Results 21 to 30 of about 1,814,143 (297)

Maintaining mRNA integrity during decalcification of mineralized tissues. [PDF]

PLoS ONE, 2013
Biomineralization of the extracellular matrix occurs inappropriately in numerous pathological conditions such as cancer and vascular disease, but during normal mammalian development calcification is restricted to the formation of the skeleton and ...
Daniele Belluoccio, Lynn Rowley, Christopher B Little, John F Bateman   +3 more
doaj   +3 more sources

Analysis of microstructure in mouse femur and decalcification effect on microstructure by electron microscopy

Journal of Analytical Science and Technology, 2010
Microstructure and decalcification effect by ethylenediaminetetraacetic acid (EDTA) on microstructure were studied for the compact bone of mouse femur by optical and electron microscopy. Especially the (002) reflection plane on the selected area electron
Taehoon Jeon, Changyeon Kim, Eunkyung Kim, Seung Won Nam, Kyung Song, Woomi Yang, Youn-Joong Kim   +6 more
doaj   +1 more source

Maintaining GFP Tissue Fluorescence through Bone Decalcification and Long-Term Storage

BioTechniques, 2002
John F. Harms, Lynn R. Budgeon, Neil D. Christensen, Danny R. Welch   +3 more
doaj   +2 more sources

One protocol to rule them all: a pilot study to identify the best fixation and decalcification approach for bone marrow biopsy immunohistochemistry. [PDF]

Pathologica
Summary Objective Standardization of the pre-analytical phases of bone marrow trephine biopsy (BM) has yet to be achieved. In particular, several fixative and decalcifying reagents with specific benefits and drawbacks are described, but only a few direct
Paudice M, Gambella A, Pigozzi S, Campora M, Pitto F, Bozzano S, Mastracci L, Grillo F.   +7 more
europepmc   +2 more sources

Endocrinopathy Causing Skeletal Decalcification and Ectopic Calcification Highlighted by Imaging Tour [PDF]

Journal of Clinical and Diagnostic Research, 2021
Primary Hyperparathyroidism (PHPT) is an endocrine disorder which is caused by increased production of Parathyroid Hormone (PTH). Hypercalcemia leads to a number of symptoms and may cause patient's morbidity and even mortality if left untreated. In the
PRK Bhargav, Sunil Kumar Kota, M Sabaretnam   +2 more
doaj   +1 more source

Driving the Mineral out Faster: Simple Modifications of the Decalcification Technique [PDF]

Journal of Clinical and Diagnostic Research, 2015
Introduction: Quicker decalcification is essential for faster diagnosis of hard tissue pathology. Heat and mechanical agitation are known to hasten decalcification. Aim: To compare the rate of decalcification, cellular and staining characteristics of
Supriya Nikita Kapila, Srikant Natarajan, Karen Boaz, Jay Ashokkumar Pandya, Shanmukha Raviteja Yinti   +4 more
doaj   +1 more source

Osteoporosis and Knowledge of Bone Decalcification in Workers of The National University of Education in Lima-Peru

, 2023
Objective. to determine the relationship between osteoporosis and knowledge of bone decalcification in workers of a national university in Lima. Materials and methods.
Huacho, Russbelt Yaulilahua-, Quispe, David Choque-, Sumarriva, Nadia Chávez-, Sanchez, Zaida Pumacayo-, Joaquín, Estelita Marlene Pareja-, Bustinza, Liliana Sumarriva-   +5 more
core   +1 more source

Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.

PLoS ONE, 2021
With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to
Alireza Saraji, Anne Offermann, Janine Stegmann-Frehse, Katharina Hempel, Duan Kang, Rosemarie Krupar, Christian Watermann, Danny Jonigk, Mark Philipp Kühnel, Jutta Kirfel, Sven Perner, Verena Sailer   +11 more
doaj   +1 more source

Preliminary Study on Fish Scale Collagen Lamellar Matrix as Artificial Cornea

Membranes, 2021
To construct a novel artificial cornea biomaterial, a method to prepare collagen lamellar matrix was developed in this study using grass carp scales as raw materials.
Guoping Cheng, Liang Chen, Huanhuan Feng, Bo Jiang, Yi Ding   +4 more
doaj   +1 more source

Modified Periodic Acid‐Schiff (PAS) Is an Alternative to Safranin O for Discriminating Bone–Cartilage Interfaces

JBMR Plus, 2023
Cartilage histomorphometry is often performed on decalcified, paraffin‐embedded bone sections, which provide versatility in staining applications from basic morphology to immunohistochemistry.
Kelsey M Kjosness, Philip L Reno, Maria A Serrat   +2 more
doaj   +1 more source