Results 151 to 160 of about 33,491 (200)
Nicotiana benthamiana as a Source of Cowpea Mosaic Virus-Derived Particles That Specifically Package Designer RNAs. [PDF]
Peyret H +5 more
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Singlet oxygen-mediated photocatalytic generation of abasic sites in DNA. [PDF]
Yamano Y +6 more
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Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 1991
Denaturing gradient gel electrophoresis (DGGE) is increasingly being utilized in mutational detection, both in characterization of variations in genomic DNA and in the generation of mutational spectra after in vitro and in vivo mutagenesis. The basis for this electrophoretic separation technique is strand dissociation of DNA fragments in discrete ...
Eivind Hovig +2 more
exaly +5 more sources
Denaturing gradient gel electrophoresis (DGGE) is increasingly being utilized in mutational detection, both in characterization of variations in genomic DNA and in the generation of mutational spectra after in vitro and in vivo mutagenesis. The basis for this electrophoretic separation technique is strand dissociation of DNA fragments in discrete ...
Eivind Hovig +2 more
exaly +5 more sources
Denaturing Gradient Gel Electrophoresis (DGGE)
Methods in Molecular Biology, 2013Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in ...
Andrew Free
exaly +3 more sources
The sensitivity of denaturing gradient gel electrophoresis: a blinded analysis
Mutation Research - Mutation Research Genomics, 1998Denaturing gradient gel electrophoresis (DGGE) is considered one of the most sensitive and specific of the mutational scanning techniques, yet blinded analyses have not been reported. We report the results of a blinded study of the efficiency of DGGE to detect mutations in the Human Coagulation Factor IX. Two overlapping genomic DNA sequences from exon
Pablo VÍCTOR Gejman, P V Gejman
exaly +3 more sources
Analysis of kimchi microflora using denaturing gradient gel electrophoresis
International Journal of Food Microbiology, 2005A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was used to determine the microfloral composition during the fermentation of kimchi, a traditional Korean fermented vegetable food. The kimchi was fermented at 10 degrees C or 20 degrees C for 30 or 20 days, respectively.
Jong Seog Ahn +2 more
exaly +3 more sources
Mutational analysis using denaturing gradient gel electrophoresis and PCR
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1993Denaturing gradient gel electrophoresis (DGGE) separates (DNA) molecules based on their sequence. Using the proper conditions, all base-pair substitutions can be resolved from the wild-type sequence using DGGE. Polymerase chain reaction (PCR) permits rapid amplification of a given region of the genome.
Neal F Cariello +2 more
exaly +3 more sources
Detection of Mutations by Denaturing Gradient Gel Electrophoresis
Current Protocols in Human Genetics, 1998AbstractThis unit describes the procedure for determining the melting profile for a given PCR‐amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal /Temperature ...
A L, Børresen-Dale +2 more
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Discrimination of Cryptosporidium species by denaturing gradient gel electrophoresis
Parasitology Research, 2007Denaturing gradient gel electrophoresis (DGGE) was used for the discrimination of three species and one genotype of the protozoan parasite Cryptosporidium: the C. parvum, C. andersoni, C. muris, and C. muris Japanese field mouse genotype. A set of primers specific for the 18S rRNA gene of Cryptosporidium was used in the DGGE; consequently, the four ...
Masaaki, Satoh, Yutaka, Nakai
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