Results 51 to 60 of about 23,224 (220)
Expression, purification and characterisation of recombinant peptide:N-glycosidase F. : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in Biochemistry at Massey University [PDF]
, 2000 PNGase F (Peptide-N4-(N-acetyl-D-glucosaminyl) asparagine amidase F) is an amidohydrolase isolated from the extracellular medium of the Gram-negative bacterium Flavobacterium meningosepticum.
Loo, Trevor Stephen
core
Crystallization and preliminary X-ray diffraction analysis of eukaryotic α2-macroglobulin family members modified by methylamine, proteases and glycosidases [PDF]
, 2015 © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Summary: α2-Macroglobulin (α2M) has many functions in vertebrate physiology. To understand the basis of such functions, high-resolution structural models of its conformations and complexes
Garcia-Ferrer, Irene +4 more
core +2 more sources
Biotechnology and Bioengineering, EarlyView.ABSTRACT Polysorbate‐degrading host cell proteins (HCPs) represent a critical challenge in the manufacturing of monoclonal antibody therapeutics due to their potential to persist during downstream processing. While their enzymatic activity has been characterized, the role of direct HCP‐mAb interactions, particularly those involving polysorbate ...
Melanie Maier +3 more
wiley +1 more source
Variations in Isoflavone During Soybean Maturation and Their Thermal Process-Dependent Conversion
AgronomyImmature soybean seeds, after steaming or boiling, are widely consumed in Northeast Asia. However, changes in isoflavones and antioxidant activities during processing at different seed stages remain underexplored.
Ji Hye Kim, Jae-Hee Kim, Seok Hyun Eom
doaj +1 more source
Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax [PDF]
, 2015 Sialidases and trans-sialidases play important roles in the life cycles of various microorganisms. These enzymes can serve nutritional purposes, act as virulence factors or mediate cellular interactions (cell evasion and invasion).
Ameloot, Paul +5 more
core +1 more source
Biotechnology and Bioengineering, EarlyView.Here, we uncovered an unexpected O‐deglycosylation activity of the C glycosyltransferase (FcCGT), forming phloretin (1) from phloretin 2'‐O‐glucoside (2). In the presence of UDP, FcCGT catalyzes the O→C rearrangement to yield nothofagin (3). Upon additional UDP‐glucose, further C‐glycosylation occurs, forming phloretin 3',5'‐di‐C‐glucoside (4 ...
Tuo Li +4 more
wiley +1 more source
International Journal of General Medicine, 2022 Huiyu Wang,1,* Dingyi Gu,1,* Datian Chen,2,* Jie Mei,1 Xuejing Yang,1 Junli Ding,1 Junying Xu,1 Meilin Wang,3 Chaoying Liu,1 Dong Hua1 1Comprehensive Cancer Center, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi ...
Wang H +9 more
doaj
Cancer Science, EarlyView.The CD90‐YY1‐SLC1A5 axis plays a significant role in gastric cancer. CD90 promotes the binding of YY1 to the SLC1A5 promoter. CD90 regulates SLC1A5‐mediated glutamine metabolism and ferroptosis. ABSTRACT CD90 (THY1) is a cell surface glycoprotein that plays a crucial role in the occurrence and development of various malignant tumors.
Zihua Zhou +5 more
wiley +1 more source
Journal of Anatomy, EarlyView.A well‐preserved 20 post‐conception week human brain was obtained and finely dissected into 18 anatomically distinct regions, including the pia mater. Each region underwent in‐depth proteomic analysis, encompassing both total protein content and post‐translational modifications.
S. Bandiera +9 more
wiley +1 more source
In vitro bioavailability and cellular bioactivity studies of flavonoids and flavonoid-rich plant extracts : questions, considerations and future perspectives [PDF]
, 2017 In vitro techniques are essential in elucidating biochemical mechanisms and for screening a wide range of possible bioactive candidates. The number of papers published reporting in vitro bioavailability and bioactivity of flavonoids and flavonoid-rich ...
Gonzales, Gerard
core +2 more sources