Results 171 to 180 of about 33,491 (200)

Single Nucleotide Polymorphism Screening with Denaturing Gradient Gel Electrophoresis

2009
Denaturing gradient gel electrophoresis (DGGE) is a powerful technique for identifying DNA sequence-based differences. The method relies on the fact that double-stranded DNA molecules have unique denaturation rates that are based upon the specific nucleotide composition of the DNA sequence(s).
openaire   +2 more sources

Mutation Analysis by Denaturing Gradient Gel Electrophoresis (DGGE)

1996
The identification and characterization of single nucleotide variations in DNA still represents a common technical obstacle in the detection of DNA damage as well as in the genetic analysis of inherited disorders. The introduction of the polymerase chain reaction (PCR) (Saiki et al., 1988) has greatly facilitated this technical problem:the in vitro ...
Riccardo Fodde, Monique Losekoot
openaire   +1 more source

Comprehensive mutation detection with denaturing gradient gel electrophoresis

1998
Abstract Since the basic laboratory procedures for denaturing gradient electrophoresis and the introduction of G,C clamps have not changed significantly from previous descriptions, and they depart only slightly from conventional gel electrophoresis, we provide guidance and references to published complete instructions.
L S Lerman, Cherif Beldjord
openaire   +1 more source

Denaturing Gradient Gel Electrophoresis to Detect Methylation Changes in DNA

2004
Denaturing gradient gel electrophoresis (DGGE) is a technique that fractionates DNA molecules on the basis of their melting behavior and thereby permits the separation of DNA fragments with local variations in base composition. The separation of DNA fragments by DGGE is determined by the nucleotide sequence, rather than size. This approach is effective
openaire   +2 more sources

Denaturing gradient gel electrophoresis in marine microbial ecology

2001
Publisher Summary Genetic fingerprinting techniques are excellently suited to comparison of large numbers of samples. Genetic fingerprinting of microbial communities provides banding patterns or profiles that reflect the genetic diversity of the community.
Hendrik Schäfer, Gerard Muyzer
openaire   +1 more source

Denaturing gradient gel electrophoresis

2005
Svetlana A. Kocherginskaya, Isaac K.O. Cann, Roderick I. Mackie   +2 more
openaire   +1 more source

Denaturing Gradient Gel Electrophoresis (DGGE)

2005
Jeroen H. Roelfsema, Dorien J. M. Peters
openaire   +1 more source

Denaturing Gradient Gel Electrophoresis

2003
openaire   +2 more sources

Temperature and Denaturing Gradient Gel Electrophoresis

2010
Hartmut Peters, Peter N. Robinson
openaire   +1 more source

Analysis of the microflora in Tibetan kefir grains using denaturing gradient gel electrophoresis

Food Microbiology, 2009
Mingsheng Dong
exaly