ABSTRACT The protein sample preparation methods for shotgun proteomics are nowadays well‐established unlike the ones for whole protein analysis. The goal of my work has been to create a simple methodology which provides a single uncomplicated sample preparation tool for these two fields. Nowadays the bulk of proteomics work is done using detergents for
Alexandre Zougman
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Abundance and Diversity of Hydrogenotrophic Microorganisms in the Infant Gut before the Weaning Period Assessed by Denaturing Gradient Gel Electrophoresis and Quantitative PCR. [PDF]
Sagheddu V +3 more
europepmc +1 more source
A bioinspired siRNA nanocarrier is engineered by integrating an influenza A M2 proton channel with siRNA through a peptide nucleic acid linker on liposome surfaces. Active proton transport amplifies osmotic stress within endosomes, triggering membrane rupture and cytosolic release.
Seongmin Ga +16 more
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A Comprehensive Quality Evaluation System for Complex Herbal Medicine Using PacBio Sequencing, PCR-Denaturing Gradient Gel Electrophoresis, and Several Chemical Approaches. [PDF]
Zheng X +9 more
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A photoreversible 1D protein assembly has been developed by utilizing symmetry matching between StrepTactin and photoswitchable Dronpa145N. The light‐induced modulation of oligomerization allows for precise control over protein connectivity and valency.
Jangwon Bae, Yongwon Jung
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Dynamic changes of yak (Bos grunniens) gut microbiota during growth revealed by polymerase chain reaction-denaturing gradient gel electrophoresis and metagenomics. [PDF]
Nie Y +7 more
europepmc +1 more source
Isolation and characterization of lactic acid bacteria from fresh Chinese traditional rice wines using denaturing gradient gel electrophoresis. [PDF]
Jiao J, Zhang L, Yi H.
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Adaptation of the invasive pest Drosophila suzukii to a specialized nutritional niche
Unlike most Drosophila larvae that feed on spoiled food, Drosophila suzukii larvae thrive on ripening fruits and consequently face a low‐protein, high‐carbohydrate nutritional challenge. Comparisons of growth among D. suzukii, D. biarmipes, and D. melanogaster larvae across diets with varying protein‐to‐carbohydrate ratios demonstrate that D.
Yan Hou, Ying Zhen
wiley +1 more source
Comparative Analysis of Denaturing Gradient Gel Electrophoresis and Temporal Temperature Gradient Gel Electrophoresis Profiles as a Tool for the Differentiation of Candida Species. [PDF]
Mohammadi P, Hamidkhani A, Asgarani E.
europepmc +1 more source

