Results 121 to 130 of about 3,338,865 (284)

Density Gradient Centrifugation Studies of Rubella Virus

Experimental Biology and Medicine, 1966
SummaryRubella virus was grown in three different mammalian kidney cell lines. The resulting virus-containing supernates were concentrated by forced dialysis and were then studied for density on sucrose gradients. All of the virus preparations produced peaked infectivity bands with 99% of the infec-tivity concentrated in a band from 1.040 to 1.120 g ...
openaire   +2 more sources

Melanin's Role in Balancing Mechanical Strength and Reproductive Output in Environmentally Stressed Fungal Spores

Advanced Functional Materials, EarlyView.
Melanin content directly controls fungal spore mechanical properties, with pigmented spores achieving rupture forces two orders of magnitude higher than hyaline spores. Environmental stress drives this quantity‐quality tradeoff; stressed species invest energy in melanin‐reinforced, mechanically superior thick‐walled, super‐hydrophobic spores, while ...
Atul Agrawal, Steven E. Naleway
wiley   +1 more source

A method for separating and quantifying organic and inorganic ice nucleating substances in atmospheric samples based on density gradient centrifugation [PDF]

Ice nucleating substances (INSs) influence the properties and frequencies of ice and mixed-phase clouds in the atmosphere, and hence, climate and the hydrological cycle. INSs can be classified as inorganic (e.g., mineral dust, volcanic ash) or organic (e.
Yu , Xi, Lanxiadi, Chen, Vakshan, Varatharajah, Joyce, Huang, Soleil E., Worthy, Allan K., Bertram, Gurcharan K., Uppal, Anne , Fuertes, Marie Line, Torrijos, Qianqian, Zhang, Runqiu, Song   +10 more
core   +2 more sources

Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue. [PDF]

STAR Protoc, 2023
Zhou X, Meng M, Wu Y, Gao R, Shan Y, Gu S, He J.   +6 more
europepmc   +1 more source

Biomimetic Core–Shell Liposome Nanoparticles Deplete Lipoproteins and Enhance the Purity of Extracellular Vesicles from Human Blood

Advanced Functional Materials, EarlyView.
Lipoprotein‐mimicking nanoparticles (SL‐NPs), comprising Fe3O4@SiO2 cores coated with liposomal bilayers, selectively capture lipoproteins through protein corona formation. The resulting SL‐NP‐lipoprotein complexes can be removed by low‐speed centrifugation, thereby enhancing the purity of small extracellular vesicles (sEVs) isolated from human serum ...
Man‐Di Wang, Andrew J. Perciaccante, David Inman, Bong Hwan Sung, Alissa M. Weaver, Suzanne M. Ponik, Ying Ge, Song Jin   +7 more
wiley   +1 more source

An Integrated Oxygen‐Supplying Device and Predictive Multiphysics Model for Extended Ambient Transport of Insulin‐Producing Cells

Advanced Functional Materials, EarlyView.
Cell therapies typically rely on cold‐chain logistics and cryopreservation, limiting access and compromising cell quality. Here, a dual‐chamber device separates an oxygen‐supplying chamber from a hyaluronic acid cargo chamber, sustaining oxygen delivery for over 70 h and enabling ambient‐temperature shipment.
Daniel A. Domingo‐Lopez, Ruth Tarpey, Benjamin Brennan, Bryan D. Boyle, Amir M. Alsharabasy, Abhik Mallick, Gabriella Bellavia, Abhay Pandit, Garry P. Duffy, Ruth E. Levey   +9 more
wiley   +1 more source

Assessment of Percoll density gradient centrifugation for sperm sexing in Indonesian local bulls

Cogent Food & Agriculture
This study provides a preliminary assessment of Percoll density gradient centrifugation (PDGC) in Indonesian local bulls by evaluating post-thaw sperm quality and the proportions of X- and Y-chromosome-bearing spermatozoa. Ninety frozen semen straws from
Habib Asshidiq Syah, Aulia Puspita Anugra Yekti, Nurul Isnaini, Putri Utami, Tri Eko Susilorini, Suyadi Suyadi, Mashudi Mashudi, Muhaimin Rifa’i, Anggita Dian Pramudhita, Marninphan Thongkham, Apinya Satsook, Phanuwit Paitoon, Korawan Sringarm, Trinil Susilawati   +13 more
doaj   +1 more source

A comparison between the Felix™ electrophoretic system of sperm isolation and conventional density gradient centrifugation: a multicentre analysis. [PDF]

J Assist Reprod Genet, 2023
Shapouri F, Mahendran T, Govindarajan M, Xie P, Kocur O, Palermo GD, Bakos HW, Ahlström A, Caisander G, Xu B, Bai S, Lambourne S, Aitken RJ.   +12 more
europepmc   +1 more source

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

Live motile sperm sorting device for enhanced sperm-fertilization competency: comparative analysis with density-gradient centrifugation and microfluidic sperm sorting. [PDF]

J Assist Reprod Genet, 2023
Hsu CT, Lee CI, Lin FS, Wang FZ, Chang HC, Wang TE, Huang CC, Tsao HM, Lee MS, Agarwal A.   +9 more
europepmc   +1 more source