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Enucleation of cells by density gradient centrifugation
Experimental Cell Research, 1975Abstract HEp-2 cells can be enucleated by ultracentrifugation in a colloidal silica (PTL) density gradient, containing cytochalasin B. Under optimal conditions, more than 70% of the cells are enucleated. Purification up to 97% is carried out by centrifugation at low speed through a second, preformed PTL density gradient.
W, Bossart, H, Loeffler, K, Bienz
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Density-Gradient Centrifugation
1998In some cases, further separation of virus from the plant contaminants can be achieved by placing the clarified extract on top of a small volume of a sucrose solution in a centrifuge tube, the so-called sucrose cushion. Although the higher viscosity of the sucrose solution delays the virions reaching the bottom, the plant contaminants may be slowed ...
Jeanne Dijkstra, Cees P. de Jager
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Computer Simulation of Density-Gradient Centrifugation
1979Modern biochemical techniques are continuously refined to produce new and improved results. Improvements in the useful power of a biochemical technique can in principle be achieved in two ways. One is the classic trial-and-error approach whereby the parameters of the experimental conditions are varied one by one until a suitable set of conditions is ...
J, Steensgaard, N P, Møller
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Density Gradient Centrifugation of a Murine Leukemia Virus
Science, 1964The Rauscher leukemia virus separated as a single band upon density gradient centrifugation in cesium chloride, rubidium chloride, sucrose, potassium citrate, or potassium tartrate. Prolonged exposure to concentrated potassium citrate or potassium tartrate solutions caused lysis of the virus; the resulting products, having different densities ...
O'conner, T E +2 more
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Centrifugation of Liver Microsomes on Metrizamide Density Gradients
Preparative Biochemistry, 1976Abstract Metrizamide, 2-(3-acetamido-5-N-methyl-acetamido-2, 4, 6–triiodobenzamido)-2-deoxy-D-glucose (mol. wt 789), inhibits liver microsomal enzymes only to a small degree and it has no solubilization or detergent effects on the membrane. Four hour centrifugation in a continuous metrizamide gradient is sufficient for microsomal vesicles to attain ...
H, Fehrnström +2 more
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An analysis of eukaryotic genomes by density gradient centrifugation
Journal of Molecular Biology, 1976DNA preparations from 25 eukaryotes, ranging from yeast to man, were analyzed in their base composition, sedimentation coefficient, modal and mean buoyant density in CsCl. Four mammalian and two amphibian DNAs were fractionated by preparative Cs 2 SO 4 −Ag + density gradient centrifugation. The CsCl band profile of each fraction was analyzed in terms
Thiery, J, Macaya, G, Bernardi, G
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Zonal separations by density-gradient centrifugation
Archives of Biochemistry and Biophysics, 1953Summary Zonal separations in the centrifuge have been obtained by floating a layer of a solution of particles on a second solution which has a density gradient and in which the particles are soluble. During subsequent centrifugation all those particles of the same size, shape, and density sedimented as a zone.
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Separation of cytoplasmic particles by centrifugation in a density-gradient
Experientia, 1953Erste Versuche zur Ultrazentrifugierung isolierter Zytoplasmapartikelfraktionen aus Krallenfroschleber (Xenopus laevis Daud.) in «Diodon»-Saccharose-Gradienten mit verschiedenen Dichtebereichen zeigen, dass mitochondrien- und mikrosomenartige Zellpartikel in verschiedenen Zonen der Gradienten ins Dichtegleichgewicht gebracht werden konnen. Die Lage der
H, HOLTER, M, OTTESEN, R, WEBER
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An Analysis of Fish Genomes by Density Gradient Centrifugation
European Journal of Biochemistry, 1980DNA was prepared from 33 species of fishes representing 12 of the 31 orders of Teleostei and one order of Chondrichthyes. DNA samples were investigated to determine modal and mean buoyant densities in CsCl (q0 and 〈q〉), main‐band asymmetry, intermolecular compositional heterogeneity and base composition.
A P, Hudson +4 more
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Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation
2023Sophisticated organelle fractionation strategies were the workhorse of early peroxisome research and led to the characterization of the principal functions of the organelle. However, even in the era of molecular biology and "omics" technologies, they are still of importance to unravel peroxisome-specific proteomes, confirm the localization of still ...
Andreas, Manner, Markus, Islinger
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