Results 231 to 240 of about 239,867 (289)

Engineering Strain‐Stiffening Granular Hydrogels for 3D‐Printed Tissue‐Mimicry

open access: yesAdvanced Materials, EarlyView.
A 3D‐printable strain‐stiffening double‐network granular hydrogel (SDGH) enables independent, region‐specific tuning of toe (EToe) and heel (EHeel) moduli through control of microgel packing and network composition. This platform replicates tissue‐like nonlinear mechanics and allows fabrication of high‐fidelity, multilayered aortic valves with ...
Hyeokju Chae   +9 more
wiley   +1 more source

Time‐Resolved Simultaneous Mapping of Thickness and Nanoparticle Concentration in Nanofluid Thin Films via Imaging Ellipsometry and Deep‐UV Reflectance Imaging

open access: yesAdvanced Materials Interfaces, EarlyView.
This study establishes a dual‐channel optical metrology framework integrating phase‐shifting imaging ellipsometry and deep‐UV reflectance imaging. This label‐free approach enables simultaneous, time‐resolved mapping of film thickness and nanoparticle (NP) concentration in dynamic nanofluid thin films.
Eita Shoji   +3 more
wiley   +1 more source

Multilayered Digital Microfluidic Chip for Cell‐Based Assays

open access: yesAdvanced Materials Interfaces, EarlyView.
A multilayered digital microfluidic (mDMF) chip architecture is introduced to improve throughput and robustness in cell‐based assays. By multilayering electrode components and dielectric layers on glass, this design significantly reduces actuation voltage, maintains reliability, and enables expansion of the operational area with minimum current leakage.
Mert Ozden   +2 more
wiley   +1 more source
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Centrifugation of brain microsomes in a density gradient

Experimental Cell Research, 1960
Abstract Microsomal preparations from rat brain were centrifuged in various density gradients obtained with different concentrations of sucrose. The different zones of the gradients were analyzed chemically as to their protein and RNA content and examined with the electron microscope as to their content of particles and membrane structures.
V, HANZON, G, TOSCHI
openaire   +2 more sources

The Effects of Overloading in Density-Gradient Centrifugation

European Journal of Biochemistry, 1975
The effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. It is found that overloading of the gradient gives rise to particle movements which are not predictable ...
J, Steensgaard, N P, Moller, L, Funding
openaire   +2 more sources

Separation of nanorods by density gradient centrifugation

Journal of Chromatography A, 2011
The experimental conditions necessary for the synthesis of well-defined nanoparticles are often difficult to control. There is thus a compelling need for post-synthesis separation of nanoparticles polydispersed in size and shape. We demonstrate here both theoretically and experimentally that gold nanorods with diverse aspect ratios can be separated ...
Bin Xiong
exaly   +3 more sources

Zonal centrifugation in reorienting density gradients

Biochimica et Biophysica Acta (BBA) - General Subjects, 1971
Abstract 1. 1. Zonal rotors which do not require rotating-seal assemblies were tested and employed for quantitative cell fractionations in sucrose density gradients. The design and operation of these rotors represents a departure from other zonal rotors presently in use.
P, Sheeler, D M, Gross, J R, Wells
openaire   +2 more sources

Enucleation of cells by density gradient centrifugation

Experimental Cell Research, 1975
Abstract HEp-2 cells can be enucleated by ultracentrifugation in a colloidal silica (PTL) density gradient, containing cytochalasin B. Under optimal conditions, more than 70% of the cells are enucleated. Purification up to 97% is carried out by centrifugation at low speed through a second, preformed PTL density gradient.
W, Bossart, H, Loeffler, K, Bienz
openaire   +2 more sources

Density-Gradient Centrifugation

1998
In some cases, further separation of virus from the plant contaminants can be achieved by placing the clarified extract on top of a small volume of a sucrose solution in a centrifuge tube, the so-called sucrose cushion. Although the higher viscosity of the sucrose solution delays the virions reaching the bottom, the plant contaminants may be slowed ...
Jeanne Dijkstra, Cees P. de Jager
openaire   +1 more source

Computer Simulation of Density-Gradient Centrifugation

1979
Modern biochemical techniques are continuously refined to produce new and improved results. Improvements in the useful power of a biochemical technique can in principle be achieved in two ways. One is the classic trial-and-error approach whereby the parameters of the experimental conditions are varied one by one until a suitable set of conditions is ...
J, Steensgaard, N P, Møller
openaire   +2 more sources

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