Results 11 to 20 of about 33,616 (245)
Denaturing gradient gel electrophoresis (DGGE). [PDF]
J Vis Exp, 2007 Peterson C.
europepmc +4 more sources
Silver Staining of Denaturing Gradient Gel Electrophoresis Gels [PDF]
Clinical Chemistry, 2000 The continuing progress in the identification and characterization of genes that cause human genetic diseases has greatly increased the requests for a method that can rapidly identify mutations in these genes. The introduction of PCR has made it possible to rapidly obtain a relatively large amount of single-copy genomic DNA that can be used for ...
D, Radojkovic, J, Kusic
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Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis. [PDF]
Appl Environ Microbiol, 2011 ABSTRACT Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3′ ends of PCR primers, 16 primer pairs and fingerprints were generated per community.
Portillo MC +3 more
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Journal of Lipid Research, 2003 Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the β-position on agarose gels.
Dirk J. Blom +3 more
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles [PDF]
Journal of Clinical Microbiology, 2005 ABSTRACT Studies using molecular techniques have demonstrated that a culture-based approach can severely underestimate the bacterial diversity in most environments. One of the molecular techniques that has been applied in microbial ecology is denaturing gradient gel electrophoresis (DGGE).
Gafan, G P +5 more
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Double-Gradient DGGE for Optimized Detection of DNA Point Mutations
BioTechniques, 1997 A novel technique is reported for screening point mutations in genomic DNA: double gradient, denaturing gradient gel electrophoresis (DG-DGGE). Unlike conventional DGGE, which exploits a single gradient of denaturing chemicals (typically urea and ...
L. Cremonesi +4 more
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BioTechniques, 1998 Accumulation of T cells carrying identical T-cell receptors (TCR) is associated with a number of immunological and nonimmunological diseases. Therefore, it is of interest to be able to analyze complex T-cell populations for the presence of clonally ...
P. thor Straten +5 more
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Epsilonproteobacteria in Humans, New Zealand
Emerging Infectious Diseases, 2012 Using PCR–denaturing gradient gel electrophoresis, we examined 49 fecal samples from healthy volunteers and 128 diarrhea specimens to assess the distribution of Epsilonproteobacteria that might be routinely overlooked.
Angela J. Cornelius +5 more
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BioTechniques, 1998 Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models.
M.D. Ringel +4 more
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Supplementing in the diet of lactating Holstein cows may naturally produce coenzyme Q10-enriched milk [PDF]
Asian-Australasian Journal of Animal Sciences, 2018 Objective To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture ...
Gui-Seck Bae +6 more
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