Astrocytic gap junctions formed by connexin43 (Cx43) help maintain brain homeostasis. After mild traumatic brain injury (TBI), total cortical Cx43 increases and redistributes toward a soluble, non‐junctional pool with elevated hemichannel activity and increased phosphorylation at serine 368 (pCx43S368).
Carmen Muñoz‐Ballester +11 more
wiley +1 more source
Application of nested PCR-DGGE (denaturing gradient gel electrophoresis) for the analysis of ciliate communities in soils. [PDF]
Shimano S, Sambe M, Kasahara Y.
europepmc +1 more source
The sorting nexin Snx21 promotes flotillin‐mediated endocytosis
In this study, we describe a novel function of the previously uncharacterized Snx21 protein as a specific positive regulator of flotillin‐mediated endocytic trafficking. Snx21 is recruited to endosomal membranes via binding to PI(3)P, and it colocalizes with flotillins on the surface of late endosomes.
Tamás Maruzs +9 more
wiley +1 more source
Denaturing gradient gel electrophoresis (DGGE) as a powerful novel alternative for differentiation of epizootic ISA virus variants. [PDF]
Carmona M +4 more
europepmc +1 more source
TOP1MT rs2293925 is an enhancer‐active regulatory SNP that shapes mitochondrial R‐loop dynamics
This study shows how a common genetic variant of mitochondrial topoisomerase 1 (TOP1MT rs2293925) can influence mitochondrial gene regulation, DNA topology, and formation of noncanonical nucleic acid structures such as R‐loops. By linking this enhancer‐active variant to mitochondrial nucleic acid stress in cellular contexts relevant to amyotrophic ...
Dóra Varga +15 more
wiley +1 more source
Denaturing gradient gel electrophoresis of neonatal intestinal microbiota in relation to the development of asthma. [PDF]
Vael C +3 more
europepmc +1 more source
The C. elegans Shu complex (RFS‐1/RIP‐1/SWS‐1) is a DNA‐dependent ATPase that regulates RAD‐51 filaments during homologous recombination. The trimer preferentially binds 5′‐exposed DNA, remodels RAD‐51 filaments via ATP hydrolysis, and stabilizes filaments through ATP binding.
Sam Siu Hang Chu +4 more
wiley +1 more source
[A preliminary analysis of changes in composition of intestinal microbiota during infancy using polymerase chain reaction-denaturing gradient gel electrophoresis]. [PDF]
Li SS +6 more
europepmc +1 more source
The Mediator subunit SlMED25 and the co‐repressor SlSPX2 competitively bind the N‐terminal domain of the phosphate starvation response transcription factor SlPHR3 in tomato to form a sensitive molecular switch, which dynamically modulates phosphate starvation responses and maintains phosphate homeostasis, offering valuable targets for breeding low ...
Mingtong Zhai +10 more
wiley +1 more source
Denaturing gradient gel electrophoresis and barcoded pyrosequencing reveal unprecedented archaeal diversity in mangrove sediment and rhizosphere samples. [PDF]
Pires AC +7 more
europepmc +1 more source

