Results 71 to 80 of about 1,025,936 (342)
Molecular Oncology, EarlyView.This study integrates transcriptomic profiling of matched tumor and healthy tissues from 32 colorectal cancer patients with functional validation in patient‐derived organoids, revealing dysregulated metabolic programs driven by overexpressed xCT (SLC7A11) and SLC3A2, identifying an oncogenic cystine/glutamate transporter signature linked to ...
Marco Strecker +16 more
wiley +1 more source
Molecular Oncology, EarlyView.In over 50% of non‐metastatic breast cancer patients, circulating tumor cells (CTCs) along the whole epithelial‐mesenchymal transition spectrum are detected. Total CTC number and individual phenotypes relate to aggressive disease characteristics, including lymph node involvement and higher tumor proliferation. At the single‐cell level, mesenchymal CTCs
Justyna Topa +14 more
wiley +1 more source
The Pyruvate Dehydrogenase Complexes: Structure-based Function and Regulation*
Journal of Biological Chemistry, 2014 The pyruvate dehydrogenase complexes (PDCs) from all known living organisms comprise three principal catalytic components for their mission: E1 and E2 generate acetyl-coenzyme A, whereas the FAD/NAD+-dependent E3 performs redox recycling. Here we compare
M. Patel +3 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
OncoTarget, 2017 Sex steroid hormones such as estrogens and androgens are involved in the development and differentiation of the breast tissue. The activity and concentration of sex steroids is determined by the availability from the circulation, and on local conversion.
E. Hilborn, O. Stål, A. Jansson
semanticscholar +1 more source
Most autophagic cell death studies lack evidence of causality
FEBS Open Bio, EarlyView.Of 104 studies claiming autophagic cell death (ACD), only 13 demonstrated both causality and exclusion of apoptosis to confirm true ACD. Most studies relied on correlation‐level data or measured autophagy in isolation, revealing pervasive methodological shortcomings.
Ali Burak Özkaya, Yasmin Ghaseminejad
wiley +1 more source
Horizonte Médico, 2015 Objective: To determine the enzymatic biochemical parameters (glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST), alkaline phosphatase (ALP), gamma glutamyltransferase (γ-GT), and lactate dehydrogenase (LDH)) in children with ...
Jeél Moya S, Liz Pio D
doaj
The pivotal role of pyruvate dehydrogenase kinases in metabolic flexibility
Nutrition and Metabolism, 2014 Metabolic flexibility is the capacity of a system to adjust fuel (primarily glucose and fatty acids) oxidation based on nutrient availability. The ability to alter substrate oxidation in response to nutritional state depends on the genetically influenced
Shuai Zhang +4 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Anzu Nishiwaki +3 more
wiley +1 more source
浙江大学学报. 农业与生命科学版, 2002 The effect of seed priming with mixed-salt solution on germination and physiological characteristics of seedling in rice was investigated by determining percentage germination and germination index, activities of several enzymes under low-temperature (5 ℃
HE Chang-zheng +4 more
doaj +1 more source