Results 131 to 140 of about 6,470,188 (388)
Journal of Extracellular VesiclesExtracellular vesicles (EVs) secreted by mammalian cells are highly heterogeneous in content and function. Whether this is also true for EVs secreted by plant cells is not yet known.
Benjamin L. Koch +5 more
doaj +1 more source
Generation of a controllable electron density gradient using a single plasma source
AIP Advances, 2019 In this work, an experimental design for generation of the controllable electron density gradient is achieved through a large area oxide coated cathode and two stainless meshes.
Yiming Ling +5 more
doaj +1 more source
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Phenotypic plasticity of European larch radial growth and wood density along a‐1,000 m elevational gradient [PDF]
, 2021 Margarita Escobar‐Sandoval +4 more
openalex +1 more source
Terrestrial, Atmospheric and Oceanic Sciences, 2009 In this paper, a two-dimensional numerical study of a plasma density gradient effect on Farley-Buneman waves (FB waves) is per formed via a two-fluid code in which the electron inertia is neglected while the ion inertia is retained.
Chi-Lon Fern Fu-Shong Kuo
doaj +1 more source
Separation of megakaryocytes from mouse bone marrow by density gradient centrifugation [PDF]
, 1976 A Nakeff, DP Floeh
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Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source
Gradient Flow Based Discretized Kohn-Sham Density Functional Theory [PDF]
, 2019 Xiaoying Dai, Qiao Wang, Aihui Zhou
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SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source