Results 1 to 10 of about 1,814,143 (297)

Bone decalcification to assess programmed cell death ligand 1 expression in bone metastases of non-small cell lung cancers [PDF]

Journal of Bone Oncology, 2020
As for molecular alterations of lung adenocarcinoma, it is critical that pathologists are able to give PD-L1 expression status before first-line of treatment.
Guillaume Pontarollo, Cyrille B. Confavreux, Jean-Baptiste Pialat, Sylvie Isaac, Fabien Forest, Violaine Yvorel, Jean-Michel Maury, Nicolas Girard, Marie Brevet   +8 more
doaj   +6 more sources

Evaluation of techniques for human bone decalcification and amplification using sixteen STR markers

Egyptian Journal of Forensic Sciences, 2015
Efficient DNA extraction procedures, as well as accurate DNA amplification, are critical steps involved in the process of successful DNA analysis of skeletal samples.
Ajay Balayan, Abhilasha Kapoor, Garima Chaudhary, Anupuma Raina   +3 more
doaj   +5 more sources

Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis [PDF]

Scientific Reports
Bone tissue, with its complex structure, often necessitates decalcification of the hard tissue for ex vivo morphological studies. The choice of a suitable decalcification method plays a crucial role in preserving desired features and ensuring ...
Shibarjun Mandal, Ramya Motganhalli Ravikumar, Astrid Tannert, Annett Urbanek, Rustam R. Guliev, Max Naumann, Sina M. Coldewey, Uta Dahmen, Lina Carvalho, Luís Bastião Silva, Ute Neugebauer   +10 more
doaj   +4 more sources

Application of ultrasound accelerates the decalcification process of bone matrix without affecting histological and immunohistochemical analysis

Journal of Orthopaedic Translation, 2019
Background/Objectives: Decalcification of bone specimens is necessary for routine paraffin embedding and sectioning. Ethylenediaminetetraacetic acid (EDTA), a chelating agent for decalcification, maintains bone tissue integrity and histological features ...
Dick H. Chow, Lizhen Zheng, Li Tian, Kam-Sing Ho, Ling Qin, Xia Guo   +5 more
doaj   +4 more sources

Identification of Optimal Decalcification Method and Tissue Preparation Protocol for RNAscope In Situ Hybridization in Rodent Incisor Tooth [PDF]

Dentistry Journal
Background: RT-qPCR is the gold standard for quantitative gene expression analysis, but it requires homogenized tissue and thus loses spatial information.
János Konkoly, Árpád Kunka, Attila Szentágotai, Erika Lisztes, Rita Marincsák, Márk Racskó, Judit Bohács, Erika Pintér, Balázs Gaszner, Balázs István Tóth, Viktória Kormos   +10 more
doaj   +3 more sources

Optimization of ovine bone decalcification for increased cellular detail: a parametric study [PDF]

Journal of Histotechnology, 2022
There are many published methods of decalcifying bone for paraffin histology; however, the current literature lacks details regarding the processing of ovine tissue. Ovine bone tissue presents challenges, as samples are often denser and larger than other
James Johnson
exaly   +3 more sources

A novel method of decalcification of bone by heat-accelerated method with tissue floatation bath using nitric acid and formic Acid: A comparative study

Journal of International Clinical Dental Research Organization, 2022
Context: Decalcification of bone is a challenging aspect since penetration of decalcifying agents through dense alveolar bone is a slow process. Striking the right balance between speed and quality of staining is essential for accurate and timely ...
Amruta Hiraman Galphade, Aarti Mahajan, Nilima Prakash, Pradip Lakshaman   +3 more
doaj   +2 more sources

A practical protocol of processing mineralized tissue for Visium spatial transcriptomics [PDF]

Mechanobiology in Medicine
Spatial transcriptomics analysis of mineralized tissues faces significant challenges due to lengthy decalcification procedures that severely compromise RNA integrity and subsequent gene detection.
Jixing Miao, Mingjie Ma, Yuhan Guo, Ling Qin, Lutian Yao   +4 more
doaj   +2 more sources

A rapid method for the preparation of an in vitro osteoporosis model of calf vertebrae: histological and biomechanical study [PDF]

Frontiers in Bioengineering and Biotechnology
BackgroundIn vitro biomechanical testing is crucial for the preclinical assessment of novel implant designs. Given the constraints of limited supply and high costs associated with human specimens, calf spines are frequently employed as surrogates for ...
Anli Shi, Anli Shi, Yijie Liu, Qiang Ma, Jiaxin Li, Jiawang Fan, Zhaohui Ge, Zhaohui Ge   +7 more
doaj   +2 more sources

Combining High-Resolution Hard X-ray Tomography and Histology for Stem Cell-Mediated Distraction Osteogenesis

Applied Sciences, 2022
Distraction osteogenesis is a clinically established technique for lengthening, molding and shaping bone by new bone formation. The experimental evaluation of this expensive and time-consuming treatment is of high impact for better understanding of ...
Griffin Rodgers, Guido R. Sigron, Christine Tanner, Simone E. Hieber, Felix Beckmann, Georg Schulz, Arnaud Scherberich, Claude Jaquiéry, Christoph Kunz, Bert Müller   +9 more
doaj   +1 more source