Mycetoma is a lifelong granulomatous disease of subcutaneous tissues and bones. Histopathology is a substantiated indicative method based on the assumption of a definitive diagnosis of mycetoma.
Magdi Mansour Salih
doaj +3 more sources
Proposal of an Appropriate Decalcification Method of Bone Marrow Biopsy Specimens in the Era of Expanding Genetic Molecular Study [PDF]
Background: The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses.
Sung-Eun Choi +2 more
doaj +5 more sources
Arduino Automated Microwave Oven for Tissue Decalcification [PDF]
Decalcification of hard tissues such as bone and teeth is a complex process that requires using chemicals such as acids and chelating agents. Acids act faster than chelating agents, but they have a greater risk of damaging biological samples.
Paolo Savadori +6 more
doaj +5 more sources
Understanding the Protective Effect of Phytate in Bone Decalcification Related-Diseases. [PDF]
Myo-inositol hexaphosphate (phytate; IP6) is a natural compound that is abundant in cereals, legumes, and nuts, and it can bind to crystal surfaces and disturb crystal development, acting as crystallization inhibitor. The adsorption of such inhibitors to
Sanchis P +6 more
europepmc +3 more sources
Decalcification of Breast Cancer Bone Metastases with EDTA Does Not Affect ER, PR, and HER2 Results [PDF]
In metastatic breast cancer (MBC), expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) guides treatment selection.
Jager, A. (Agnes) +14 more
core +6 more sources
Background: The aim of this study was to compare conventional and microwave-assisted decalcification of sheep bone with and without teeth and to detect any difference in tissue detail preservation, staining quality, and rate of decalcification. Materials
Niloofar Hajihoseini +2 more
doaj +2 more sources
Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples.
Diagnosis of osteocartilaginous pathologies depends on morphological examination and immunohistochemical and molecular biology analyses. Decalcification is required before tissue processing, but available protocols often lead to altered proteins and ...
Le Loarer, François +15 more
core +2 more sources
A Comparison of Conventional and Microwave Decalcification and Processing of Tooth and Mandibular Bone Specimens [PDF]
Introduction: Any laboratory procedure exposes the technician and the pathologists to the hazardous effects of chemicals. Conventional procedures like decalcification and histoprocessing employed in laboratories are labour intense and time consuming ...
A. Thirumal Raj +2 more
doaj +2 more sources
Optimization of Formic Acid-Formalin-Based Decalcification Protocol for Rat Calvarial Bone Histology
Decalcification is crucial in histological processing, particularly for studying mineralized tissues like bone. The choice of decalcification method can significantly impact the quality of histological sections and the preservation of tissue morphology ...
M. Pawde, Abhijit +12 more
core +2 more sources
Evaluation of decalcification for recovery of DNA from bone
The effect of decalcification on pulverised bone samples was studied to evaluate its value when processing reasonably good quality samples. The decalcified and non-decalcified bone samples were extracted using phenolchloroform extraction method ...
William Goodwin (17233966) +2 more
core +3 more sources

