Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
FEBS Open Bio, EarlyView.Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Protocol for density gradient neutrophil isolation and flow cytometry-based characterization from human peripheral blood. [PDF]
STAR Protoc, 2023 Kuang Y, Parthasarathy U, Martinelli R.
europepmc +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Deblurring structural edges in variable thickness topology optimization via density-gradient-informed projection. [PDF]
Struct Multidiscipl OptimStankiewicz G, Dev C, Steinmann P.
europepmc +1 more source
Isolation of Hepatic and Adipose-Tissue-Derived Extracellular Vesicles Using Density Gradient Separation and Size Exclusion Chromatography. [PDF]
Int J Mol Sci, 2023 Martínez-Greene JA +3 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A comparison of sequential density ultracentrifugation and density gradient ultracentrifugation for the proteomic analysis of high-density lipoprotein. [PDF]
Front Cardiovasc MedBeazer JD +4 more
europepmc +1 more source
Comparative sperm recovery rate after density gradient centrifugation with two media for in vitro fertilization. [PDF]
JBRA Assist Reprod, 2023 Fernandes NS +6 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Isolation of small extracellular vesicles from small volumes of blood plasma using size exclusion chromatography and density gradient ultracentrifugation. [PDF]
ElifeKong F +4 more
europepmc +1 more source