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Glycoside-metabolizing oxidoreductase D3dgpA from human gut bacterium

Frontiers in Bioengineering and Biotechnology
The Gfo/Idh/MocA family enzyme DgpA was known to catalyze the regiospecific oxidation of puerarin to 3”-oxo-puerarin in the presence of 3-oxo-glucose. Here, we discovered that D3dgpA, dgpA cloned from the human gut bacterium Dorea sp. MRG-IFC3, catalyzed
Heji Kim +6 more
doaj +1 more source

Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property

Molecules, 2017
Minor ginsenosides, such as compound K, Rg3(S), which can be produced by deglycosylation of ginsenosides Rb1, showed strong anti-cancer effects. However, the anticancer effects of gypenoside LXXV, which is one of the deglycosylated shapes of ginsenoside ...
Chang-Hao Cui +4 more
doaj +1 more source

Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen of Echinococcus multilocularis

Frontiers in Parasitology
Alveolar echinococcosis (AE) caused by Echinococcus multilocularis, is a severe zoonotic disease in humans. One of the major metacestode antigens of E. multilocularis is the Em2 or Em2(G11) native purified antigen.
Philipp A. Kronenberg +13 more
doaj +1 more source

Enzymatic deglycosylation of the dendrotoxin‐binding protein [PDF]

FEBS Letters, 1989
The neuronal membrane protein which binds the K+‐channel ligands dendrotoxin, mast cell degranulating peptide, and β‐bungarotoxin was purified from rat brain membranes. When analysed on 10% SDS gel electrophoresis, the purified protein contained two peptides: the toxin‐binding subunit of apparent M r, 90 000 and another peptide of M r, 38 000 ...
openaire +2 more sources

Table2_TiO2 Simultaneous Enrichment, On-Line Deglycosylation, and Sequential Analysis of Glyco- and Phosphopeptides.xlsx

, 2021
Reversible protein glycosylation and phosphorylation tightly modulate important cellular processes and are closely involved in pathological processes in a crosstalk dependent manner.
Cheng Chen (136164) +5 more
core +1 more source

Enzymatic deglycosylation of human TYR.

, 2016
A. Deglycosylation of TYR469 with PNGase. B. Deglycosylation of TYR469 with Endo Hf. M, molecular weight marker; C, non-treated protein as control; D, deglycosylated sample.
Bauke W. Dijkstra (216054) +3 more
core +1 more source

Table8_TiO2 Simultaneous Enrichment, On-Line Deglycosylation, and Sequential Analysis of Glyco- and Phosphopeptides.xlsx

, 2021
Reversible protein glycosylation and phosphorylation tightly modulate important cellular processes and are closely involved in pathological processes in a crosstalk dependent manner.
Cheng Chen (136164) +5 more
core +1 more source

Deglycosylation of HrCHT1b.

, 2014
(A) SDS-PAGE profile showing deglycosylation of HrCHT1b using trifluromethanesulphonic acid. Antifreeze activity analysis of the glycosylated and deglycosylated HrCHT1b (B) by observing ice crystal morphology (upper panel) and ice recrystallization ...
Renu Deswal (535317), Ravi Gupta (208247) +1 more
core +1 more source

Table1_TiO2 Simultaneous Enrichment, On-Line Deglycosylation, and Sequential Analysis of Glyco- and Phosphopeptides.xlsx

, 2021
Reversible protein glycosylation and phosphorylation tightly modulate important cellular processes and are closely involved in pathological processes in a crosstalk dependent manner.
Cheng Chen (136164) +5 more
core +1 more source

Table3_TiO2 Simultaneous Enrichment, On-Line Deglycosylation, and Sequential Analysis of Glyco- and Phosphopeptides.xlsx

, 2021
Reversible protein glycosylation and phosphorylation tightly modulate important cellular processes and are closely involved in pathological processes in a crosstalk dependent manner.
Cheng Chen (136164) +5 more
core +1 more source

erythropoietin
medicine
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western blotting