Results 171 to 180 of about 39,830 (289)
PROTEOMICS, EarlyView.ABSTRACT The protein sample preparation methods for shotgun proteomics are nowadays well‐established unlike the ones for whole protein analysis. The goal of my work has been to create a simple methodology which provides a single uncomplicated sample preparation tool for these two fields. Nowadays the bulk of proteomics work is done using detergents for
Alexandre Zougman
wiley +1 more source
VIEW, EarlyView.We created a coculture system using patient‐derived organoids (PDOs) and tumor‐infiltrating lymphocytes (TILs) that mimics important interactions between PDOs and TILs in the presence of various ICIs. Our findings reveal that CD62L+ T cells, activated by CD44+ cancer stem cells (CSCs), facilitate tumor regression in samples sensitive to ICIs, which is ...
Jie Chen +9 more
wiley +1 more source
Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR. [PDF]
BMC Pulm Med, 2014 Wu D +7 more
europepmc +1 more source
, 1993 We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by ...
G Muyzer +2 more
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Functional Ecology, EarlyView.Read the free Plain Language Summary for this article on the Journal blog. Abstract Associations of trees with arbuscular mycorrhizal (AM) and ectomycorrhizal (EcM) fungi often shape distinct microbial communities in soils. Whether this distinction can create different soil legacies and to what extent such legacies are correlated to phylogenetic ...
Minggang Wang +5 more
wiley +1 more source
Design and evaluation of PCR primers for denaturing gradient gel electrophoresis analysis of plant parasitic and fungivorous nematode communities. [PDF]
Microbes Environ, 2013 Kushida A.
europepmc +1 more source
Adaptation of the invasive pest Drosophila suzukii to a specialized nutritional niche
Insect Science, EarlyView.Unlike most Drosophila larvae that feed on spoiled food, Drosophila suzukii larvae thrive on ripening fruits and consequently face a low‐protein, high‐carbohydrate nutritional challenge. Comparisons of growth among D. suzukii, D. biarmipes, and D. melanogaster larvae across diets with varying protein‐to‐carbohydrate ratios demonstrate that D.
Yan Hou, Ying Zhen
wiley +1 more source
Application of nested PCR-DGGE (denaturing gradient gel electrophoresis) for the analysis of ciliate communities in soils. [PDF]
Microbes Environ, 2012 Shimano S, Sambe M, Kasahara Y.
europepmc +1 more source
, 2009 To explore the association of microbial community structure with the development of eutrophication in a large shallow freshwater lake, Lake Taihu. The bacterial and archaeal assemblages in sediments of different lake areas were analysed using denaturing ...
Lin, G. H. +7 more
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