Results 131 to 140 of about 638,495 (263)

Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue. [PDF]

STAR Protoc, 2023
Zhou X, Meng M, Wu Y, Gao R, Shan Y, Gu S, He J.   +6 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust. [PDF]

BMC Infect Dis
Wen L, Ning W, Zhang HP, Peng F, Wang MM, Huang FF, Zhang B, Huang JP, Xu MZ, Tian B.   +9 more
europepmc   +1 more source

Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures. [PDF]

MethodsX, 2023
Vijay S, Nair RR, Sharan D, Jakkala K, Ajitkumar P.   +4 more
europepmc   +1 more source

Normal‐Appearing White Matter Injury Mediates Chronic Deep Venous Hypoxia and Disease Progression in Multiple Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To explore how cerebral hypoxia and Normal‐Appearing White Matter (NAWM) integrity affect MS lesion burden and clinical course. Methods Seventy‐nine MS patients, including 13 clinically isolated syndrome (CIS) patients and 66 relapsing–remitting multiple sclerosis (RRMS) patients, and 44 healthy controls (HCs) were recruited from ...
Xinli Wang, Huiying Wang, Zhizheng Zhuo, Ai Guo, Ke Lv, Decai Tian, Chao Chai, Yunyun Duan, Shuang Xia   +8 more
wiley   +1 more source

Protocol for endolysosomal proteomics in goat atrial tissue using a refined density-gradient approach. [PDF]

STAR Protoc
Ayagama T, Berridge G, Fischer R, Capel RA, Verheule S, Schotten U, Burton RAB.   +6 more
europepmc   +1 more source

A comparison between the Felix™ electrophoretic system of sperm isolation and conventional density gradient centrifugation: a multicentre analysis. [PDF]

J Assist Reprod Genet, 2023
Shapouri F, Mahendran T, Govindarajan M, Xie P, Kocur O, Palermo GD, Bakos HW, Ahlström A, Caisander G, Xu B, Bai S, Lambourne S, Aitken RJ.   +12 more
europepmc   +1 more source

A Depolarizing Leak in Sodium Bicarbonate Cotransporter NBCe1 Causes Brain Edema

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives SLC4A4 encodes electrogenic sodium bicarbonate cotransporter NBCe1, prominently expressed in kidney and brain. Recessive loss‐of‐function variants in SLC4A4 cause proximal renal tubular acidosis, no brain edema. In the brain, NBCe1 is expressed by astrocytes, where it regulates pH and mediates astrocyte volume changes.
Quinty Bisseling, Mark D. Parker, Sven Kerst, Richard A. Pasternack, Jacob Tondreau, Marjolein Breur, Gemma M. van Rooijen‐van Leeuwen, Davide Tonduti, Ettore Salsano, Alejandra Darling, Joanna A. E. van Wijk, Susanna Törnroth‐Horsefield, Marianna Bugiani, Petra J. W. Pouwels, Quinten Waisfisz, Marjo S. van der Knaap, Rogier Min   +16 more
wiley   +1 more source

Enhancement of Y-bearing sperm enrichment in swamp buffalo using a TLR7/8 agonist (R848) combined with discontinuous Percoll density gradient centrifugation. [PDF]

Vet World
Dalimunthe NWY, Navanukraw P, Sirisathien S, Thammawung S, Phuektes P, Kunkitti P.   +5 more
europepmc   +1 more source

An optimized force-triggered density gradient sedimentation method for isolation of pelage follicle dermal papilla cells from neonatal mouse skin. [PDF]

Stem Cell Res Ther, 2023
Du L, Gan Y, Zheng B, Huang J, Hu Z, Miao Y, Miao Y.   +6 more
europepmc   +1 more source