Results 111 to 120 of about 78,856 (294)

Denaturing Gradient Gel Electrophoresis (DGGE): A Rapid and Sensitive Technique to Screen Nucleotide Sequence Variation in Populations

open access: yesBioTechniques, 1999
We describe a rapid and sensitive method for the detection of nucleotide sequence variation that can be used for largescale screening of population markers.
Kristina M. Miller   +3 more
doaj   +1 more source

The development of a purification procedure for Peptide:n-Glycosidase A from Prunus amygdalus : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]

open access: yes, 1997
Peptide-N4-acetyl-β-glucosaminyl) asparagine amidases cleave the amide bond between N-linked glycans at N-acetylglucosamine and asparagine, liberating intact oligosaccharide chains from glycoproteins. Although PNGase A is commonly used by glycobiologists
Diepen, Angela Corinne van
core  

Selection for novel, acid-tolerant Desulfovibrio spp. from a closed Transbaikal mine site in a temporal pH- gradient bioreactor [PDF]

open access: yes, 2017
Almost all the known isolates of acidophilic or acid-tolerant sulphate-reducing bacteria (SRB) belong to the spore-forming genus Desulfosporosinus in the Firmicutes.
Antsiferov, Dimtry V.   +8 more
core   +1 more source

Xanthatin Targets CISD1 to Drive Ferroptosis and Mitophagy as a Dual Anticancer Strategy in Triple‐Negative Breast Cancer

open access: yesAdvanced Science, EarlyView.
Triple‐negative breast cancer has a poor prognosis. Xanthatin directly targets and degrades CISD1, triggering ferroptosis through iron dysregulation. The concurrently activated mitophagy synergistically amplifies ferroptotic cell death. Xanthatin also demonstrates potent in vivo anti‐tumor efficacy with minimal toxicity, highlighting its therapeutic ...
Qinwen Liu   +9 more
wiley   +1 more source

PCR-DGGE applied to microbial ecology that influence the production of OTA [PDF]

open access: yes, 2009
In tropical zones, OTA is mainly produced in coffee beans by three Aspergillus species: A. carbonarius, A. niger section Nigri and A. ochraceus section Circumdati.
Durand, Noël   +3 more
core  

Mechanical Strain‐Programmed SDC1+ Sheath Fibroblasts Trigger CXCR4hi Neutrophil‐Mediated Enthesitis in Ankylosing Spondylitis

open access: yesAdvanced Science, EarlyView.
In ankylosing spondylitis (AS), mechanical strain‐programmed SDC1+ sheath fibroblasts secrete CXCL5 to recruit neutrophils and promote the activation of CXCR4hi neutrophils, thereby exacerbating enthesitis by promoting neutrophil extracellular traps (NETs) formation. SOX5 controls the generation of SDC1+ sheath fibroblasts via super‐enhancers.
Jiajie Lin   +13 more
wiley   +1 more source

Metagenomic of Actinomycetes Based on 16S rRNA and nifH Genes in Soil and Roots of Four Indonesian Rice Cultivars Using PCR-DGGE

open access: yesHayati Journal of Biosciences, 2015
The research was conducted to study the metagenomic of actinomycetes based on 16S ribosomal RNA (rRNA) and bacterial nifH genes in soil and roots of four rice cultivars.
Mahyarudin, Iman Rusmana, Yulin Lestari
doaj   +1 more source

The role of bacteria in pine wilt disease: insights from microbiome analysis. [PDF]

open access: yes, 2018
Pine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause of the disease), its insect vector, and the host tree may be relevant for the disease mechanism.
Alves, Marta   +9 more
core   +1 more source

Dual Aptamers‐Based SETDB1 PROTACs as Effective Anti‐Tumor Strategies for Breast Cancer

open access: yesAdvanced Science, EarlyView.
This study establishes dual‐aptamer PROTACs targeting SETDB1 using a SETDB1‐specific aptamer conjugated to AS1411. The designed PROTACs penetrate cells, recruit MDM2 to degrade SETDB1, and inhibit cancer cell proliferation and migration. Remarkably, they also overcome tamoxifen resistance and enhance CD8+ T cell cytotoxicity, suppressing tumor growth ...
Yanxuan Guo   +6 more
wiley   +1 more source

Denaturing Gradient Gel Electrophoresis (DGGE) in microbial ecology: insights from freshwaters [PDF]

open access: yes, 2012
The Portuguese Foundation for the Science and Technology supported this work (PTDC/AAC-AMB/113746/2009) and S. Duarte (SFRH/BPD/47574/2008)
Cássio, Fernanda   +2 more
core   +2 more sources

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