Results 161 to 170 of about 33,491 (200)

Mutation detection by denaturing gradient gel electrophoresis (DGGE)

Human Mutation, 1994
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease-causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically ...
R, Fodde, M, Losekoot
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Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

Antonie van Leeuwenhoek, 1998
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so ...
Muyzer, G., Smalla, K.
openaire   +3 more sources

The gel edge electric field gradients in denaturing polyacrylamide gel electrophoresis

ELECTROPHORESIS, 1998
AbstractIt has previously been shown that zones of higher electric field form close to the loading end of the gel during denaturing polyacrylamide gel electrophoresis. Here we show that the field can reach up to three times its normal mean value a few cm in front of the loading wells when 44.5 mM Tris‐44.5 mM boric acid‐1 mM EDTA is used as the gel ...
C, Desruisseaux, G W, Slater, G, Drouin
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Denaturing Gradient Gel Electrophoresis

1999
Denaturing gradient gel electrophoresis (DGGE) (Abrams and Stanton 1992; Myers et al. 1987) is a technique suitable for detecting point mutations as well as small deletions and insertions of several base pairs in double stranded DNA. The method is based on the analysis of strand separation (denaturation) of DNA molecules.
openaire   +1 more source

Analysis of mutations using PCR and denaturing gradient gel electrophoresis

Environmental and Molecular Mutagenesis, 1991
AbstractDenaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on primary sequence. Under the appropriate conditions, all base pair (bp) substitutions, frame‐shifts, and deletions less than about 10 bp can be resolved from the wild type sequence using DGGE.
N F, Cariello, J A, Swenberg, A, De Bellis, T R, Skopek   +3 more
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Polymorphisms of human TAP2 detected by denaturing gradient gel electrophoresis

Human Immunology, 2003
The human transporter associated with antigen processing (TAP1 and TAP2) genes are located in the human leukocyte antigen (HLA) class II region of the genome and encode proteins that form a heterodimer essential for the transport of endogenous peptides into the endoplasmic reticulum for assembly with HLA class I molecules.
Alfred, Penfornis, Gang, Yan, Lijia, Shi, Denise L, Faustman   +3 more
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Human TAP1 polymorphisms detected by denaturing gradient gel electrophoresis

Tissue Antigens, 1997
Presentation of endogenous peptides by major histocompatibility complex class I (MHC) molecules is controlled, in part, by the Tap1 and Tap2 genes in the MHC class II region that encode a heterodimeric peptide transporter. Polymorphisms of human Tap1 in normal individuals have now been investigated systematically by denaturing gradient gel ...
L, Shi, G, Yan, Y, Fu, L, Ma, A, Penfornis, D, Faustman   +5 more
openaire   +2 more sources

Detection of mutations in insulin receptor gene by denaturing gradient gel electrophoresis

Diabetes, 1992
Denaturing gradient gel electrophoresis (DGGE) has been used to screen for mutations in the insulin receptor gene. Each of the 22 exons was amplified by the polymerase chain reaction (PCR). For each exon, one of the two PCR primers contained a guanine-cytosine (GC) clamp at its 5′ end.
BARBETTI F., GEJMAN P. V., TAYLOR S. I., RABEN N., CAMA A., BONORA, Enzo, PIZZO P., MOGHETTI, Paolo, MUGGEO, Michele, ROTH J.   +9 more
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Detection of p53 point mutations by double‐gradient, denaturing gradient gel electrophoresis

ELECTROPHORESIS, 1997
AbstractGenetic instability is a typical feature of tumor cells. This evidence has stimulated the development of rapid methods for detection of gene mutations. A new, improved protocol for denaturing gradient gel electrophoresis (DGGE), to screen for point mutations in genomic DNA, is reported: double gradient (DG) DGGE.
C, Gelfi, S C, Righetti, F, Zunino, G, Della Torre, M A, Pierotti, P G, Righetti   +5 more
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[Application of denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis in microbial molecular ecology].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2009
In recent years, denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. The technique is reliable, reproducible rapid and inexpensive.
Man-Li, Gong, Nan-Qi, Ren, De-Feng, Xing
openaire   +1 more source