Results 81 to 90 of about 220,670 (147)

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

<i>ACADVL</i> Deep Sequencing in a Case Study: Beyond the Common c.848T>C Pathogenic Variant. [PDF]

Genes (Basel)
Baldo F, Zupin L, Magnolato A, Capaci V, Bonati MT.   +4 more
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Deep Sequencing Reveals Dual Evolution of SARS-CoV-2: Insights Into Defective Genomes From Wuhan-Hu-1 Variants to Omicron Subvariants. [PDF]

J Med Virol
Campos C, Ibañez-Lligoña M, Colomer-Castell S, Gregori J, Garcia-Cehic D, Andrés C, Piñana M, González-Sánchez A, Rando-Segura A, Esperalba J, Saubí N, Cortese MF, Tabernero D, Rodriguez-Frias F, Ferrer R, Esteban JI, Kakze-van der Honing RW, Kampfraath AA, Vergara-Alert J, Segalés J, van der Poel WHM, Pumarola T, Antón A, Quer J.   +23 more
europepmc   +1 more source

Exam‐level analysis of lecture capture viewing and student exam performance

FEBS Open Bio, EarlyView.
This study asks how lecture capture relates to exam performance in biology courses with three noncumulative exams. Mixed‐effects modeling separated between‐students from within‐student effects on exam %. Students who watched more recordings earned higher grades, but viewing spikes above a student's own mean, consistent with cramming, predicted small ...
Kirk Hillsley
wiley   +1 more source

Deep sequencing of serially passaged Sudan virus in guinea pigs uncovers adaptive mutations. [PDF]

Heliyon
Emeterio K, Audet J, Zhu W, Leung A, Schulz H, He S, Drebot M, Banadyga L.   +7 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

“Big Events” and HIV Transmission Dynamics: Estimating Time Since HIV Infection from Deep Sequencing Data Among Sex Workers and Their Clients in Dnipro, Ukraine [PDF]

Viruses
Cholette F, Herpai N, McClarty L, Balakireva O, Pavlova D, Lopatenko A, Capiña R, Sandstrom P, Pickles M, Forget E, Mishra S, Becker M.   +11 more
europepmc   +2 more sources