A Comprehensive Quality Evaluation System for Complex Herbal Medicine Using PacBio Sequencing, PCR-Denaturing Gradient Gel Electrophoresis, and Several Chemical Approaches [PDF]
Frontiers in Plant Science, 2017 Herbal medicine is a major component of complementary and alternative medicine, contributing significantly to the health of many people and communities. Quality control of herbal medicine is crucial to ensure that it is safe and sound for use.
Xiasheng Zheng +12 more
doaj +2 more sources
Denaturing Gradient Gel Electrophoresis Approach for Microbial Shift Analysis in Thermophilic and Mesophilic Anaerobic Digestions [PDF]
GelsTo determine the evolution of microbial community and microbial shift under anaerobic processes, this study investigates the use of denaturing gradient gel electrophoresis (DGGE).
Pramod Pandey +2 more
doaj +2 more sources
Denaturing gradient gel electrophoresis of neonatal intestinal microbiota in relation to the development of asthma [PDF]
BMC Microbiology, 2011 Background The extended 'hygiene hypothesis' suggests that the initial composition of the infant gut microbiota is a key determinant in the development of atopic disease.
Desager Kristine N +3 more
doaj +2 more sources
Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis. [PDF]
Appl Environ Microbiol, 2011 ABSTRACT Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3′ ends of PCR primers, 16 primer pairs and fingerprints were generated per community.
Portillo MC +3 more
europepmc +5 more sources
Denaturing gradient gel electrophoresis (DGGE) as a powerful novel alternative for differentiation of epizootic ISA virus variants. [PDF]
PLoS ONE, 2012 Infectious Salmon Anemia is a devastating disease critically affecting world-wide salmon production. Chile has been particularly stricken by this disease which in all cases has been directly related with its causative agent, a novel orthomyxovirus which ...
Marisela Carmona +4 more
doaj +2 more sources
Denaturing gradient gel electrophoresis (DGGE). [PDF]
J Vis Exp, 2007 Peterson C.
europepmc +4 more sources
Denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology. [PDF]
J Clin Microbiol, 2006 ABSTRACT Bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. The aim of the present study was to show the potential of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant ...
Zijnge V +5 more
europepmc +7 more sources
Silver Staining of Denaturing Gradient Gel Electrophoresis Gels [PDF]
Clinical Chemistry, 2000 The continuing progress in the identification and characterization of genes that cause human genetic diseases has greatly increased the requests for a method that can rapidly identify mutations in these genes. The introduction of PCR has made it possible to rapidly obtain a relatively large amount of single-copy genomic DNA that can be used for ...
D, Radojkovic, J, Kusic
openaire +4 more sources
Detection of Listeria pathogens by gradient/constant denaturing gel electrophoresis [PDF]
Bulgarian Journal of Veterinary Medicine, 2018 Listeriosis is a serious, but preventable disease, and the virulence factor of this disease is produced only by the two pathogenic species L. monocytogenes and L. ivanovii in humans and/or animals.
A. Al-Mariri, L. Ramadan, L. Al-Halab
doaj +1 more source
Effect of vermicompost on changes in the bacterial community in maize rhizosphere [PDF]
Journal of Central European Agriculture, 2016 The aim of the study was to observe changes in the diversity of bacterial community in maize rhizosphere influenced by organic and mineral fertilization.
Eva HALENÁROVÁ +7 more
doaj +1 more source