Results 11 to 20 of about 78,856 (294)

Denaturing Gradient Gel Electrophoresis (DGGE) Analysis Indicating Increased Microbial Diversity in Landfill Area Near Conserved Wetland [PDF]

Nature Environment and Pollution Technology, 2023
The ecological balance of an ecosystem has a relation to its biodiversity. Although it has been established that biodiversity and ecological stability are related, generalization about the exact nature of this relation remains elusive and more so in ...
M. B. Chakraborty, S. R. Patgiri, A. S. Rahman, A. Dasgupta and G. Pegu
doaj   +1 more source

Diversity of bacteria on fresh eggshell surface

Zhongguo shipin weisheng zazhi, 2020
Objective To understand the composition of microbiota on the surface of fresh eggshell and its change over time. Methods The samples were collected from three cities and tested at different time points.
Yi SHI, Wenjuan LI, IWei SH, Huan LI, Siruo ZHANG, Huan ZHANG, Dongli LIU, Guozhu MA   +7 more
doaj   +1 more source

Temperature-Programmed Capillary Electrophoresis for Detection of DNA Point Mutations

BioTechniques, 1996
A method for unambiguous determination of point mutations in genomic DNA, based on electrophoresis in thin capillaries, is reported here. The method is based on the principle of temperature gradient gel electrophoresis (TGGE), a variant of denaturing ...
Cecilia Gelfi, Laura Cremonesi, Maurizio Ferrari, Pier Giorgio Righetti   +3 more
doaj   +1 more source

Analysis of genetic diversity of Trypanosoma cruzi: an application of riboprinting and gradient gel electrophoresis methods

Memorias do Instituto Oswaldo Cruz, 2000
Analysis of restriction fragment length polymorphism (RFLP) profiles derived from digestion of polymerase chain reaction (PCR) products of the ribosomal 18S from Trypanosoma cruzi yields a typical `riboprint' profile that can vary intraspecifically.
JR Stothard, IA Frame, HJ Carrasco, MA Miles   +3 more
doaj   +1 more source

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold, An, Andersen, Anderson, Anderson, Anderson, Anderson, Anderson, Appel, Artoni, Baczek, Baczek, Bell, Berggren, Bjellqvist, Bjellqvist, Bonner, Braun, Bravo, Bravo, Bravo, Bravo, Brown, Bunai, Bunger, Burre, Bury, Caglio, Caglio, Camacho-Carvajal, Cantin, Cargile, Cargile, Cargile, Castellanos-Serra, Cejka, Chen, Chevalier, Chevallet, Chiari, Chiari, Chick, Claverol, Cleveland, Cohen, Colinge, Corbett, Coughenour, Courchesne, Cox, Daban, Daban, Davis, Domon, Dukan, Duncan, Egen, Eng, Eravci, Essader, Fernandez-Patron, Figeys, Fritz, Garrels, Garrels, Gelfi, Gelfi, Giometti, Gordon, Gordon, Gorg, Gorg, Gorg, Groleau, Gronow, Han, Hardison, Hartinger, Heller, Henrich, Henzel, Hisabori, Hoffert, Hoffmann, Horowitz, Horth, Hunzinger, James, Johnson, Johnston, Jorgensen, Jovin, Jovin, Kasicka, Keller, Kobayashi, Krijgsveld, Krokhin, Kuroda, Kuster, Kyte, Laemmli, Lambert, Langen, Lasonder, Lengqvist, Liang, Link, Liu, Locke, Loo, Lopez, Lu, Lu, Lu, Luche, Lui, Luo, Luque-Garcia, Lyubimova, Macfarlane, Macfarlane, MacGillivray, Mackintosh, Malmstrom, Malone, Matsudaira, Mazzeo, Mets, Moritz, Nakamura, Navarre, Neuhoff, Nielsen, Novakova, O'Farrell, O'Farrell, Ogorzalek Loo, Orrick, Ortiz, Oses-Prieto, Pappin, Patton, Peng, Perkins, Petritis, Phelps, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Radola, Radola, Rais, Reid, Reynolds, Richert, Rigaut, Righetti, Righetti, Righetti, Righetti, Righetti, Ros, Rosenfeld, Sanchez, Santoni, Schagger, Schagger, Scherl, Schirle, Schluesener, Schmitt, Sevinsky, Sevinsky, Shang, Shapiro, Shen, Shimura, Shimura, Siemankowski, SimoAlfonso, Simpson, Steinberg, Steinberg, Switzer, Szponarski, Szponarski, Tabb, Tan, Tarroux, Tastet, Towbin, Tsugita, Tuszynski, Unlu, van Oostveen, Vonguyen, Voris, Wang, Wang, Washburn, Weber, Wen, West, White, Wilkins, Williams, Wiltfang, Winkler, Wise, Witze, Xiao, Xie, Xie, Yamaguchi, Yamaguchi, Yata, Yates, Young, Yu, Yuan, Zahedi, Zewert, Zischka, Zuo   +225 more
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Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia

Journal of Lipid Research, 2003
Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the β-position on agarose gels.
Dirk J. Blom, Pamela Byrnes, Sheena Jones, A.David Marais   +3 more
doaj   +1 more source

Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors [PDF]

, 2008
The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel ...
Altinbas, M., Freeman, S.A., Hollingsworth, J., Sierra-Alvarez, R., Smidt, H., Stams, A.J.M.   +5 more
core   +2 more sources

Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles [PDF]

Journal of Clinical Microbiology, 2005
ABSTRACT Studies using molecular techniques have demonstrated that a culture-based approach can severely underestimate the bacterial diversity in most environments. One of the molecular techniques that has been applied in microbial ecology is denaturing gradient gel electrophoresis (DGGE).
Gafan, G P, Lucas, V S, Roberts, G J, Petrie, A, Wilson, M, Spratt, D A   +5 more
openaire   +3 more sources

Double-Gradient DGGE for Optimized Detection of DNA Point Mutations

BioTechniques, 1997
A novel technique is reported for screening point mutations in genomic DNA: double gradient, denaturing gradient gel electrophoresis (DG-DGGE). Unlike conventional DGGE, which exploits a single gradient of denaturing chemicals (typically urea and ...
L. Cremonesi, S. Firpo, M. Ferrari, P.G. Righetti, C. Gelfi   +4 more
doaj   +1 more source

Detection and Characterization of αβ-T-Cell Clonality by Denaturing Gradient Gel Electrophoresis (DGGE)

BioTechniques, 1998
Accumulation of T cells carrying identical T-cell receptors (TCR) is associated with a number of immunological and nonimmunological diseases. Therefore, it is of interest to be able to analyze complex T-cell populations for the presence of clonally ...
P. thor Straten, A. Barfoed, T. Seremet, I. Saeterdal, J. Zeuthen, P. Guldberg   +5 more
doaj   +1 more source