Results 241 to 250 of about 240,068 (314)

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Vaccine sieve analysis on deep sequencing data using competing risks Cox regression with failure type subject to misclassification [PDF]

James Peng, Michal Juraska, Pamela A. Shaw, Peter B. Gilbert   +3 more
openalex   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients. [PDF]

Sci Data
Banerjee G, Papri SR, Huang H, Satapathy SK, Banerjee P.   +4 more
europepmc   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Long-Read Deep Sequencing Reveals High Rates of Multilineage Transmission and Rapid Viral Population Changes in Acute HIV Infection

Mullins JI, Deng W, Giorgi EE, Magaret CA, Rolland M, Bhattacharya T, Westfall DH, Yssel AE, Bumgarner RE, Murrell B, Ndung’u T, Robb ML, Rossenkhan R, Edlefsen PT, Dong KL, Chen L, Gwashu-Nyangiri A, Zhao H, Thebus R, Sawe F, Nitayaphan S, York T, Matten D, Murrell H, Pankow AP, Juraska M, Ludwig J, Hural J, Cohen MS, Corey L, McElrath MJ, Gilbert PB, Williamson C.   +32 more
europepmc   +1 more source

High-resolution mapping of sigma factor DNA-binding sequences using artificial promoters, RNA aptamers, and deep sequencing. [PDF]

Nucleic Acids Res
Khan EA, Rückert-Reed C, Dahiya GS, Tietze L, Fages-Lartaud M, Busche T, Kalinowski J, Shingler V, Lale R.   +8 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Deep Learning For Genomic Sequences

Nowadays, thanks to advanced techniques, such as the Next Generation Sequencing (NGS), the time and costs of DNA sequencing are constantly lowering. These kinds of techniques offer high throughput, speed and scalability, so that the amount of available data on DNA sequences is greater than ever before.
openaire   +1 more source

Patatin‐domain‐containing (phospho)lipases under control: Mammalian co‐regulators and pathogenic activation mechanisms

FEBS Open Bio, EarlyView.
Patatin domain‐containing (phospho)lipases are lipid‐hydrolyzing enzymes central to metabolism, membrane remodeling, and signaling. Their activity relies on precise co‐activation mechanisms involving protein–protein interactions and conformational rearrangements.
Noopur Dubey, Lina Riegler‐Berket, Monika Oberer   +2 more
wiley   +1 more source