Results 51 to 60 of about 1,234,769 (254)

Use of denaturing gradient gel electrophoresis to detect Actinobacteria associated with the human faecal microbiota

, 2013
With the exceptions of the bifidobacteria, propionibacteria and coriobacteria, the Actinobacteria associated with the human gastrointestinal tract have received little attention.
Clear, J. A., Hoyles, L., Clear, J.A., McCartney, A.L., McCartney, A. L.   +4 more
core   +1 more source

Patterns of marine bacterioplankton biodiversity in the surface waters of the Scotia Arc, Southern Ocean [PDF]

, 2012
Spatial patchiness in marine surface bacterioplankton populations was investigated in the Southern Ocean, where the Antarctic Circumpolar Current meets the islands of the Scotia Arc and is subjected to terrestrial input, upwelling of nutrients and ...
Billett, David S.M., Rogers, AD, Pearce, DA, Smale, Dan, Rogers, Alex, Smale, DA, Jamieson, RE, Billett, DS, Pearce, David, Billett, David, Pearce, David A., Jamieson, Rachel, Jamieson, Rachel E., Rogers, Alex D., Smale, Dan A.   +14 more
core   +1 more source

A Novel tRNA Half mt‐5’‐tiRNA‐Tyr Promotes Colorectal Cancer Proliferation via Inducing HARS2 Succinylation

Advanced Science, EarlyView.
A mitochondria‐derived tRNA half, mt‐5’‐tiRNA‐Tyr, is upregulated in colorectal cancer tissues and plasma and promotes tumor proliferation. It binds HARS2 and promotes CPT1A‐associated HARS2 K91 succinylation, disrupts HARS2–mt‐tRNA‐His association, impairs mitochondrial translation, enhances succinate‐linked metabolic reprogramming, and shows promise ...
Xinliang Gu, Danping Zhu, Xinwei Liu, Shangshang Hu, Xincheng Yang, Junjie Nie, Tao Xu, Yuqin Pan, Mu Xu, Huiling Sun, Shukui Wang   +10 more
wiley   +1 more source

Nucleoside‐Modified mRNA Encoding Alpha‐Galactosidase A Ameliorates Fabry Disease Phenotypes in Human IPSC‐Derived Cardiomyocytes

Advanced Science, EarlyView.
Human iPSC‐derived Fabry cardiomyocytes exhibited broad transcriptional dysregulation, apoptosis, mitochondrial dysfunction, impaired reactive oxygen species handling, altered contractility, and abnormal calcium transient decay, potentially mediated by phospholamban hyperphosphorylation.
Malte Juchem, Lea Oehlsen, Sedef Ersoy, Jia Li Ye, Natalie Weber, Elisa Mohr, Wilson Agyapong, Junqing Liu, Maximilian Fuchs, Ke Xiao, Christopher Jahn, Reto Eggenschwiler, Tobias Cantz, Julia Beimdiek, Falk F. R. Buettner, Theresia Kraft, Jan Hegermann, Ivonne J. Knorr, Lisa Ernst, Linda Feldbrügge, Resa Puffert, Kevin Schmidt, Christian Bär, Jeannine Hoepfner, Thomas Thum   +24 more
wiley   +1 more source

Changes in proteolytic bacteria in paddy soils in response to organic management

Acta Agriculturae Scandinavica. Section B, Soil and Plant Science, 2017
Proteolytic bacterial communities, which mineralize organic nitrogen, play a key role in agricultural systems. In this study, alkaline metalloprotease (apr) gene fragments from proteolytic bacteria were investigated in bulk and rhizosphere paddy soil ...
Shu Wang, Xiping Cheng
doaj   +1 more source

Analysis of bacterial community shifts in the gastrointestinal tract of pigs fed diets supplemented with β-glucan from Laminaria digitata, Laminaria hyperborea and Saccharomyces cerevisiae

Animal, 2013
This study was designed to evaluate the effects of algal and yeast β-glucans on the porcine gastrointestinal microbiota, specifically the community of Lactobacillus, Bifidobacterium and coliforms.
P. Murphy, F. Dal Bello, J. O'Doherty, E.K. Arendt, T. Sweeney, A. Coffey   +5 more
doaj   +1 more source

RSF1‐Dependent PAR Turnover Promotes 53BP1 Liquid Condensate Formation at DNA Damage Sites

Advanced Science, EarlyView.
At sites of DNA damage, RSF1 recruits PARG to accelerate PAR turnover, triggering a switch from PAR‐driven condensates to 53BP1 condensates. This condensate transition enables p53‐dependent gene transcription and coordinates the DNA damage response.
Yungyeong Heo, Yonghyeon Kim, Nangyeong Hwang, Gwanho Sung, Sujin Jo, Yeonhu Lee, Ho‐Chul Kang, Jae‐Ho Lee, Yong Won Choi, Hyeseong Cho, Sunwoo Min   +10 more
wiley   +1 more source

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

, 1998
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented, Furthermore, the potentials and limitations of these techniques ...
Muyzer, G., Smalla, K.
core   +1 more source

Switching Spike Plasticity Shapes ACE2 Engagement Across SARS‐CoV‐2 Variants

Advanced Science, EarlyView.
Conformational plasticity governs SARS‐CoV‐2 spike function and ACE2 recognition. Using high‐speed AFM and single‐molecule force spectroscopy, we analyzed the ancestral spike and nine variants, revealing progressive rigidification from Delta, variable plasticity in Omicron sublineages, and JN.1 compaction.
Sarah Stainer, Rong Zhu, Manuel Reithofer, Gerhard Stadlmayr, Katharina Stadlbauer, Panchika Prangkio, Christoph Rippel, Markus Hahn, Aniko Eva Kasztner, Stefanie Stohl, Petr Bednar, Daniel Ruzek, David Glück, Robert Tampé, Gordana Wozniak‐Knopp, Yoo Jin Oh, Miriam Klausberger, Peter Hinterdorfer   +17 more
wiley   +1 more source

Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase from human liver.

Journal of Lipid Research, 1982
The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase from human liver was purified to homogeneity. Electrophoresis of the purified human enzyme on non-denaturing gels revealed a single protein-staining band that co-migrated with reductase activity ...
R D Tanaka, P A Edwards, S F Lan, E M Knöppel, A M Fogelman   +4 more
doaj   +1 more source