benefit over paraffin in that it does not require decalcification of the tissue in order ...
Jackie A. Fretz, Nancy W. Troiano
doaj   +1 more source